FGA

Other Names

Chromosomal Location

GenBank Accession

FIBRA

UniSTS: 240635

4q28; located in the third intron of the human alpha fibrinogen gene

Chr 4; 155.866 Mb (May 2004, NCBI build 35)

M64982; has 21 repeats

Repeat: complex tetranucleotide repeat; [TTTC]3TTTTTTCT[CTTT]nCTCC[TTCC]2 = GenBank top strand

Reported Primers

Ref.

PCR Primer Sequences

Set 1

7

5'-GCCCCATAGGTTTTGAACTCA-3' (CTTT strand)

5'-TGATTTGTCTGTAATTGCCAGC-3' (GAAA strand)

Set 2

363

AmpFlSTR Profiler Plus (5-FAM labeled), SGM Plus (NED labeled), Identifiler (PET labeled)

Set 3

Promega

PowerPlex 16 (TMR labeled) primer sequences 

5'-[TMR]-GGCTGCAGGGCATAACATTA-3'

5'-ATTCTATGACTTTGCGCTTCAGGA-3'

PCR Product Sizes of Observed Alleles

Allele

(Repeat #)

Set 1

Set 2

Set 3

Repeat Structure

Ref.
12.2 158 bp 196 bp 308 bp   variant allele
13 160 bp 198 bp 310 bp   variant allele 
13.2 162 bp 200 bp 312 bp   variant allele
14 164 bp 202 bp 314 bp   variant allele
14.3 167 bp 205 bp 317 bp   variant allele

15

168 bp

206 bp

 318 bp

[TTTC]3TTTT TTCT[CTTT]7CTCC[TTCC]2

142
15.3 171 bp 209 bp 321 bp   variant allele

16

 172 bp

210 bp

 322 bp

[TTTC]3TTTTTTCT[CTTT]8CTCC[TTCC]2

363
16.1 173 bp 211 bp 323 bp [TTTC]3TTTTTTCT[CTTT]5T[CTTT]3CTCC[TTCC]2 716

16.2

 174 bp

212 bp

 324 bp

[TTTC]3TTTT TT [CTTT]9CTCC[TTCC]2

363

17

176 bp

214 bp

 326 bp

[TTTC]3TTTT TTCT[CTTT]9CTCC[TTCC]2

142
17.1 177 bp 215 bp 327 bp   variant allele
17.2 178 bp 216 bp 328 bp   variant allele

18

180 bp

218 bp

 330 bp

[TTTC]3TTTT TTCT[CTTT]10CTCC[TTCC]2

142
18.1 181 bp 219 bp 331 bp   variant allele

18.2

182 bp

220 bp

 332 bp

[TTTC]3TTTT TT [CTTT]11CTCC[TTCC]2

142

19

184 bp

222 bp

 334 bp

[TTTC]3TTTT TTCT[CTTT]11CTCC[TTCC]2

142
19.1 185 bp 223 bp 335 bp   variant allele

19.2

 186 bp

224 bp

 336 bp

[TTTC]3TTTT TT [CTTT]12CTCC[TTCC]2

363
19.3 187 bp 225 bp 337 bp   variant allele

20

188 bp

226 bp

 338 bp

[TTTC]3TTTT TTCT[CTTT]12CTCC[TTCC]2

142
20.1 189 bp 227 bp 339 bp   variant allele

20.2

190 bp

228 bp

 340 bp

[TTTC]3TTTT TT [CTTT]13CTCC[TTCC]2

142
20.3 191 bp 229 bp 341 bp   variant allele

21

192 bp

230 bp

 342 bp

[TTTC]3TTTT TTCT[CTTT]13CTCC[TTCC]2

142
21.1 193 bp 231 bp 343 bp   variant allele

21.2

 194 bp

232 bp

 344 bp

[TTTC]3TTTT TT [CTTT]14CTCC[TTCC]2

363
21.3 195 bp 233 bp 345 bp   variant allele

22

196 bp

234 bp

 346 bp

[TTTC]3TTTT TTCT[CTTT]14CTCC[TTCC]2

142

22.1

197 bp 235 bp 347 bp   variant allele

22.2

198 bp

236 bp

 348 bp

[TTTC]3TTTT TT [CTTT]15CTCC[TTCC]2

142

22.3

 199 bp

237 bp

 349 bp  

variant allele

23

200 bp

238 bp

 350 bp

[TTTC]3TTTT TTCT[CTTT]15CTCC[TTCC]2

142

23.1

 201 bp 239 bp 351 bp   variant allele

23.2

202 bp

240 bp

 352 bp

[TTTC]3TTTT TT [CTTT]16CTCC[TTCC]2

142
23.3 203 bp 241 bp 353 bp   variant allele

24

204 bp

242 bp

 354 bp

[TTTC]3TTTT TTCT[CTTT]16CTCC[TTCC]2

142
24.1 205 bp 243 bp 355 bp   variant allele

24.2

206 bp

244 bp

 356 bp

[TTTC]3TTTT TT [CTTT]17CTCC[TTCC]2

142
24.3 207 bp 245 bp 357 bp   variant allele

25

208 bp

246 bp

 358 bp

[TTTC]3TTTT TTCT[CTTT]17CTCC[TTCC]2

142
25.1 209 bp 247 bp 359 bp   variant allele 

25.2

 210 bp

248 bp

 360 bp

[TTTC]3TTTT TT [CTTT]18CTCC[TTCC]2

363
25.3 211 bp 249 bp 361 bp   variant allele

26

212 bp

250 bp

 362 bp

[TTTC]3TTTT TTCT[CTTT]18CTCC[TTCC]2

142
26.1 213 bp 251 bp 363 bp   variant allele

26.2

 214 bp

252 bp

 364 bp

[TTTC]3TTTT TT [CTTT]19CTCC[TTCC]2

363
26.3 215 bp 253 bp 365 bp   variant allele

27

216 bp

254 bp

 366 bp

[TTTC]3TTTT TTCT[CTTT]19CTCC[TTCC]2

142
27' 216 bp 254 bp 366 bp

[TTTC]3TTTT TTCT[CTTT]13CCTT[CTTT]5CTCC[TTCC]2

 716
27.1 217 bp 255 bp 367 bp   variant allele

27.2

 218 bp

256 bp

 368 bp

[TTTC]3TTTT TT [CTTT]20CTCC[TTCC]2

363
27.3 219 bp 257 bp 369 bp   variant allele

28

220 bp

258 bp

 370 bp

[TTTC]3TTTT TTCT[CTTT]20CTCC[TTCC]2

142
28.1 221 bp 259 bp 371 bp   variant allele 

28.2

 222 bp

260 bp

 372 bp

[TTTC]3TTTT TT [CTTT]21CTCC[TTCC]2

363

29

224 bp

262 bp

 374 bp

[TTTC]3TTTT TTCT[CTTT]15CCTT[CTTT]5CTCC[TTCC]2

142
29.1 225 bp 263 bp 375 bp   variant allele
29.2 226 bp 264 bp 376 bp   variant allele

30

 228 bp

266 bp

 378 bp

[TTTC]3TTTT TTCT[CTTT]16CCTT[CTTT]5CTCC[TTCC]2

363

30.2

230 bp

268 bp

 380 bp

[TTTC]4TTTT TT [CTTT]14[CTTC]3[CTTT]3 CTCC [TTCC]4

142
31 232 bp 270 bp 382 bp   variant allele
31.2 234 bp 272 bp 384 bp

[TTTC]4TTTT TT [CTTT]15[CTTC]3[CTTT]3 CTCC [TTCC]4

 716
32 236 bp 274 bp 386 bp   variant allele
32.1 237 bp 275 bp 387 bp   variant allele
32.2 238 bp 276 bp 388 bp

[TTTC]4TTTT TT [CTTT]16[CTTC]3[CTTT]3 CTCC [TTCC]4

 716
33.1 241 bp 279 bp 391 bp   variant allele
33.2 242 bp 280 bp 392 bp

[TTTC]4TTTT TT [CTTT]17[CTTC]3[CTTT]3 CTCC [TTCC]4

 716
34.1 245 bp 283 bp 395 bp   variant allele

34.2

246 bp

284 bp

 396 bp

[TTTC]4TTTT TT [CTTT]18[CTTC]3[CTTT]3 CTCC[TTCC]4

142
35.2 250 bp 288 bp 400 bp   variant allele
41.1 273 bp 311 bp 423 bp   variant allele
41.2 274 bp 312 bp 424 bp   variant allele
42 276 bp 314 bp 426 bp   variant allele
42.1 277 bp 315 bp 427 bp   variant allele
42.2 278 bp 316 bp 428 bp

[TTTC]4TTTT TT [CTTT]8[CTGT]4[CTTT]13[CTTC]4[CTTT]3 CTCC [TTCC]4

 716
43.1 281 bp 319 bp 431 bp   variant allele
43.2 282 bp 320 bp 432 bp [TTTC]4TTTT TT [CTTT]8 [CTGT]5[CTTT]13[CTTC]4[CTTT]3CTCC[TTCC]4 716
44 284 bp 322 bp 434 bp   variant allele
44.2 286 bp 324 bp 436 bp [TTTC]4TTTT TT     [CTTT]11[CTGT]3[CTTT]14 [CTTC]3[CTTT]3CTCC[TTCC]4 716
44.3 287 bp 325 bp 437 bp   variant allele
45 288 bp 326 bp 438 bp   variant allele
45.1 289 bp 327 bp 439 bp   variant allele
45.2 290 bp 328 bp 440 bp [TTTC]4TTTT TT     [CTTT]10[CTGT]5[CTTT]13 [CTTC]4[CTTT]3CTCC[TTCC]4 716
46 292 bp 330 bp 442 bp   variant allele
46.1 293 bp 331 bp 443 bp   variant allele

46.2

294 bp

332 bp

 444 bp

[TTTC]4TTTTTT [CTTT]12[CTGT]5[CTTT]13 [CTTC]3[CTTT]3CTCC[TTCC]4

142
47 296 bp 334 bp 446 bp   variant allele
47.2 298 bp 336 bp 448 bp [TTTC]4TTTT TT [CTTT]12[CTGT]5[CTTT]14 [CTTC]3[CTTT]3CTCC[TTCC]4 716
48 300 bp 338 bp 450 bp   variant allele
48.2 302 bp 340 bp 452 bp [TTTC]4TTTT TT [CTTT]14[CTGT]3[CTTT]14 [CTTC]4[CTTT]3CTCC[TTCC]4 716
49 304 bp 342 bp 454 bp   variant allele
49.1 305 bp 343 bp 455 bp   variant allele
49.2 306 bp 344 bp 456 bp   variant allele
50.2 310 bp 348 bp 460 bp [TTTC]4TTTT TT     [CTTT]14[CTGT]4[CTTT]15 [CTTC]4[CTTT]3CTCC[TTCC]4 716
50.3 311 bp 349 bp 461 bp   variant allele
51 312 bp 350 bp 462 bp   variant allele
51.2 314 bp 352 bp 464 bp   variant allele

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: Profiler Plus, SGM Plus, Identifiler, PowerPlex 2.1, PowerPlex 16

Mutation Rate: 0.28%

 

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Last Updated: 05/15/2015