Advanced Topics in DNA Training Workshop

for the New Jersey State Police DNA Laboratory

John M. Butler (NIST)

Hamilton, NJ; December 5-6, 2006

Workshop Outline

Handouts for PowerPoint Presentations


ABI 310/3100/3130 Capillary Electrophoresis Fundamentals

CE Troubleshooting

Mixture Interpretation

qPCR and Low-Copy Number DNA Testing


Validation Essentials

Fundamentals of Statistics and Population Genetics

Mitochondrial DNA



Reference Materials    [list of key references on the ABI 310 and STR typing]

Butler, J.M., Buel, E., Crivellente, F., McCord, B.R. (2004) Forensic DNA typing by capillary electrophoresis: using the ABI Prism 310 and 3100 Genetic Analyzers for STR analysis. Electrophoresis, 25: 1397-1412.

Butler, J.M. (2006) Debunking some urban legends surrounding validation within the forensic DNA community. Profiles in DNA (Promega Corporation), vol. 9(2), pp. 3-6; see also

Butler, J.M. (2006) MiniSTRs: past, present, and future. Forensic News (Applied Biosystems), October 2006 [.pdf]

Butler, J.M. (2006) Genetics and genomics of core STR loci used in human identity testing. J. Forensic Sci. 51(2): 253-265.


Butler, J.M. (2003) Recent developments in Y-short tandem repeat and Y-single nucleotide polymorphism analysis. Forensic Sci. Rev. 15:91-111.


McCord, B. (2003) Troubleshooting capillary electrophoresis systems. Profiles in DNA 6(2): 10-12 (Promega Corporation); available at


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