New STR Loci Under Development by the NIST Forensics/Human Identity Project Team

Team Members: John M. Butler (Project Leader), Margaret C. Kline, Peter M. Vallone, Michael D. Coble (at AFDIL since 17 Apr 2006), Janette W. Redman, Amy E. Decker,  Carolyn R. "Becky" Hill, David L. Duewer (Analytical Chemistry Division)

$Funding from the National Institute of Justice through the NIST Office of Law Enforcement Standards

A number of new STR loci are under development at NIST to aid forensic casework and the human identity testing community. These loci have been screened to be effective miniSTR markers (i.e., small PCR products can be generated) for use in degraded DNA analysis (see Coble and Butler JFS 2005;50(1):43-53). Eventually full locus characterization information including population data with allele frequencies will be made available on this website for each of the following markers. These autosomal STR markers were developed in triplex "NC" (non-CODIS) assays. The NC STR loci were chosen to be physically unlinked from the standard core CODIS STR loci so as to permit use of the product rule when combining data between CODIS STR and NC loci.

The NC01 loci have been recommended for adoption by European laboratories (see Gill et al. Forensic Sci. Int. 2006;156:242-244) with D2S441 replacing D14S1434 (Gill et al. Forensic Sci. Int. 2006;163:155-157). Thus, the proposed future core loci include D2S441, D10S1248, and D22S1045.


Autosomal miniSTR Loci (locus hyperlinks to STR Fact Sheet)

See Hill et al. (2006) poster for additional information on these 26 loci

Please note nomenclature changes from the original Coble and Butler (2005) publication.

NC01 (protocol): [D10S1248] [D14S1434] [D22S1045]

NC02: [D4S2364] [D2S441] [D1S1677]

Additional 20 miniSTR loci: [D3S3053] [D6S474] [D20S482] [D1GATA113] [D2S1776] [D4S2408] [D1S1627] [D5S2500] [D8S1115] [D3S4529] [D6S1017] [D9S2157] [D9S1122] [D10S1435] [D12ATA63] [D17S1301] [D18S853] [D20S1082] [D17S974] [D11S4463]


Results of the 26 NC miniSTR loci with standard samples, such as K562, 9947A, 9948, and 007.

           See Kline et al. (2006) poster for additional information

For more information, see:

Coble, M.D. and Butler, J.M. (2005) Characterization of new miniSTR loci to aid analysis of degraded DNA. J. Forensic Sci. 50: 43-53.

Mike Coble talk at the International Society of Forensic Genetics meeting (Ponta Delgada, Azores, Portugal), September 14, 2005, "Characterization and performance of new miniSTR loci for typing degraded samples" [.pdf]

Coble, M.D., Hill, C.R., Vallone, P.M., Butler, J.M. (2006) Characterization and performance of new miniSTR loci for typing degraded samples. Progress in Forensic Genetics 11, Elsevier Science: Amsterdam, The Netherlands, International Congress Series 1288, 504-506.

Gill, P., Fereday, L., Morling, N., Schneider, P.M. (2006) The evolution of DNA databases--recommendations for new European loci. Forensic Sci. Int. 156:242-244.

Gill, P., Fereday, L., Morling, N., Schneider, P.M. (2006) Letter to editor -- New multiplexes for Europe-amendments and clarification of strategic development. Forensic Sci Int. 163:155-157.

Becky Hill poster at 58th Annual Meeting of the American Academy of Forensic Sciences (Seattle, WA), February 24, 2006, "Development of 27 New miniSTR Loci for Improved Analysis of Degraded DNA Samples" [.pdf]

Mike Coble on-line presentation for the Forensic E-symposium (, February 28, 2006, "miniSTR's for low copy number and degraded DNA" [.pdf]

Mike Coble presentation at the NIJ DNA Grantees meeting (Crystal City, VA), June 26, 2006, "Development, Characterization and Performance of New miniSTR Loci for Typing Degraded Samples (on behalf of NIST)" [.pdf]

Becky Hill poster at 17th International Symposium on Human Identification (Nashville, TN), October 10-12, 2006, "Characterization of 26 New miniSTR Loci" [.pdf]

Margaret Kline poster at 17th International Symposium on Human Identification (Nashville, TN), October 10-12, 2006, "NIST SRM Updates: Value-added to the Current Materials in SRM 2391b and SRM 2395" [.pdf]

Butler, J.M. (2006) MiniSTRs: past, present, and future. Forensic News (Applied Biosystems), October 2006 [.pdf]

Dixon, L.A., Dobbins, A.E., Pulker, H., Butler, J.M., Vallone, P.M., Coble, M.D., Parson, W., Berger, B., Grubweiser, P., Mogensen, H.S., Morling, N., Nielsen, K., Sanchez, J.J., Petkovski, E., Carracedo, A., Sanchez-Diz, P., Brion, M., Irwin, J.A., Just, R.S., Loreille, O., Parsons, T.J., Syndercombe-Court, D., Schmitter, H., Gill, P. (2006) Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise. Forensic Sci. Int. 164: 33-44.

Hill, C.R., Kline, M.C., Coble, M.D., Butler, J.M. (2006) Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples. J. Forensic Sci., in press.


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