STR Fact Sheet--VWA

VWA

Other Names	Chromosomal Location	GenBank Accession
vWF, VWA31A UniSTS: 240640	12p13.31; von Willebrand Factor, 40^th intron Chr 12; 5.963 Mb (May 2004, NCBI build 35)	M25858; has 18 repeat units

Repeat: [AGAT] = bottom strand (commonly used); [TCTA] with [TCTG] and [TCCA] inserts = GenBank top strand

Reported Primers	Ref.	PCR Primer Sequences
Set 1	113	5'-CCCTAGTGGATAAGAATAATC-3' 5'-GGACAGATGATAAATACATAGGATGGATGG-3'
Set 1'	7	5'-CCCTAGTGGATGATAAGAATAATCAGTATG-3' 5'-GGACAGATGATAAATACATAGGATGGATGG-3'
Set 2	Promega	PowerPlex 1.1, PowerPlex 2.1, PowerPlex 16 (TMR labeled) primer sequences 5'-GCCCTAGTGGATGATAAGAATAATCAGTATGTG-3' 5'-[TMR]-GGACAGATGATAAATACATAGGATGGATGG-3'
Set 3	ABI	Profiler Plus (5-FAM labeled), SGM Plus (5-FAM labeled), Identifiler (NED labeled)

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1, 1'	Set 2	Set 3	Repeat Structure	Ref.
10	122 bp	123 bp	152 bp	TCTA TCTG TCTA [TCTG]₄[TCTA]₃	716
11 (13')*	126 bp	127 bp	156 bp	TCTA[TCTG]₃[TCTA]₇	76
12	130 bp	131 bp	160 bp	TCTA[TCTG]₄[TCTA]₇	7
13	134 bp	135 bp	164 bp	[TCTA]₂[TCTG]₄[TCTA]₃TCCA[TCTA]₃	716
13 (15)	134 bp	135 bp	164 bp	TCTA[TCTG]₄[TCTA]₈TCCA TCTA	76
13 (15'')	134 bp	135 bp	164 bp	TCTA[TCTG]₄[TCTA]₁₀	76
14 (16'')	138 bp	139 bp	168 bp	TCTA[TCTG]₄[TCTA]₁₁	76
14' (16''')	138 bp	139 bp	168 bp	TCTA TCTG[TCTA]₁[TCTG]₄[TCTA]₃TCCA [TCTA]₃	76
15 (17)	142 bp	143 bp	172 bp	TCTA[TCTG]₄[TCTA]₁₀TCCA TCTA	76
15 (17')	142 bp	143 bp	172 bp	TCTA[TCTG]₃[TCTA]₁₁TCCA TCTA	76
15.2	144 bp	145 bp	174 bp		7
16 (18)	146 bp	147 bp	176 bp	TCTA[TCTG]₄[TCTA]₁₁TCCA TCTA	76
16 (18')	146 bp	147 bp	176 bp	TCTA[TCTG]₃[TCTA]₁₂TCCA TCTA	76
16.1	147 bp	148 bp	177 bp		variant allele
17 (19)	150 bp	151 bp	180 bp	TCTA[TCTG]₄[TCTA]₁₂TCCA TCTA	76
18 (20)	154 bp	155 bp	184 bp	TCTA[TCTG]₄[TCTA]₁₃TCCA TCTA	76
18' (20')	154 bp	155 bp	184 bp	TCTA[TCTG]₅[TCTA]₁₂TCCA TCTA	76
18.1				TCTA[TCTG]₄[TCTA]₁₂A TCTA TCCA TCTA	1944
18.2	156 bp	157 bp	186 bp		variant allele
18.3	157 bp	158 bp	187 bp		variant allele
19 (21)	158 bp	159 bp	188 bp	TCTA[TCTG]₄[TCTA]₁₄TCCA TCTA	76
19.2					variant allele
20 (22)	162 bp	163 bp	192 bp	TCTA[TCTG]₄[TCTA]₁₅TCCA TCTA	76
21 (23)	166 bp	167 bp	196 bp	TCTA[TCTG]₄[TCTA]₁₆TCCA TCTA	76
22 (24)	170 bp	171 bp	200 bp	TCTA[TCTG]₄[TCTA]₁₇TCCA TCTA	76
23	174 bp	175 bp	204 bp		variant allele
24	178 bp	179 bp	208 bp		variant allele
25	182 bp	183 bp	212 bp		variant allele

*Allele designations shown in parentheses come from reference 76.

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: PowerPlex 1.1, PowerPlex 2.1, PowerPlex 16, Profiler Plus, SGM Plus, Identifiler

Mutation Rate: 0.17%

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Last Updated: 09/23/2010