Florida Statewide DNA Training Workshop


STRs, CE, and Mixtures

 

Two-day workshop for Statewide DNA Training

May 12-13, 2008 - Indian Rocks Beach, FL

 

John Butler (NIST)

[full workshop handouts-82 pages] [full workshop with articles - 135 pages]


May 12 (Monday)

 

Introductions

9:00 a.m. – 9:15 a.m.

 

STRs and Molecular Biology Artifacts [article]

9:15 a.m. – 10:30 a.m.

 

BREAK    10:30 a.m. – 10:45 a.m.    

 

miniSTRs [article]

10:45 a.m. – 11:00 a.m.

 

CE Fundamentals and Troubleshooting [article]

11:00 a.m. – 12:00 p.m.

 

LUNCH    12:00 p.m. – 1:15 p.m.

 

AFTERNOON - Dawn Herkenham on Legal Issues

 

Homework

 

May 13 (Tuesday)

 

  Background and Introductory Information

9:00 a.m. – 9:30 a.m.

 

Principles of Mixture Interpretation [article]

9:30 a.m. – 10:30 a.m.

 

BREAK    10:30 a.m. – 10:45 a.m.    

 

Strategies for Mixture Deconvolution with Worked Examples [article]

10:45 a.m. – 12:00 p.m.

 

LUNCH    12:00 p.m. – 1:15 p.m.

 

Mixture Interpretation - Statistics

1:15 p.m. – 2:00 p.m.

 

Variability between Labs in Approaches and Summaries of Mixture Interlaboratory Studies

2:00 p.m. – 3:00 p.m.

    

Presentations by Applied Biosystems and Qiagen

 

References:

 

Butler, J.M. (2006) Genetics and genomics of core STR loci used in human identity testing. J. Forensic Sci. 51(2): 253-265.

 

Hill, C.R., Kline, M.C., Mulero, J.J., Lagace, R.E., Chang, C.-W., Hennessy, L.K., Butler, J.M. (2007) Concordance study between the AmpFlSTR MiniFiler PCR Amplification Kit and conventional STR typing kits. J. Forensic Sci. 52(4): 870-873.

 

Butler, J.M., Buel, E., Crivellente, F., McCord, B.R. (2004) Forensic DNA typing by capillary electrophoresis: using the ABI Prism 310 and 3100 Genetic Analyzers for STR analysis. Electrophoresis, 25: 1397-1412.

 

Mixture Literature List