D5S818

Other Names
Chromosomal Location
GenBank Accession

D5

UniSTS: 54700

5q23.2

Chr 5; 123.139 Mb (May 2004, NCBI build 35)

G08446; has 11 repeat units
AC008512; has 11 repeat units

Repeat: [AGAT] = GenBank top strand
 

Reported Primers

Ref.

PCR Primer Sequences

Set 1

Promega

PowerPlex 1.1 (FL labeled), PowerPlex 16 (JOE labeled) primer sequences

5'-GGTGATTTTCCTCTTTGGTATCC-3'

5'-[JOE]-AGCCACAGTTTACAACATTTGTATCT-3'

Set 2

453

5'-GGGTGATTTTCCTCTTTGGT-3'

5'-TGATTCCAATCATAGCCACA-3' 

Set 3

ABI

 Profiler Plus (NED labeled), Identifiler (PET labeled)

PCR Product Sizes of Observed Alleles
 

Allele

(Repeat #)

Set 1

Set 2

Set 3

Repeat Structure

Ref.
6 115 bp 129 bp 130 bp   variant allele
7 119 bp 133 bp 134 bp [AGAT]7 721
8 123 bp  137 bp  138 bp [AGAT]8 721
9 127 bp 141 bp 142 bp [AGAT]9 721
10 131 bp 145 bp 146 bp [AGAT]10 721
10.1 132 bp 146 bp 147 bp   variant allele
11 135 bp 149 bp 150 bp [AGAT]11 721
11.1 136 bp 150 bp 151 bp   variant allele
12 139 bp 153 bp 154 bp [AGAT]12 721
12.3 142 bp 156 bp 157 bp   variant allele
13 143 bp 157 bp 158 bp [AGAT]13 721
14 147 bp 161 bp 162 bp [AGAT]14 721
15 151 bp 165 bp 166 bp [AGAT]15 721
16 155 bp 169 bp 170 bp  

Profiler Plus
17 159 bp 173 bp 174 bp   variant allele
18 163 bp 177 bp 178 bp   variant allele

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: PowerPlex 1.1, PowerPlex 16, Profiler Plus, Identifiler

Mutation Rate: 0.11%

 

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Last Updated: 06/28/2007