D21S11

Other Names

Chromosomal Location

GenBank Accession

UniSTS: 240642

  21q21.1

Chr 21; 19.476 Mb (May 2004, NCBI build 35)

M84567; has 26 repeat units

AP000433; has 29.1 repeat units

Repeat: [TCTA], [TCTG]= GenBank top strand
 

Reported Primers

Ref.

PCR Primer Sequences

Set 1

613, 34

5'-GTG AGT CAA TTC CCC AAG-3'

5'-GTT GTA TTA GTC AAT GTT CTC C-3'

Set 2

5

5'-ATA TGT GAG TCA ATT CCC CAA G-3'

5'-TGT ATT AGT CAA TGT TCT CCA G-3'

Set 3

ABI

Profiler Plus (JOE labeled), SGM Plus (JOE labeled), Identifiler (6-FAM labeled)

Set 4

Promega

PowerPlex 2.1, PowerPlex 16 (FL labeled) primer sequences

5'-ATATGTGAGTCAATTCCCCAAG-3'

5'-[FL]-TGTATTAGTCAATGTTCTCCAGAGAC-3'

PCR Product Sizes of Observed Alleles

Allele (Repeat #)

(Ref. # 34)

Allele    (Repeat #)

 

(Ref. # 5)

Set 1,2

Set 3

Set 4

Repeat Structure

Ref.
12   154 bp 138 bp 155 bp   1672

24

53

202 bp

186 bp

203 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]6

716

24.2

54

204 bp 

188 bp

205 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8

312
24.3   205 bp 189 bp 206 bp   variant allele

25

55

206 bp

190 bp

207 bp

[TCTA]4 [TCTG]3 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10

312
25.1   207 bp 191 bp 208 bp   variant allele

25.2

56

208 bp

192 bp

209 bp

[TCTA]5 [TCTG]6 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10

716
25.3   209 bp 193 bp 210 bp   variant allele

26

57

210 bp

194 bp

211 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8

34

26.1

 

211 bp

195 bp

212 bp

 

SGM Plus

26.2

58

212 bp

196 bp

213 bp

 

variant allele

27

59

214 bp

198 bp

215 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]9

34

27'

59

214 bp

198 bp

215 bp

[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8

34

27"

59

214 bp

198 bp

215 bp

[TCTA]5 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]9

716
27.1   215 bp 199 bp 216 bp   variant allele

27.2

60

216 bp

200 bp

217 bp

 

variant allele

27.3

 

217 bp

201 bp

218 bp

 

variant allele

28

61

218 bp

202 bp

219 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10

34

28'

 

218 bp

202 bp

219 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]9

490
28.1   219 bp 203 bp 220 bp   variant allele

28.2

62

220 bp

204 bp

221 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10

716

28.2'

 

220 bp

204 bp

221 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8 TA TCTA

490

28.3

 

221 bp

205 bp

222 bp

 

variant allele

29

63

222 bp

206 bp

223 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11

34

29'

63

222 bp

206 bp

223 bp

[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10

490

29.1

 

223 bp

207 bp

224 bp

 

variant allele

29.1

 

223 bp

207 bp

224 bp

[TCTA]4 [TCTG]6 [TCTA]6 TCA [TCTA]2 TCCA TA [TCTA]11

GenBank AP000433

29.2

64

224 bp

208 bp

225 bp

[TCTA]5 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10 TA TCTA

490

29.3

 

225 bp

209 bp

226 bp

 

variant allele

30

65

226 bp

210 bp

227 bp

[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11

34

30'

65

226 bp

210 bp

227 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11

490

30"

65

226 bp

210 bp

227 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12

312

30"'

65

226 bp

210 bp

227 bp

[TCTA]6 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10

404

30.1

 

227 bp

211 bp

228 bp

 

SGM Plus

30.2

66

228 bp

212 bp

229 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10 TA TCTA

716
30.2' 66 228 bp 212 bp 229 bp [TCTA]5 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11 TA TCTA 312
30.3   229 bp 213 bp 230 bp   variant allele

31

67

230 bp

214 bp

231 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12

716

31'

67

230 bp

214 bp

231 bp

[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12

34

31"

67

230 bp

214 bp

231 bp

[TCTA]6 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11

490

31"'

67

230 bp

214 bp

231 bp

[TCTA]7 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11

312

31.1

 

231 bp

215 bp

232 bp

 

variant allele

31.2

68

232 bp

216 bp

233 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11 TA TCTA

34

31.3

 

233 bp

217 bp

234 bp

 

variant allele

32

69

234 bp

218 bp

235 bp

[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13

34

32'

69

234 bp

218 bp

235 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13

490

32.1

 

235 bp

219 bp

236 bp

 

variant allele

32.2

70

236 bp

220 bp

237 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 TA TCTA

34

32.2'

70

236 bp

220 bp

237 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 TA TCTA

404

32.2"

70

236 bp

220 bp

237 bp

[TCTA]5 [TCTG]6 [TCTA]2 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 TA TCTA

404

32.3

 

237 bp

221 bp

238 bp

 

variant allele

33

71

238 bp

222 bp

239 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]14

490
33.1   239 bp 223 bp 240 bp   variant allele

33.2

72

240 bp

224 bp

241 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 TA TCTA

34

33.2'

72

240 bp

224 bp

241 bp

[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 TA TCTA

404

33.2"

72

240 bp

224 bp

241 bp

[TCTA]6 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 TA TCTA

404

33.3

 

241 bp

225 bp

242 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8 TCA [TCTA]2 TA TCTA

404

34

73

242 bp

226 bp

243 bp

[TCTA]10 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11 

404

34'

73

242 bp

226 bp

243 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]15 

490
34.1   243 bp 227 bp 244 bp   variant allele

34.2

74

244 bp

228 bp

245 bp

[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]14 TA TCTA

34

34.3

 

245 bp

229 bp

246 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10 TCA [TCTA]4TA TCTA

404

35

75

246 bp

230 bp

247 bp

[TCTA]11 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11

404

35'

75

246 bp

230 bp

247 bp

[TCTA]10 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12

716

35.1

 

247 bp

231 bp

248 bp

 

variant allele

35.2

76

248 bp

232 bp

249 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]15 TA TCTA

490

35.3

 

249 bp

233 bp

250 bp

 

variant allele

36

77

250 bp

234 bp

251 bp

[TCTA]10 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 

404

36'

77

250 bp

234 bp

251 bp

[TCTA]11 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12

716

36"

77

250 bp

234 bp

251 bp

[TCTA]10 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12

404
36.1   251 bp 235 bp 252 bp   variant allele

36.2

78

252 bp

236 bp

253 bp

[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]16 TA TCTA

490

36.3

 

253 bp

237 bp

254 bp

 

variant allele

37

79

254 bp

238 bp

255 bp

[TCTA]9 [TCTG]11 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12

404

37'

79

254 bp

238 bp

255 bp

[TCTA]11 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13

716

37.2

80

256 bp

240 bp

257 bp

[TCTA]7 [TCTG]14 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12

1926
37.2' 80

256 bp

240 bp

257 bp

 [TCTA]9 [TCTG]12 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 1926
37.2'' 80

256 bp

240 bp

257 bp

 [TCTA]9 [TCTG]13 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11 1926
37.2''' 80

256 bp

240 bp

257 bp

 [TCTA]10 [TCTG]11 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 1926
37.2'''' 80

256 bp

240 bp

257 bp

 [TCTA]11 [TCTG]11 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11 1926

38

81

258 bp

242 bp

259 bp

[TCTA]9 [TCTG]11 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 

404

38'

81

258 bp

242 bp

259 bp

[TCTA]13 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12

716

38"

81

258 bp

242 bp

259 bp

[TCTA]10 [TCTG]11 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 

404

38"'

81

258 bp

242 bp

259 bp

[TCTA]11 [TCTG]11 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11 

404

38.2

82

260 bp

244 bp

261 bp

[TCTA]9 [TCTG]12 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13

1926
38.2' 82 260 bp 244 bp 261 bp [TCTA]9 [TCTG]13 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 1926
38.2'' 82 260 bp 244 bp 261 bp [TCTA]10 [TCTG]11 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 1926
39 83 262 bp 246 bp 263 bp   variant allele
39.2 84 264 bp 248 bp 265 bp [TCTA]10 [TCTG]13 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 1926
39.2' 84 264 bp 248 bp 265 bp [TCTA]11 [TCTG]12 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 1926
40.2 86 268 bp 252 bp 269 bp   1672
41.2 88 272 bp 256 bp 273 bp [TCTA]10 [TCTG]15 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 1926

Explanation of the various allele nomenclatures

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: PowerPlex 2.1, PowerPlex 16, Profiler Plus, SGM Plus, Identifiler

Mutation Rate: 0.19%

 

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Last Updated: 08/01/2012