STR Fact Sheet--TPOX

TPOX

Other Names	Chromosomal Location	GenBank Accession
hTPO, TPO UniSTS: 240638	2p25.3; intron 10 of human thyroid peroxidase gene Chr 2; 1.472 Mb (May 2004, NCBI build 35)	M68651; has 11 repeats

Repeat: [AATG] = GenBank top strand (commonly used)

Reported Primers	Ref.	PCR Primer Sequences
Set 1	153	5'-ACTGGCACAGAACAGGCACTTAGG-3' (AATG strand) 5'-GGAGGAACTGGGAACCACACAGGT-3' (TTAC strand)
Set 2	87	5'-CACTAGCACCCAGAACCGTC-3' (AATG strand) 5'-CCTTGTCAGCGTTTATTTGCC-3' (TTAC strand)
Set 3	Promega	PowerPlex 1.1 (TMR labeled) primer sequences 5'-ACTGGCACAGAACAGGCACTTAGG-3' 5'-[TMR]-GGAGGAACTGGGAACCACACAGGTTA-3'
Set 4	ABI	COfiler (JOE labeled), Identifiler (NED labeled)
Set 5	Promega	PowerPlex 2.1, PowerPlex 16 (TMR labeled) primer sequences 5'-GCACAGAACAGGCACTTAGG-3' 5'-[TMR]-CGCTCAAACGTGAGGTTG-3'

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1,3	Set 2	Set 4	Set 5	Repeat Structure	Ref.
4	216 bp	98 bp	209 bp	254 bp		variant allele
5	220 bp	102 bp	213 bp	258 bp		variant allele
6	224 bp	106 bp	217 bp	262 bp	[AATG]₆	185
7	228 bp	110 bp	221 bp	266 bp	[AATG]₇	284
7.3	231 bp	113 bp	224 bp	269 bp		variant allele
8	232 bp	114 bp	225 bp	270 bp	[AATG]₈	28
9	236 bp	118 bp	229 bp	274 bp	[AATG]₉	28
10	240 bp	122 bp	233 bp	278 bp	[AATG]₁₀	28
10.1	241 bp	123 bp	234 bp	279 bp		variant allele
10.3	243 bp	125 bp	236 bp	281 bp	_{1 bp deletion in flanking region}	variant allele
11	244 bp	126 bp	237 bp	282 bp	[AATG]₁₁	28
12	248 bp	130 bp	241 bp	286 bp	[AATG]₁₂	28
13	252 bp	134 bp	245 bp	290 bp	[AATG]₁₃	284
13.1	253 bp	135 bp	246 bp	291 bp		variant allele
14	256 bp	138 bp	249 bp	294 bp	[AATG]₁₄	153
15	260 bp	142 bp	253 bp	298 bp		variant allele
16	264 bp	146 bp	257 bp	302 bp		variant allele

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: PowerPlex 1.1, PowerPlex 2.1, PowerPlex 16, COfiler, Identifiler

Mutation Rate: 0.01%

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Last Updated: 06/28/2007