TH01

Other Names
Chromosomal Location
GenBank Accession

HUMTH01, TC11

UniSTS: 240639

11p15.5; intron 1 of human tyrosine hydroxylase gene

Chr 11; 2.149 Mb (May 2004, NCBI build 35)

D00269; has 9 repeat units

Repeat: [AATG] = bottom strand (commonly used); [TCAT] = GenBank top strand
 

Reported Primers

Ref.

PCR Primer Sequences

Set 1

1, 3

5'-GTGGGCTGAAAAGCTCCCGATTAT-3' (AATG strand) 

5'-ATTCAAAGGGTATCTGGGCTCTGG-3' (TCAT strand)

Set 2

4

5'-GTGGGCTGAAAAGCTCCCGATTAT-3' (AATG strand)

5'-GTGATTCCCATTGGCCTGTTCCTC-3' (TCAT strand)

Set 3

125

5'-GCTTCCGAGTGCAGGTCACA-3' (AATG strand)

5'-CAGCTGCCCTAGTCAGCAC-3' (TCAT strand)

Set 4

Promega

PowerPlex 1.1 (TMR labeled) primer sequences

5'-ATTCAAAGGGTATCTGGGCTCTGG-3'

5'-[TMR]-GTGGGCTGAAAAGCTCCCGATTAT-3'

Set 5

ABI

COfiler (JOE labeled), SGM Plus (NED labeled), Identifiler (VIC labeled)

Set 6

Promega

PowerPlex 2.1, PowerPlex 16 (FL labeled) primer sequences  

5'-[FL]-GTGATTCCCATTGGCCTGTTC-3'

5'-ATTCCTGTGGGCTGAAAAGCTC-3'

PCR Product Sizes of Observed Alleles
 

Allele

(Repeat #)

Set 1, 4

Set 2

Set 3

Set 5

Set 6

Repeat Structure

Ref.
3 171 bp 146 bp 230 bp 160 bp 152 bp [AATG]3 290
4 175 bp 150 bp 234 bp 164 bp 156 bp [AATG]4 716
5  179 bp  154 bp  238 bp 168 bp 160 bp [AATG]5 76
5.3 182 bp 157 bp 241 bp 171 bp 163 bp   variant allele
6 183 bp 158 bp 242 bp 172 bp 164 bp [AATG]6 76
6.1 184 bp 159 bp 243 bp 173 bp 165 bp   variant allele
6.3 186 bp 161 bp 245 bp 175 bp 167 bp [AATG]3ATG[AATG]3 632
7 187 bp 162 bp 246 bp 176 bp 168 bp [AATG]7 76
7.1 188 bp 163 bp 247 bp 177 bp 169 bp   variant allele
7.3 190 bp 165 bp 249 bp 179 bp 171 bp   variant allele
8 191 bp 166 bp 250 bp 180 bp 172 bp [AATG]8 76
8.3 194 bp 169 bp 253 bp 183 bp 175 bp [AATG]5ATG[AATG]3 76
9 195 bp 170 bp 254 bp 184 bp 176 bp [AATG]9 76
9.1 196 bp 171 bp 255 bp 185 bp 177 bp   variant allele
9.3 198 bp 173 bp 257 bp 187 bp 179 bp [AATG]6ATG[AATG]3 76
10 199 bp 174 bp 258 bp 188 bp 180 bp [AATG]10 76
10.3 202 bp 177 bp 261 bp 191 bp 183 bp [AATG]6ATG[AATG]4 76
11 203 bp 178 bp 262 bp 192 bp 184 bp [AATG]11 76
12 207 bp 182 bp 266 bp 196 bp 188 bp [AATG]12 112
13.3 214 bp 189 bp 273 bp 203 bp 195 bp [AATG][AACT][AATG]8ATG[AATG]3 377
14 215 bp 190 bp 274 bp 204 bp 196 bp   variant allele

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: PowerPlex 1.1, PowerPlex 16, COfiler, SGM Plus, Identifiler

Mutation Rate: 0.01%

 

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Last Updated: 06/28/2007