Published PCR Primers for Amplifying STR Loci

The polymerase chain reaction (PCR) relies upon the binding characteristics of the oligonucleotide primers used to define the targeted region of DNA. A great deal of effort is often expended to design primers which bind specifically to the region of interest and which do not form primer dimers (i.e., bind to themselves). Since the positions of the primers define the size of the PCR products, primers are sometimes redesigned when STR multiplexes are prepared. Different groups have often designed unique primers for the same STR loci. The STR fact sheets take this into account by listing each primer set with the subsequent PCR product sizes. Here we have broken up the published PCR primers into two groups.

British Forensic Science Service Primers

Caskey/Promega/Other Primers

Commercial Kit Primer Sequences

Promega PowerPlex 1.1 and 1.2 Kits, PowerPlex 16 Kit

Applied Biosystems considers their primer sequences proprietary and have not published them