D3S1358

Other Names
Chromosomal Location
GenBank Accession

UniSTS: 148226

3p21.31

Chr 3; 45.557 Mb (May 2004, NCBI build 35)

AC099539 has 16 repeats

Repeat: [AGAT], [TCTA]= bottom strand
 

Reported Primers Ref. PCR Primer Sequences

Set 1

148, 502

5'-ACT GCA GTC CAA TCT GGG T-3' (AGAT strand)

5'-ATG AAA TCA ACA GAG GCT TG-3' (TCTA strand)

Set 2

ABI

Profiler Plus, COfiler, SGM Plus, Identifiler

Set 3 

Promega

PowerPlex 2.1, PowerPlex 16 (FL labeled) primer sequences

5'-ACTGCAGTCCAATCTGGGT-3'

5'-[FL]-ATGAAATCAACAGAGGCTTGC-3'

PCR Product Sizes of Observed Alleles
 

Allele       (Repeat #)
Set 1,3
Set 2
Repeat Structure
 Ref.
8 99 bp 97 bp  

variant allele
8.3 102 bp 100 bp   variant allele
9 103 bp 101 bp   variant allele
10 107 bp 105 bp   variant allele
11 111 bp 109 bp   variant allele
12 115 bp 113 bp   SGM Plus
13 119 bp  117 bp  TCTA[TCTG]2[TCTA]10 729
14 123 bp 121 bp TCTA[TCTG]2[TCTA]11 668
14.3 126 bp 124 bp   variant allele
15 127 bp 125 bp TCTA[TCTG]3[TCTA]11 668
15' 127 bp 125 bp TCTA[TCTG]2[TCTA]12 668
15.1 128 bp 126 bp   variant allele
15.2 129 bp 127 bp   variant allele
15.3 130 bp 128 bp   variant allele
16 131 bp 129 bp TCTA[TCTG]3[TCTA]12 668
16' 131 bp 129 bp TCTA[TCTG]2[TCTA]13 729
16.2 133 bp 131 bp   642
17 135 bp 133 bp TCTA[TCTG]3[TCTA]13 668
17' 135 bp 133 bp TCTA[TCTG]2[TCTA]14 729
17.1 136 bp 134 bp   variant allele
17.2 137 bp 135 bp   variant allele
18 139 bp 137 bp TCTA[TCTG]3[TCTA]14 668
18.1 140 bp 138 bp   variant allele
18.2 141 bp 139 bp   variant allele
18.3 142 bp 140 bp  

variant allele
19 143 bp 141 bp TCTA[TCTG]3[TCTA]15 729
20  147 bp 145 bp   729

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: PowerPlex 2.1, PowerPlex 16, Profiler Plus, COfiler, SGM Plus, Identifiler

Mutation Rate: 0.12%

 

RETURN TO STR Fact Sheet Page

RETURN TO HOMEPAGE

Last Updated: 06/28/2007