STR Training Materials
Workshop presentations by John Butler, Robin Cotton, Mecki Prinz, and Charlotte Word at the 73rd Annual American Academy of Forensic Sciences (VIRTUAL MEETING), February 16, 2021 "MVPs of Forensic DNA: Examining the Most Valuable Publications in the Field" [.pdf]
Workshop presentations by John Butler and Hari Iyer at the 31th International Symposium on Human Identification (ISHI) (VIRTUAL MEETING), September 18, 2020 "Validation Principles, Practices, Parameters, Performance Evaluations, and Protocols" [.pdf]
Workshop presentation by John Butler and Charlotte Word at the 30th International Symposium on Human Identification (ISHI) (Palm Springs, CA), September 23, 2019 "Keys to Evaluating Published Data and Summarizing Your Validation Studies" [.pdf]
Workshop presentation by John Butler, Hari Iyer and Sheila Willis at the 30th International Symposium on Human Identification (ISHI) (Palm Springs, CA), September 26, 2019 "DNA Mixture Interpretation: Principles and Best Practices" [.pdf]
John Butler workshop presented at the 28th ISFG Congress (Prague, Czech Republic), September 10, 2019, "Scientific Publication: Reading, Writing, and Reviewing" [.pdf]
Peter Vallone invited lecturer at the 28th ISFG Congress (Prague, CZ) September 9, 2019, "NGS Workflows for Forensic Genetics" [.pdf]
AAFS 2019 Workshop #10 - DNA Mixture Interpretation Principles: Observations from a NIST Scientific Foundation Review (Baltimore, MD) - February 18, 2019 [workshop page with links to individuals presentations]
Presentations for the Judicial College of Maryland course "Everything a Judge Needs to Know about the Wonderful World of DNA and Then Some" organized by Judge Sheila R. Tillerson Adams (Annapolis, MD), October 6, 2016, [Overview of DNA and What Happens in the Lab (with Bruce Heidebrecht)] [The Future of DNA] [glossary of forensic DNA terms]
AAFS 2016 Workshop #1 - Information Does Exist Beyond the First Page of Your Google Search!: Tools and Strategies for Forensic Science Literature Searching and Use (Las Vegas, NV) - February 22, 2016 [.pdf handouts] [workshop page with links to individuals presentations]
Becky Steffen, Peter Vallone, Erica Romsos, and Mike Coble presentations for "Countdown to 2017: Internal Validation of the New CODIS Loci" workshop at the International Symposium on Human Identification (Grapevine, TX), October 15, 2015 [.pdf - handouts] [Meet the New Loci Slides]
Peter Vallone presentation for the Analyzing and Utilizing Data from Next-Generation Sequencers in the Forensic Genomics Era workshop at the International Symposium on Human Identification (Grapevine, TX), October 12, 2015, "Underlying sequence variation within STRs: considerations for nomenclature, storage, searching, and reporting" [.pdf]
Mike Coble and Katherine Butler Gettings presentation for the Analyzing and Utilizing Data from Next-Generation Sequencers in the Forensic Genomics Era workshop at the International Symposium on Human Identification (Grapevine, TX), October 12, 2015, "NGS and Implications for Mixture Analysis" [.pdf]
Mike Coble and Simon Gittelson workshop for the Northeastern Association of Forensic Scientists (Allentown, PA), September 25-26, 2015, "Probabilistic DNA Mixture Interpretation Workshop" [Introduction and Overview] [Probability Theory and Likelihood Ratios] [Likelihood Ratios for Single Contributor Profiles] [Likelihood Ratios for Mixtures: Binary Approach] [Likelihood Ratios for Mixtures: Semi-Continuous Approach] [Likelihood Ratios for Mixtures: Continuous Approach] [Probabilistic Genotyping Software] [Mixture Example] [Reference Profile for Person of Interest]
John Butler and Simone Gittelson workshop at the 26th Congress of the International Society for Forensic Genetics (Krakow, Poland), August 31, 2015, "Basic STR Interpretation Workshop" [handouts]
John Butler presentation at the 26th Congress of the International Society for Forensic Genetics (Krakow, Poland), September 5, 2015, "Reviewing Scientific Literature" [.pdf]
Simone Gittelson workshop on Mixture Interpretation & Statistics at the Bode East 14th Annual DNA Technical Conference (Orlando, FL), May 29, 2015
Mike Coble, Charlotte Word, and Robin Cotton workshop at the ASCLD Symposium (Washington, DC), April 27, 2015 "An Application of the Kipling Method to DNA Validation in the 21st Century" [Workshop Overview] [Introduction to Validation] [Questions to Consider] [Validation Organization] [From Validation to SOP] [Thinking Ahead]
Mike Coble and Daniel Katz workshop at the ASCLD Symposium (Washington, DC), April 27, 2015 "DNA Mixture Interpretation" [Mixtures Past] [Maryland State Police Experience] [Mixtures Present] [Mixtures Future] [The DNA Community Experience]
John Butler organized workshop on New Autosomal and Y-STR Loci and Kits: Making Data Driven Decisions at the 25th International Symposium on Human Identification (Phoenix, AZ), October 2, 2014
NIST DNA Analyst Webinar Series: Probabilistic Genotyping and Software Programs (Part 1) (webcast May 28, 2014; 1pm to 5pm Eastern; organized by Mike Coble and John Paul Jones) taught by Susan Berdine (Denver Police Department), Todd Bille (ATF Laboratory), Mike Coble (NIST), Ate Kloosterman (Netherlands Forensic Institute), Craig O'Connor (NYC Office of Chief Medical Examiner), Charlotte Word (Consultant); for more information, see http://www.nist.gov/forensics/nist-dna-analyst-webinar-series-pt1.cfm and http://www.nist.gov/forensics/nist-dna-analyst-webinar-archive.cfm.
NIST DNA Analyst Webinar Series: Probabilistic Genotyping and Software Programs (Part 2) (webcast September 18, 2014; 11am to 2pm Eastern; organized by Mike Coble and John Paul Jones) taught by Tim Clayton (LGC Forensics, UK), Matthew Greenhalgh (Cellmark Forensics, UK), Lisa Schiermeier-Wood (Virginia Department of Forensic Sciences), Tim Kalafut (US Army Defense Forensic Science Center), and Mike Coble (NIST); for more information see http://www.nist.gov/forensics/nist-dna-analyst-webinar-series-part-2.cfm and http://www.nist.gov/forensics/dna-analyst-webinar-probabilistic-genotyping-software-programs.cfm.
John Butler presentation for NIST DNA Analyst Webinar Series: Validation Concepts and Resources - Part 1 (Gaithersburg, MD), August 6, 2014, "Validation Overview" [.pdf]
John Butler workshop at the 25th Congress of the International Society of Forensic Genetics (Melbourne, Australia), September 5, 2013, "Scientific Publication: Reading, Writing, and Reviewing" [.pdf slides] [.pdf handouts] [reference list]
ISFG 2013 Workshops on Forensic DNA Evidence Interpretation at the 25th Congress of the International Society of Forensic Genetics (Melbourne, Australia), September 2-3, 2013: Basic Principles in Forensic DNA Evidence Interpretation & Advanced Principles in Forensic DNA Evidence Interpretation
ISHI 2013 Workshop on New Autosomal and Y-STR Loci and Kits at the 24th International Symposium on Human Identification (Atlanta, GA), October 10, 2013
NIST 2013 DNA Mixture Interpretation Workshop and Webcast (April 12, 2013 8-hour webcast)
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Webcast video files: http://www.nist.gov/oles/forensics/dna-analyst-training-on-mixture-interpretation.cfm
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Slides and handouts available at NIST DNA Mixture Interpretation Webcast
Mixtures Using SOUND Statistics, Interpretation & Conclusions (ISHI 2012 Workshop: Butler, Coble, Cotton, Grgicak, Word) [Introduction] [Overview] [Profile1] [Statistics] [Profile Enhancement] [Different Assumptions] [BU Website] [Complex Mixtures] [Probabilistic Genotyping] [Complexity Threshold] [Court] [Future]
John Butler and Mike Coble workshop at the Midwestern Association of Forensic Scientists (MAFS) Fall Meeting (Milwaukee, WI), September 25, 2012, "Forensic DNA Mixture Interpretation" [Introductions] [Fundamentals& Literature] [Statistical Approaches] [Thresholds & Low-Level Mixtures] [Probabilistic Genotyping] [Literature Listing]
Mixture Interpretation: Using Scientific Analysis (ISHI 2011 Workshop: Butler, Coble, Cotton, Grgicak, Word)
Troubleshooting Laboratory Problems (ISHI 2011 Workshop: Butler and McCord)
DNA Mixture Workshop at the American Academy of Forensic Sciences meeting (Chicago, IL), February 22, 2011
Workshops at American Academy of Forensic Sciences meeting (Washington, DC), February 18-19, 2008
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Peter Vallone (chair): "qPCR: Human DNA Quantification using Real-Time PCR Assays"
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John Butler (chair): "DNA Mixture Interpretation: Principles and Practice in Component Deconvolution and Statistical Analysis"
PowerPoint slides for figures from Fundamentals of Forensic DNA Typing (3rd Edition, 1st volume) [572 slides, 39.6 Mb in total across 19 files]: [Ch. 1] [Ch. 2] [Ch. 3] [Ch. 4] [Ch. 5] [Ch. 6] [Ch. 7] [Ch. 8] [Ch. 9] [Ch. 10] [Ch. 11] [Ch. 12] [Ch. 13] [Ch. 14] [Ch. 15] [Ch. 16] [Ch. 17] [Ch. 18] [App. 3]
PowerPoint slides for figures from Advanced Topics in Forensic DNA Typing: Methodology (3rd Edition, 2nd volume) [118 slides, 4.12 Mb]
PowerPoint slides for figures from Advanced Topics in Forensic DNA Typing: Interpretation (3rd Edition, 3rd volume) [137 slides, 4.12 Mb]
PowerPoint slides for figures from Forensic DNA Typing (2nd Edition) [181 slides, 8.72 Mb file]
DNA Section Training Manual [2.5 Mb pdf file] for the Missouri State Highway Patrol Forensic Laboratory - an example of information taught, required reading, and expectations for DNA analysts and technicians in training - provided by Ruth Montgomery of the Missouri State Highway Patrol Crime Laboratory
Helpful Resources on Forensic DNA Testing (websites and glossary)
PowerPoint Presentations and Slide Shows
- Background Information (20 slides)
- STR Technology (15 slides)
- Y-Chromosomal STRs (40 slides)
from talk given by John Butler at Cambridge Healthtech Institute's Fourth Annual DNA Forensics Meeting June 1, 2000 - ISFG 2001 Talk (33 slides)
given by John Butler at 19th Congress of the International Society of Forensic Genetics (Munster, Germany) August 30, 2001 - Training on STR Typing Using Commercial Kits and ABI 310/3100
Part 1 (44 slides) Part
2 (44 slides)
Margaret C. Kline, Janette W. Redman, John M. Butler
October 22-26, 2001 - John Butler and Bruce McCord workshop at
the American Academy of Forensic Sciences (Seattle, WA), February 20, 2006
- STR Biology, Markers, and Methods (69 slides, 5.4 Mb file)
- Capillary Electrophoresis Instrumentation: Theory and Application (73 slides, 5.4 Mb)
- Validation Aspects to Consider in Bringing a New STR Kit "On-Line" (91 slides, 9.1 Mb)
- CE Troubleshooting (72 slides, 8.5 Mb)
- STR Mixture Interpretation (46 slides, 2.1 Mb)
- DNA Quantitation with Real-time qPCR and Low Copy Number Issues (63 slides, 3.8 Mb)
- Y-STRs and mtDNA (57 slides, 2.8 Mb)
PDF Versions of Presentations
(recommended for lower bandwidths; requires Adobe Acrobat; Click Adobe icon to download)
- Background Information (20 slides)
- STR Technology (15 slides)
- Y-Chromosomal STRs (40 slides)
from talk given by John Butler at Cambridge Healthtech Institute's Fourth Annual DNA Forensics Meeting June 1, 2000 - ISFG 2001 Talk (33 slides)
given by John Butler at 19th Congress of the International Society of Forensic Genetics (Munster, Germany) August 30, 2001 - Training on STR Typing Using Commercial Kits and ABI 310/3100
Part 1 (44 slides) Part
2 (44 slides)
Margaret C. Kline, Janette W. Redman, John M. Butler
October 22-26, 2001
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John Butler and Bruce McCord workshop at Northeastern Association of Forensic Scientists (Mystic, CT), September 29-30, 2004 "Capillary Electrophoresis in DNA Analysis"
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John Butler and Bruce McCord workshop for the University of Albany DNA Academy (Albany, NY), June 13-14, 2005
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Robyn Ragsdale and John Butler Validation Workshop held at the National Forensic Science Technology Center (Largo, FL), August 24-26, 2005
- [Introductions]
- [Validation Overview]
- [Introduction to DAB Standards]
- [Developmental Validation]
- [Inconsistencies in Validation Between Labs]
- [Internal Validation
- [Method Modifications and Performance Checks]
- John Butler and Bruce McCord workshop at
the American Academy of Forensic Sciences (Seattle, WA), February 20, 2006
- [STR Biology, Markers, and Methods]
- [Capillary Electrophoresis Instrumentation: Theory and Application]
- [Validation Aspects to Consider in Bringing a New STR Kit "On-Line"]
- [CE Troubleshooting]
- [STR Mixture Interpretation]
- [DNA Quantitation with Real-time qPCR and Low Copy Number Issues]
- [Y-STRs and mtDNA]
- Mike Coble - part of mtDNA Workshop held at the National Forensic Science Technology Center (Largo, FL), March 13-15, 2006
- Suni Edson
(Armed Forces DNA Identification Laboratory) -
part of mtDNA Workshop held at the
National Forensic
Science Technology Center (Largo, FL), March 13-15, 2006
- [Forensic Casework Applications of mtDNA]
- [Emerging DNA Technologies: Applications and Information to Resolve Common Mitotypes]
- Becky Hill -
part of Expert Systems Workshop at 2nd Annual Present and Future Technological
Advances in Human Identification Conference (Roanoke, VA), March 27, 2006 [NIST Experience with FSS-i3]
- John Butler and Bruce McCord workshop for
the NERFI DNA Academy II and
Massachusetts State Police Crime Laboratory (Sudbury, MA), April 27-28, 2006
- [STR Biology, Markers, and Methods]
- [Capillary Electrophoresis]
- [Validation Aspects to Consider]
- [STR Mixture Interpretation]
- [Low Copy Number DNA Testing]
- [CE Troubleshooting]
- John Butler - part of Forensic DNA Workshop at the Mid-Atlantic
Association of Forensic Scientists meeting (Richmond, VA), May 3, 2006
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John Butler - Validation Workshop at Applied Biosystem's HID University/Future Trends in Forensic DNA Technology seminar series (Albany, NY), May 10, 2006
- John Butler - workshop to New York City
Office of Chief Medical Examiner Forensic Biology section (New York City, NY),
June 6, 2006
- [General Introduction to DNA, PCR, and STRs]
- [Capillary Electrophoresis and ABI 3100]
- [Real-time qPCR DNA Quantitation and Low-Copy Number DNA Issues]
- [Mixture Interpretation]
- [Validation Studies]
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[Y-STRs and mtDNA]
- Peter Vallone and Cristian Orrego - qPCR
Workshop held at the National Forensic Science Technology Center (Largo, FL),
July 26-27, 2006
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Selected Forensic qPCR Assays: [VT Alu SYBR Green and Quantifiler] [CA DOJ nuclear/mtDNA duplex and CA DOJ degradation triplex]
- John Butler - training workshop for
Minnesota Bureau of Criminal Apprehension DNA Laboratory (St. Paul, MN),
August 7, 2006
- [Capillary Electrophoresis and ABI 3100] [CE Troubleshooting] [Expert Systems] [LCN and DNA Quantitation] [STR Mixture Interpretation] [Y-STRs and mtDNA]
- John Butler - workshop for Segunda Reunión
Internacional en Genética Forense (Tuxtla Gutiérrez, Chiapas, Mexico), August
17, 2006
- [STR DNA Analysis and Capillary Electrophoresis- English]
- [Análisis de ADN/STR y Electroforesis Capilar - Español] - translation by Lilly Moreno (Florida International University)
- John Butler and Mike Coble -
Y-Chromosome and Mitochondrial DNA Workshop
for the Northeastern Association of Forensic Scientists (NEAFS) (Rye Brook,
NY), November 1, 2006
- [Introduction] [Lineage Markers]
- Y-Chromosome [Background and Y-SNPs] [Y-STR Markers, Core Loci, Nomenclature, and Kits] [Populations, Mutations, and Statistics] [Value of Additional Loci] [Casework Examples and Resources]
- Mitochondrial DNA [Section 1] [Section 2] [Section 3] [Section 4] - not all topics covered due to lack of time
- John Butler - workshop for New Jersey State Police DNA Laboratory (Hamilton, NJ), December 5-6, 2006
- John Butler -
Houston DNA Training Workshop
(Houston, TX), April 3-4, 2007
- [CE Fundamentals] [STR Artifacts] [CE Troubleshooting]
- [Mixture Interpretation] [More Mixtures] [qPCR and Low-Copy Number DNA Testing] [miniSTRs]
- John Butler workshop for Pennsylvania
State Police DNA Lab (Greensburg, PA), June 5, 2007 "Validation Workshop
and Other Topics" [Validation]
[qPCR and LCN Issues]
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John Butler pre-conference workshops at International Society of Forensic Genetics (ISFG) meeting (Copenhagen, Denmark), August 20-21, 2007
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"Capillary Electrophoresis: Fundamentals and Troubleshooting" [.pdf]
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Validation and Implementation of New Technologies" [.pdf]
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John Butler workshop at Southern Association of Forensic Scientists (SAFS) meeting (Atlanta, GA), September 11, 2007, "Mixture Interpretation Workshop"
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John Butler workshop at Northeastern Association of Forensic Scientists (Bolton Landing, NY), November 2-3, 2007
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"The Cutting Edge of DNA Testing: Mixture Interpretation, miniSTRs, and Low Level DNA" [.pdf]
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qPCR and Mixture Interpretation Workshops at American Academy of Forensic Sciences meeting (Washington, DC), February 18-19, 2008
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Peter Vallone (chair): "qPCR: Human DNA Quantification using Real-Time PCR Assays"
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John Butler (chair): "DNA Mixture Interpretation: Principles and Practice in Component Deconvolution and Statistical Analysis"
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John Butler workshop at the CE User's Group (Ammendale, MD), April 10, 2008, "Mixture Interpretation Discussion" [.pdf]
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John Butler workshop for Florida Statewide DNA Training (Indian Rocks Beach, FL), May 12-13, 2008 "STRs, CE, and Mixtures"
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John Butler keynote presentation to the College of County Prosecutors Association of New Jersey (Jersey City, NJ), September 27, 2008. "Forensic DNA: Where Have We Come From and Where Are We Going?" [.pdf]
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John Butler and Bruce McCord workshop at the 19th International Symposium on Human Identification (Hollywood, CA), October 16, 2008, "Troubleshooting Common Laboratory Problems" [.pdf]
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John Butler and Amy Decker workshop for Armed Forces DNA Identification Laboratory (Rockville, MD), January 23, 2009, "Mixture Interpretation"
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John Butler workshop for Harris County Medical Examiner's Office DNA Laboratory (Houston, TX), January 27-28, 2009, "Mixture Interpretation and Other Topics"
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John Butler workshop for New York City Office of Chief Medical Examiner DNA Laboratory (New York City, NY), March 25, 2009, "Topics and Techniques for Forensic DNA Analysis: Continuing Education Seminar" [Introductions] [Literature Management] [Organizational Skills] [CE Instrumentation and Troubleshooting] [STR Analysis] [Mixture Interpretation]
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John Butler presentation to Towson University Forensic Statistics students (Towson, MD), April 8, 2009, "Mixture Interpretation" [.pdf]
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John Butler workshop for Wisconsin DNA Crime Lab (Milwaukee, WI), May 12, 2009, DNA Mixture Training [packet]
- John Butler workshop for Utah DNA Crime Lab (Salt Lake City, UT), May 13-14, 2009, DNA Training Seminar [packet]
- John Butler presentation at National Advocacy Center (Columbia, SC), May 20, 2009, "Addressing Questions Regarding Forensic DNA Typing" [.pdf]
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John Butler and Bruce McCord workshop at Florida International University (Miami, FL), July 20-24, 2009, "Advanced DNA Typing Workshop": [Introductions] [Intro, DNA Basics, and Historical Perspective] [DNA Extraction] [Validation and QA/QC] [DNA Quantitation] [PCR Amplification] [STR Loci and Kits] [miniSTRs] [Capillary Electrophoresis] [Data Interpretation] [Y-STRs] [Statistical Analysis] [Low Level DNA] [Mixture Interpretation] [CE Troubleshooting] [Learning with the Literature]
- John Butler and Bruce McCord workshop at
Green Mountain DNA Conference (Burlington, VT), July 27, 2009,
"Troubleshooting Common Laboratory Problems"
- John Butler pre-conference workshop on capillary electrophoresis at the International Society of Forensic Genetics (ISFG) meeting (Buenos Aires, Argentina), September 15, 2009, "Fundamentals of Capillary Electrophoresis" [.pdf]
- John Butler and Peter Vallone pre-conference workshop on validation at the International Society of Forensic Genetics (ISFG) meeting (Buenos Aires, Argentina), September 15, 2009, "Everything You Need to Know about Validation" [.pdf]
- John Butler presentation for the DNA mixture workshop at the Northwestern Association of Forensic Scientists (NWAFS) meeting (Ft. Collins, CO), September 22, 2009, "Thoughts on DNA Mixture Interpretation" [.pdf]
- John Butler workshop at Northeastern Association of Forensic Scientists (NEAFS) meeting (Long Branch, New Jersey), November 4, 2009
- DNA Mixture Workshop presentations at the American Academy of Forensic Sciences meeting (Chicago, IL), February 22, 2011, "DNA Mixture Analysis: Principles and Practice of Mixture Interpretation and Statistical Analysis Using the SWGDAM STR Interpretation Guidelines" [workshop page]
- Mike Coble presentations at the National Forensic Science Technology Center (Largo, FL), March 15-17, 2011, DNA Mixture Interpretation Workshop: [Current SWGDAM Guidelines] [Design and Execution of Validation Studies for Establishing DNA Mixture Interpretation Procedures] [Modified Procedures to Increase Sensitivity and the Impact on Mixture Interpretation Procedures]
- John Butler workshop at Indiana State Police (Indianapolis, IN), March 28, 2011, "Topics in Forensic DNA Analysis & Interpretation" [.pdf]
- Mike Coble presentations at Palm Beach County Sheriff's Office Mixture Workshop (Palm Beach, FL), April 26-27, 2011, Mixture Interpretation: [Statistics] [Case Example #3 and Software Survey]
- Mike Coble presentations for mixture workshop at Harris County Medical Examiner's Office (Houston, TX), May 9-10, 2011, "SWGDAM Guidelines and Mixture Literature", "Statistics" and "Case Example #3 and Software Survey"
- John Butler presentations for mixture workshop at Michigan State Police DNA Laboratory (Lansing, MI), May 16-17, 2011, "SWGDAM Guidelines and Mixture Literature", "Statistics" and "Case Example #3 and Software Survey"
- John Butler presentations for mixture workshop at Mesa Arizona Police Department (Mesa, AZ), June 6-7, 2011, "SWGDAM Guidelines and Mixture Literature", "Statistics" and "Case Example #3 and Software Survey"
- John Butler workshop at the International Society of Forensic Genetics (ISFG) meeting (Vienna, Austria), August 30, 2011, "Fundamentals of Capillary Electrophoresis" [.pdf]
- John Butler and Mike Coble workshop presentations for New York City Office of Chief Medical Examiner Forensic Biology (New York City, NY), April 18, 2012, "Topics and Techniques for Forensic DNA Analysis: Continuing Education Seminar"
- Mike Coble and Becky Hill workshop for The Copenhagen Forensic Genetic Summer School (Copenhagen, Denmark), June 27-28, 2012, "Advanced Topics in STR DNA Analysis"
- John Butler and Mike Coble workshop at the Midwestern Association of Forensic Scientists (MAFS) Fall Meeting (Milwaukee, WI), September 25, 2012, "Forensic DNA Mixture Interpretation" [Introductions] [Fundamentals& Literature] [Statistical Approaches] [Thresholds & Low-Level Mixtures] [Probabilistic Genotyping] [Literature Listing]
Last Updated: 10/17/2016