D18S51

Other Names
Chromosomal Location
GenBank Accession

D18S379; UT574

UniSTS: 44409

18q21.33

Chr 18; 59.100 Mb (May 2004, NCBI build 35)

X91254; has 21 repeats
AP001534; has 18 repeats

Repeat: [GAAA] = GenBank top strand
 

Reported Primers

Ref.

PCR Primer Sequences

Set 1

7, 369

5'-CAA ACC CGA CTA CCA GCA AC-3' (GAAA strand)

5'-GAG CCA TGT TCA TGC CAC TG-3' 

Set 2

ABI

Profiler Plus (JOE labeled), SGM Plus (JOE labeled), Identifiler (NED labeled)

Set 3

Promega

PowerPlex 2.1 (FL labeled), PowerPlex 16 (FL labeled) primer sequences

5'-[FL]-TTCTTGAGCCCAGAAGGTTA-3'

5'-ATTCTACCAGCAACAACACAAATAAAC-3'

PCR Product Sizes of Observed Alleles
 

Allele (Repeat #)

Set 1

Set 2

Set 3

Repeat Structure
3' Flanking Sequence 
Ref.
7 262 bp 264 bp 286 bp     variant allele
8 266 bp 268 bp 290 bp [AGAA]8 AAAG AGAGAG 716
9 270 bp 272 bp 294 bp [AGAA]9 AAAG AGAGAG 716
9.2 272 bp 274 bp 296 bp     SGM Plus
10 274 bp  276 bp 298 bp [AGAA]10 AAAG AGAGAG 716
10.2 276 bp 278 bp 300 bp     SGM Plus
11 278 bp 280 bp 302 bp [AGAA]11 AAAG AGAGAG 716
11.1 279 bp 281 bp 303 bp     variant allele
11.2 280 bp 282 bp 304 bp     variant allele
12 282 bp 284 bp 306 bp [AGAA]12 AAAG AGAGAG 716
12.2 284 bp 286 bp 308 bp     variant allele
12.3 285 bp 287 bp 309 bp     variant allele
13 286 bp 288 bp 310 bp [AGAA]13 AAAG AGAGAG 716
13.1 287 bp 289 bp 311 bp    

variant allele
13.2 288 bp 290 bp 312 bp [AGAA]13 AGAG AG AGAGAG 369
13.3 289 bp 291 bp 313 bp     variant allele
14 290 bp 292 bp 314 bp [AGAA]14 AAAG AGAGAG 716
14.2 292 bp 294 bp 316 bp [AGAA]14 AGAG AG AGAGAG 369
15 294 bp 296 bp 318 bp [AGAA]15 AAAG AGAGAG 716
15.1 295 bp 297 bp 319 bp     variant allele
15.2 296 bp 298 bp 320 bp [AGAA]15 AGAG AG AGAGAG 369
15.3 297 bp 299 bp 321 bp     variant allele
16 298 bp 300 bp 322 bp [AGAA]16 AAAG AGAGAG 716
16.1 299 bp 301 bp 323 bp     variant allele
16.2 300 bp 302 bp 324 bp     variant allele
16.3 301 bp 303 bp 325 bp     variant allele
17 302 bp 304 bp 326 bp [AGAA]17 AAAG AGAGAG 716
17.1 303 bp 305 bp 327 bp     SGM Plus
17.2 304 bp 306 bp 328 bp [AGAA]17 AAAG AGAGAG 375
17.3 305 bp 307 bp 329 bp     variant allele
18 306 bp 308 bp 330 bp [AGAA]18 AAAG AGAGAG 716
18.1 307 bp 309 bp 331 bp     variant allele
18.2 308 bp 310 bp 332 bp     variant allele
19 310 bp 312 bp 334 bp [AGAA]19 AAAG AGAGAG 716
19.2 312 bp 314 bp 336 bp [AGAA]19 AAAG AGAGAG 375
20 314 bp 316 bp 338 bp [AGAA]20 AAAG AGAGAG 716
20.1 315 bp 317 bp 339 bp     variant allele
20.2 316 bp 318 bp 340 bp     variant allele
21 318 bp 320 bp 342 bp [AGAA]21 AAAG AGAGAG 716
21.1 319 bp 321 bp 343 bp     variant allele
21.2 320 bp 322 bp 344 bp     variant allele
22 322 bp 324 bp 346 bp [AGAA]22 AAAG AGAGAG 716
22.1 323 bp 325 bp 347 bp    

variant allele
22.2 324 bp 326 bp 348 bp     variant allele
23 326 bp 328 bp 350 bp [AGAA]23 AAAG AGAGAG 716
23.1 327 bp 329 bp 351 bp     SGM Plus
23.2 328 bp 330 bp 352 bp     variant allele
24 330 bp  332 bp 354 bp [AGAA]24 AAAG AGAGAG 716
24.2 332 bp 334 bp 356 bp     variant allele
25 334 bp 336 bp 358 bp [AGAA]25 AAAG AGAGAG 716
26 338 bp  340 bp 362 bp [AGAA]26 AAAG AGAGAG 716
27 342 bp 344 bp 366 bp [AGAA]27 AAAG AGAGAG 716
28.1 347 bp 349 bp 371 bp     variant allele
28.3 349 bp 351 bp 373 bp     variant allele
39.2 (40?)   396.37 bp       variant allele

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: PowerPlex 16, PowerPlex 2.1, Profiler Plus, SGM Plus, Identifiler

Mutation Rate: 0.22%

 

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Last Updated: 10/30/2015