PowerPlex® 16 Primer Pairs
Published in Krenke, B. E., Tereba, A., Anderson, S. J., Buel, E., Culhane, S., Finis, C. J., Tomsey, C. S., Zachetti, J. M., Masibay, A., Rabbach, D. R., Amiott, E. A., and Sprecher, C. J. (2002) Validation of a 16-locus fluorescent multiplex system. J. Forensic Sci. 47(4): 773-785.
|
Locus |
Forward (top row) & Reverse (bottom row) Oligonucleotide Sequences |
5’ end |
|
FGA Primer Pair (TMR) |
GGCTGCAGGGCATAACATTA |
TMR |
|
|
ATTCTATGACTTTGCGCTTCAGGA |
OH |
|
TPOX Primer Pair (TMR) |
GCACAGAACAGGCACTTAGG |
OH |
|
|
CGCTCAAACGTGAGGTTG |
TMR |
|
D8S1179 Primer Pair (TMR) |
ATTGCAACTTATATGTATTTTTGTATTTCATG |
OH |
|
ACCAAATTGTGTTCATGAGTATAGTTTC |
TMR |
|
|
vWA Primer Pair (TMR) |
GCCCTAGTGGATGATAAGAATAATCAGTATGTG |
OH |
|
GGACAGATGATAAATACATAGGATGGATGG |
TMR |
|
|
Amelogenin Primer Pair (TMR) |
CCCTGGGCTCTGTAAAGAA |
TMR |
|
ATCAGAGCTTAAACTGGGAAGCTG |
OH |
|
|
Penta E Primer Pair (Fluorescein) |
ATTACCAACATGAAAGGGTACCAATA |
OH |
|
TGGGTTATTAATTGAGAAAACTCCTTACAATTT |
FL |
|
|
D18S51 Primer Pair (Fluorescein) |
TTCTTGAGCCCAGAAGGTTA |
FL |
|
ATTCTACCAGCAACAACACAAATAAAC |
OH |
|
|
D21S11 Primer Pair (Fluorescein) |
ATATGTGAGTCAATTCCCCAAG |
OH |
|
TGTATTAGTCAATGTTCTCCAGAGAC |
FL |
|
|
TH01 Primer Pair (Fluorescein) |
GTGATTCCCATTGGCCTGTTC |
FL |
|
ATTCCTGTGGGCTGAAAAGCTC |
OH |
|
|
D3S1358 Primer Pair (Fluorescein) |
ACTGCAGTCCAATCTGGGT |
OH |
|
ATGAAATCAACAGAGGCTTGC |
FL |
|
|
Penta D Primer Pair (JOE) |
GAAGGTCGAAGCTGAAGTG |
JOE |
|
ATTAGAATTCTTTAATCTGGACACAAG |
OH |
|
|
CSF1PO Primer Pair (JOE) |
CCGGAGGTAAAGGTGTCTTAAAGT |
JOE |
|
ATTTCCTGTGTCAGACCCTGTT |
OH |
|
|
D16S539 Primer Pair (JOE) |
GGGGGTCTAAGAGCTTGTAAAAAG |
OH |
|
GTTTGTGTGTGCATCTGTAAGCATGTATC |
JOE |
|
|
D7S820 Primer Pair (JOE) |
ATGTTGGTCAGGCTGACTATG |
JOE |
|
GATTCCACATTTATCCTCATTGAC |
OH |
|
|
D13S317 Primer Pair (JOE) |
ATTACAGAAGTCTGGGATGTGGAGGA |
OH |
GGCAGCCCAAAAAGACAGA |
JOE |
|
|
D5S818 Primer Pair (JOE) |
GGTGATTTTCCTCTTTGGTATCC |
OH |
|
AGCCACAGTTTACAACATTTGTATCT |
JOE |