CSF1PO

Other Names
Chromosomal Location
GenBank Accession

CSF

UniSTS: 156169

5q33.1; human c-fms proto-oncogene for CSF-1 receptor gene, 6th intron

Chr 5; 149.436 Mb (May 2004, NCBI build 35)

X14720; has 12 repeats

Repeat: [AGAT] = GenBank top strand (commonly used)
 

Reported Primers Ref. PCR Primer Sequences

Set 1

3

5'-AACCTGAGTCTGCCAAGGACTAGC-3' (AGAT strand)

5'-TTCCACACACCACTGGCCATCTTC-3' (TCTA strand)

Set 2

Promega

PowerPlex 1.1 (TMR labeled)

Set 3

ABI

COfiler (JOE labeled)

Set 4

Promega

PowerPlex 16 (JOE labeled) primer sequences

5'-[JOE]-CCGGAGGTAAAGGTGTCTTAAAGT-3'

5'-ATTTCCTGTGTCAGACCCTGTT-3'
Set 5 ABI Identifiler (6FAM labeled with *mobility modifier)

PCR Product Sizes of Observed Alleles
 

Allele 

(Repeat #)
Set 1 &

Set 2
Set 3
 Set 4 Set 5*
Repeat Structure
Ref.
5 287 bp 277 bp 317 bp 301 bp [AGAT]5 variant allele
6
291 bp
281 bp
 321 bp 305 bp [AGAT]6 563
6.3 294 bp 284 bp 324 bp 308 bp   variant allele
7
295 bp
285 bp
 325 bp 309 bp [AGAT]7 153
7.3 298 bp 288 bp 328 bp 312 bp   variant allele
8
299 bp
289 bp
 329 bp 313 bp [AGAT]8 28
8.3 302 bp 292 bp 332 bp 316 bp   variant allele
9
303 bp
293 bp
 333 bp 317 bp [AGAT]9 28
9.1 304 bp 294 bp 334 bp 318 bp   variant allele
9.3 306 bp 296 bp 336 bp 320 bp   variant allele
10
307 bp
297 bp
 337 bp 321 bp [AGAT]10 28
10.1 308 bp 298 bp 338 bp 322 bp   variant allele
10.2 309 bp 299 bp 339 bp 323 bp   variant allele
10.3
310 bp
300 bp
 340 bp 324 bp   563
11
311 bp
301 bp
 341 bp 325 bp [AGAT]11 28
11.1 312 bp 302 bp 342 bp 326 bp   variant allele
11.3 314 bp 304 bp 344 bp 328 bp   variant allele
12
315 bp
305 bp
 345 bp 329 bp [AGAT]12 28
12.1 316 bp 306 bp 346 bp 330 bp   variant allele
13
319 bp
309 bp
 349 bp 333 bp [AGAT]13 28
14
323 bp
313 bp
 353 bp 337 bp [AGAT]14 563
15
327 bp
317 bp
 357 bp 341 bp [AGAT]15 563
16 331bp 321 bp 361 bp 345 bp [AGAT]16 variant allele

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: PowerPlex 1.1, PowerPlex 16, COfiler, Identifiler

Mutation Rate: 0.16%

 

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Last Updated: 03/11/2013