AAFS 2008 qPCR Workshop
qPCR: Human DNA Quantification using Real-Time PCR Assays
Full-day workshop at AAFS meeting in
Monday, February 18, 2008 - Marriott Wardman Park Hotel
Chairs: Peter M. Vallone (NIST) and Eric Buel
(VT)
Co-Chairs: Margaret C. Kline
(NIST) and David R. Foran (MSU)
Planned Agenda - CURRENT MATERIALS ARE IN FINAL COLOR HANDOUT PDF FORM
Morning Session
Introduction
and Fundamentals of qPCR
[***Literature listing***]
8:30 a.m. – 9:30 a.m. – Peter M. Vallone
qPCR Sources of Variability: How Can They Be Minimized?
How
to use SRM 2372 to calibrate your qPCR standards
9:30 a.m. – 10:15 a.m. – Margaret Kline
BREAK 10:15 a.m. – 10:30 a.m.
Applying Real-Time PCR to Solve Forensic Problems
10:30 a.m. – 11:30 a.m. – Eric Buel & Jan Nicklas
Multiplex qPCR Assays at the California DOJ: Diagnosing DNA in Challenging Samples
11:30 a.m. – 12:15 p.m. – Mark D. Timken
Questions
12:15 a.m. – 12:30 p.m. – Peter M. Vallone and Panel
LUNCH 12:00 p.m. – 1:30 p.m.
Afternoon Session
The CFS-humRT qPCR Assay: Developmental Validation, Casework Experience and Lessons Learned
1:30 p.m. – 2:15 p.m. – Melanie Richard
Quantification of nuclear and mitochondrial DNA
2:15 p.m. – 3:00 p.m. – Marie Allen
BREAK 3:00 p.m. – 3:15 p.m.
qPCR at AFDIL: Our Experiences Quantitating mtDNA and more
3:15 p.m. – 4:00 p.m. – Toni M. Diegoli
Identifying Stains or Tissues as Human or Non-Human
4:00 p.m. – 4:45 p.m. – David R. Foran
Overview and Questions 4:45 p.m. – 5:00 p.m. – as needed