TPOX Variants
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|
Allele Designation |
Allele Size |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
|
4 |
207.18 |
ABI 310 |
CO |
Re-extracted and re-amplified |
|
|
|
| 4 [2] | 214.05 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville, TN | Re-extracted observed in multiple samples | ||
| 4 [3] | 213.89 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville, TN | Re-extracted observed in multiple samples | ||
| 4 [4] |
252.93 253.08 |
ABI 3130 ABI 3130xl |
PP | Candace Jones, North Louisiana Criminalistics Laboratory | Re-amplified | 2 | |
| 4 (<6) | 214.06-214.26 | ABI 3130 XL | ID | Marie-Beatrice Tonanny & Frederic G. Brard, RCGN-National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois,France | re-extraction and re-amplification | FTA card - saliva | 2 in 154,000 |
| <5 | 204.74 | ABI 3730 | ID, PP16HS | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-amplified and re-analyzed with ABI 3130 | 1 in 31,330 | |
|
5 |
212.4 |
ABI 310 |
PR, CV |
Re-amplified with two kits |
|
1 in 600 samples |
|
|
5 [2] |
214.21 |
ABI 377 |
CO |
|
Re-amplified |
Convicted offender known |
1 |
| 5 [3] | 218.07 - 218.09 | ABI 310 | ID | Andrea Verzeletti, Istituto de Medicina Legale, Universita degli Studi di Brescia, Brescia, Italia | Re-extracted and Re-amplified | Paternity testing case | |
|
5 (<6) |
210.96 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
|
5 (<6) [2] |
210.91 |
ABI 3100 |
CO |
Nicole Hahn M U F S C |
Re-amplified for confirmation |
|
1 |
|
5 (<6) [3] |
211.04 |
ABI 3100 |
CO |
Nicole Hahn M U F S C |
Previously Seen - No verification |
|
2 |
|
5 (<6) [4] |
211 | ABI 3100 | CO |
Nicole Hahn M U F S C |
|||
| 5 (<6) [5] | 211.14 | ABI 3100 | CO | Nicole Hoffman M U F S C | Previously Seen - No verification | 3 | |
| 5 (<6) [6] | 217.85 217.89 | ABI 3130XL | ID | Laurent Cardin & Frederic G. Brard, IRCGN-National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois,France | re-extraction and re-amplification | FTA card - saliva | 2 in 25000 |
| 7.1 | 227.05 | ABI 310 | ID | Janel Smith, Phoenix Police Department | Present in both exemplar and seven intimate evidence samples | ||
| 7.1 [2] | 226.82 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-extracted | ||
| 7.2 | 227.6 | ABI 3100 | ID |
Marsha Garcia, Orchid Cellmark, Nashville TN
|
Observed in multiple samples | ||
|
7.3 |
225.67 |
ABI 377 |
PS |
Catherine Allor |
Re-amplified and Reanalyzed |
Paternity samples only |
1 in 33300 |
| 7.3 [2] | 228.69 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | |||
|
10.1 |
278.08 |
ABI 310 |
PP |
Jackie Johnson, MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
|
| 10.1 [2] | 238.51-238.64 | ABI 3130XL | ID | Laurent Cardin and Frederic G. Brard, IRCGN, France | re-extraction and re-amplification | FTA card - saliva/POP4 | 1/50.000 |
|
10.3 |
|
|
PP & PP 2.1 |
MD Sate Police |
Re-extracted, re-amplified, re-run and sequenced |
Sample is actually 1 bp del. 157 bases from the repeat. |
|
|
11 |
|
|
PP1.1 & ID |
MD Sate Police |
Re-extracted, re-amplified, re-run and sequenced |
Sample is actually 1 bp del. 157 bases from the repeat |
|
| 12.1 | 146.42 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-direct amplification | Convicted offender | |
| 12.3 | 248.74 | ABI 3100, ABI 3130 | ID | Park Hee-Yeon, NFS Southern District, Gyeongnam, South Korea | Re-amplification | PP16 - no peak, casework | |
|
13.1 |
250.96 |
ABI 377 |
CO |
Present in both exemplar and evidence samples |
|
|
|
| >13 (14) | 246.79 | AI 3100 | CO | Nicole Hahn M U F S C | |||
|
>13 |
257.48 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amplification |
|
1 in 11,512 |
|
14 |
250.77 |
ABI 377 |
CO, PS |
Re-extracted and re-amplified |
|
|
|
|
14 [2] |
250.93 |
ABI 377 |
CO |
|
Re-amplified |
Convicted offender known |
1 |
| 14 [3] | 246.79 | ABI 3100 | CO | KB M U F S C | |||
| 14 [4] | 253.67 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-extracted | ||
| 14 [5] | 253.99 | ABI 3130xl | ID | Shannon Soltysiak, NYC OCME Dept of Forensic Biology | Re-amplified | ||
| 14 [6] | 434.99 | ABI 3500 | PP21 | François Chatenay-Lapointe, Rhode Island Blood Center | Re-amplified and Re-analyzed | Immigration case. | 2 in 3874 |
| 14 (>13) | 246.78 | ABI 3100 | CO |
Nicole Hahn M U F S C |
Previously Seen – no verification |
2 | |
| 14 (>13) [2] | 246.95 | ABI 3100 | CO | Nicole Hoffman M U F S C | |||
|
15 (>13) |
297.01 |
ABI 310 |
PP |
Jackie Johnson, MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
3 |
| 15 (>13) [2] | 250.86 | ABI 3100 | CO | Nicole Hahn M U F S C | |||
|
15 |
250.88 |
ABI 310 |
CO |
Re-extracted and re-amplified |
|
|
|
| 15 [2] | 257.78 | ABI 310 | ID | Johnson_E USACIL | Re-extracted and re-amplified | Reference Blood | |
| 15 [3] |
257.74 257.78 |
ABI 3100 AVANT | ID | Julie M. Marschner, Las Vegas Metrophoitan PD Forensic Laboratory | Re-amplification of reference standard | 4 casework samples with matching reference standard of victim | 1 (5 times in one case) |
|
16 |
261.68 |
ABI 3130 |
ID/CO |
Jan Nicklas,VT Forensic Laboratory |
Re-amp |
|
|
|
16 (>13) |
300.94 |
ABI 310 |
PP |
Jackie Johnson, MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
1 in 33739 |
| 17 (>13) | 265.69 | ABI 3130xl | ID | Sherri Phillips, Washington State Patrol CODIS Crime Laboratory | Re-extracted | ||
| 18 (>13) | 269.52, 269.63 (ID); 263.09, 262.88 (CO) | ABI 3130 | CO, ID | Laboratoire de sciences judiciaires et de médecine légale, Montréal, Canada | Re-amplified, re-analyzed | 2 times in the same case |
23 reported variants as of 04/20/2016
Tri-Allelic Patterns
|
Allele Designation |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
|
6, 8, 10 |
ABI 377 |
CO |
Chris Piwonka Texas DPS
|
Re-extracted |
Convicted offender |
1 in 39,000 |
|
6, 8, 10 [2] |
ABI 3100 |
ID |
Misty Marra M U F S C |
Not verified/confirmed |
Alabama Department of Forensic Science Convicted Offender |
1 in 7840 |
|
6, 8, 10 [3] |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 6, 8, 10 [4] | ABI 3130xl | ID | Monica Price, WA Sate Patrol Crime Lab | Re-extracted | Convicted Offender | |
| 6, 8, 10 [5] | ABI 3130 | ID | Brenda Crosby, St. Louis Metropolitan Police Department Crime Lab | Re-extracted | Allele sizes: (222.11, 230.0, 237.97) [1]; (221.76, 229.66, 237.61) [2] | |
| 6, 8, 10 [6] | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-extraction | Convicted offender, Type 2 | |
|
6, 9, 10 |
ABI 377 |
CO |
Kelly Solis Texas DPS |
Re-extracted |
Convicted offender |
1 in 39,000 |
|
6, 9, 10 [2] |
ABI 377 |
In-house multiplex |
Martin Whittle, |
Re-amplification as a monoplex |
Observed during routine parentage testing, Brazilian caucasian |
1 in 25154 |
|
6, 9, 10 [3] |
ABI 3100 |
CO |
Jan Nicklas,VT Forensic Laboratory |
Re-amp |
6=9=10 |
|
|
6, 9, 10 [4] |
ABI 3100 |
ID |
Paul Mazur |
Multiple sample amplifications |
Casework samples |
|
|
6, 9, 10 [5] |
ABI 3100 |
CO |
Nicole Hahn
M U F S C |
Re-amplified x 1 |
|
1 |
| 6, 9, 10 [6] | ABI 3130XL | ID | Marie-Beatrice Tonanny & Frederic G. Brard,IRCGN,National Forensic Institute of the French Gendarmerie,Rosny-sous-Bois France | Re-extraction and Re-amplification |
FTA Card - saliva Type 2 tri-allelic pattern |
2 in 137,000 |
| 6, 9, 10 [7] | ABI 310, ABI 3500 | ID, PP | Armando Cinturão Semo, Instituto Nacional de Medicina Legal-Delegacao de Centro, Coimbra-Portugal | 2 Chelex 100 5% extraction and 2 amplification | Changana ethnic population group from Mozambique | 1 in 160 |
| 6, 9, 10 [8] | ABI 3500 | PowerPlex Fusion 6C | Erica Mpoy, St. Louis Metropolitan Police Department Crime Lab | Re-extract | Observed in a reference sample | 1 |
|
6, 10, 11 |
ABI 377 |
CO |
Chris Piwonka Texas DPS
|
Re-extracted |
Convicted offender |
1 in 39,000 |
|
6, 10, 11 [2] |
ABI 3100 |
ID |
Laura Kuyper M U F S C |
None |
|
3 in 15595 |
|
6, 10, 11 [3] |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 6, 10, 11 [4] | ABI 3100 | CO | Nicole Hoffman M U F S C | No verification - previously seen | 4 | |
| 6, 10, 11 [5] | ABI 310, ABI 3500 | ID, PP | Armando Cinturão Semo, Instituto Nacional de Medicina Legal-Delegacao de Centro, Coimbra-Portugal | 2 Chelex 100 5% extraction and 2 amplification | Macua ethnic population group from Mozambique | 1 in 160 |
|
6, 10, 12 |
ABI 310 |
CO |
Diane Hanson |
Re-extracted, observed in known and question samples |
|
|
| 6, 10, 12 [2] | ABI 3130 | ID | Jodi Luedemann, Erie County CPS Forensic Lab, Buffalo NY | Identified in both evidence and known sample (multiple sample amplifications) | Casework | |
|
7, 8, 10 |
ABI 310 |
PP & ID |
Angelo ABATI, |
2 extractions and 2 reun in PP 16 and 2 runs in ID |
|
1 in 1100 |
|
7, 8, 10 [2] |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
7, 9, 10 |
ABI 3100 |
CO |
Neil Quigley Molecular Pathology Laboratory Network, encode Paternity Systems |
Repeated DNA isolation from original buccal cells sample, and re-ran Cofiler analysis |
Routine paternity test |
l in about 20,000 |
| 7, 9, 10 [2] | ABI 3130 | ID | Rebecca Wallman, Suffolk County Crime Lab, Hauppauge NY | Re-amplification and re-run with COFiler | Reference sample | 1 |
|
7, 10, 11 |
ABI 377 |
CO |
Karl Morton Texas DPS |
Re-extraction |
offender |
1 in at least 20,000 |
|
7, 10, 11 [2] |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 7, 10, 11 [3] | ABI 310, ABI 3500 | ID, PP | Armando Cinturão Semo, Instituto Nacional de Medicina Legal-Delegacao de Centro, Coimbra-Portugal | 2 Chelex 100 5% extraction and 2 amplification | Changana ethnic population group from Mozambique | 1 in 160 |
| 8, 9, 9 | ABI 3500 | ID | Brittany Porter, Oklahoma City Police Department | Re-amplification to confirm | Two forensic unknown samples | |
|
8, 9, 10 |
ABI 377 |
CO |
Karl Morton Texas DPS |
Re-extraction |
offender |
1 in at least 20,000 |
|
8, 9, 10 [2] |
ABI 377 ABI 310 |
CO |
William Stubbs WA Sate Patrol |
Re-extracted and amplified |
Convicted offender
|
2 in 30,000 |
|
8, 9, 10 [3] |
ABI 310 |
ID |
Mark Kalinowsk |
In both questioned samples and known sample |
|
1 |
|
8, 9, 10 [4] |
FMBIO |
PP |
|
Re-extracted and re-amplified
|
Paternity samples |
1 in 1668 |
|
8, 9, 10 [5] |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Not repeated - TPOX tri-alleles very common in
|
Alabama Department or Forensic Science Convicted Offender |
4 in 11753 |
|
8, 9, 10 [6] |
ABI 377 |
In-house multiplex |
Martin Whittle, |
Re-amplification as a monoplex |
Observed during routine parentage testing, Brazilian caucasian |
1 in 25154 |
|
8, 9, 10 [7] |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
1 in 11500 |
|
8, 9, 10 [8] |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
8, 9, 10 [9] |
ABI 3100 AVANT |
ID |
Cintia Fridman, USP, |
Re-amplification |
Missing Children Data Base |
1 |
| 8, 9, 10 [10] | ABI 310 | ID | Overson_T USACIL | Re-extracted and re-amplified | Reference Blood | |
| 8, 9, 10 [11] | ABI 310 | ID | Overson_T USACIL | Re-extracted and re-amplified | Reference Blood | |
| 8, 9, 10 [12] | Manual | Promega CTT | Laboratorio de Estudios Geneticos Aplicados - Universidad Nacional de Qualimes - Argentina | Re-amplified and re-analyzed | 1 in 1325 | |
| 8, 9, 10 [13] | ABI 310 | PP |
Melissa Martinenza Deleware Office of the Chief Medical Examiner, DNA Unit |
Re-extracted and re-amplified | 2 | |
| 8, 9, 10 [14] | ABI 310 | CO | Hal Johnson, Illinois Sate Police | Samples Re-amplification | Observed in suspect standard | |
| 8, 9, 10 [15] | ABI 310 | CO | Marci Adkins, KY State Police Forensic Lab | 2 different samples, each amplified twice | A balanced triallele 8, 9, 10 @ TPOX | |
| 8, 9, 10 [16] | ABI 3730xl | PP | STAB VIDA, Portugal | Re-extraction and re-amplify | Saliva in FTA card | |
| 8, 9, 10 [17] | ABI 3730 | ID, ID Plus | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-amplified and re-analyzed with ABI 3730 | 1 in 31,330 | |
| 8, 9, 10 [18] | ABI 3130 & ABI 310 | ID | Ranee Ho, St. Louis Metropolitan Police Department Crime Lab | Tri-allelic pattern was present in individual's reference standard as well as two evidentiary samples | Allele sizes: 230.07, 233.98, 237.89 | 3 |
| 8, 9, 10 [19] | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | re-extracted, re-amplified & re-analyzed | immigration case (sibling 1; sibling 2) | 2 in 561 |
| 8, 9, 10 [20] | ABI 3130xl | ID | Cintia Fridman, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil | re-amplification | allele sizes: 230.41, 234.34, 238.36 | 2 in 2052 |
| 8, 9, 10 [21] | ABI 3130 | ID | Amy Rathmann, St. Louis Metropolitan Police Department Crime Lab | Re-extracted | ||
| 8, 9, 10 [22] | ABI 3500 | ID | Sarah Custis, St. Louis Metropolitan Police Department Crime Lab | Tri-allele present in two samples in same case | allele sizes: 228.98, 232.94, 237.03 | |
| 8, 9, 10 [23] | ABI 3130 | ID | Ashley Trausch, St. Louis Metropolitan Police Dept. Crime Laboratory | Re-extracted | Reference standard | |
| 8, 9, 10 [24] | ABI 3500xl | Applied Biosystems GlobalFiler | Ryan DeWane, South Carolina Law Enforcement Division | Re-amplification | Type 2, Balanced peak heights | 1 |
| 8, 9, 10 [25] | ABI 3500 | PowerPlex Fusion 6C | Pamela Larkin, St Louis Metropolitan Police Department Crime Lab | tri-allele was seen in the victim’s reference as well as 3 evidence samples | 4 | |
| 8, 9, 10 [26] | ABI 3500 | PowerPlex Fusion 6C | Pamela Larkin, St Louis Metropolitan Police Department Crime Lab | re-extracted | 1 | |
| 8, 9, 10 [27] | ABI 3500xl | GlobalFiler | Ryan DeWane, South Carolina Law Enforcement Division | Re-amplification | Type 2, Balanced peak heights | 3 |
| 8, 9, 11 | ABI 3100 | PR, ID | Chun Byung-Won, NISI, Seoul, Republic of Korea | Re-extraction and re-amplification | Casework sample | |
| 8, 9, 11 [2] | ABI 3100 | PP | Sun Hongyu, Chen Wenjing, Xue Tianyu. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China | Re-extraction, re-amplification with PP16, confirmation with AGCU 17+1 STR Kit (AGCU ScienTech, China) | Paternity Samples, Type 2 | 1 in 28252 |
| 8, 9, 11 [3] | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3130 | 1 in 31,330 | |
| 8, 9, 11 [4] | ABI 3130 | PP16 HS, ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-amplified and re-analyzed with ABI 3130 | 4 in 31,330 | |
| 8, 9, 11 [5] | ABI 3500 | PP, ID | Jung-Yoon Lee, NFS-East District, Won-ju, South Korea | Re-extraction and re-amplification | casework sample | |
| 8, 10, 10 | ABI 310 | Identifiler Plus | We detected a tri allele with a 10 duplication in Tpox. It was noticed in the mother (9,10) and son (8,10). The 10 was about double the height of the other allele. - Allele Size: 237.96 | |||
|
8, 10, 11 |
ABI 377 |
CO |
Chris Piwonka Texas DPS
|
Re-extraction |
Convicted offender |
1 in 39,000 |
|
8, 10, 11 [2] |
ABI 310 |
CO |
Patricia BencivengaFlorida Dept of Law Enforcement |
Re-extracted, present in both known and question samples |
Distruction/vandalisn offenses |
|
|
8, 10, 11 [3] |
ABI 310 |
PP & ID |
CintiaAlvesI PATIMUP, Porto |
Re-extracted and re-amplified |
|
1 in 145 |
|
8, 10, 11 [4] |
ABI 3100 |
ID |
Laura Kuyper M U F S C |
Not verified |
Alabama Department of Forensic Science Convicted Offender |
1 in 8400 |
|
8, 10, 11 [5] |
FMBIO |
PP |
Dayse Silva State University of
|
Re-extracted and re-amplified
|
Paternity samples |
1 in 417 |
|
8, 10, 11 [6] |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Not verified |
Alabama Department of Forensic Science Convicted Offender |
2 in 12,293 |
|
8, 10, 11 [7] |
ABI 377 |
In-house multiplex |
Martin Whittle, |
Re-amplification as a monoplex |
Observed during routine parentage testing, Brazilian caucasian Mother has genotype 9/10/11, child has 8/10/11 |
1 in 25154 |
|
8, 10, 11 [8] |
ABI 3100 |
ID |
Mark Kalinowski, |
|
Both in questioned and known samples |
1 |
|
8, 10, 11 [9] |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
8, 10, 11 [10] |
ABI 3100 |
CO |
Misty Marra M U F S C |
Re-amplification |
|
2 in 16,579 |
|
8, 10, 11 [11] |
ABI 3100 |
CO |
Angela Withrow M U F S C |
Previously documented in our lab – not repeated |
|
4 |
|
8, 10, 11 [12] |
ABI 3100 |
CO |
Angela Withrow M U F S C |
Previously documented in our lab – not repeated |
|
4 |
|
8, 10, 11 [13] |
ABI 3100 |
CO |
Angela Withrow M U F S C |
Previously documented in our lab – not repeated |
|
5 |
|
8, 10, 11 [14] |
ABI 3100 |
CO |
Nicole Hahn M U F S C |
Previously documented in our lab – not repeated |
|
6 |
|
8, 10, 11 [15] |
ABI 3100 |
CO |
Nicole Hahn M U F S C |
Previously seen, no verification |
|
8 |
|
8, 10, 11 [16] |
ABI 3100 |
CO |
Nicole Hahn M U F S C |
Previously seen, no verification |
|
8 |
|
8, 10, 11 [17] |
ABI 3100 |
CO |
Nicole Hahn M U F S C |
Previously seen, no verification |
|
10 |
|
8, 10, 11 [18] |
ABI 3100 |
CO |
Nicole Hahn M U F S C |
Previously seen, no verification |
|
11 |
| 8, 10, 11 [19] | ABI 310 | CO | Katrina Gomez, Illinois State Police | Observed in vaginal swab F1,F2 plus victim standard | ||
| 8, 10, 11 [20] | ABI 3130 | ID | Yue Wang, Center for Medical Genetics, Houston TX | Re-extracted and re-amplified | Maternity Sample | 1 |
| 8, 10, 11 [21] | ABI 310 | PP | Ashley Hayes, Anne Aruncel Co Police Crime Lab | Re-extracted and Re-amplify | 1 sample (blood from crime scene) | 1 |
| 8, 10, 11 [22] | ABI 3100 | ID | Matsuzaki, L. N.; Cantagalli, V. D.; Coudry, R. A; Fleury – Medicina e Saúde | Re-tested with PowerPlex 16 kit | Paternity samples | 1 in 7199 |
| 8, 10, 11 [23] | ABI 3130 | PP16 | Ashley Wang, Delaware OCME | Re-extracted and re-amplified | Convicted offender sample | 2 |
| 8,10,11 [24] | ABI 3130 | CTTV | Linda Bucket, Loma Linda University Medical Center | Marrow unrelated donor and recipient with 100% engraftment; re-amplification | Balanced peak heights | 2 |
| 8,10,11 [25] | ABI 3730xl | PP | STAB VIDA, Portugal | Re-extraction and re-amplify | Saliva in FTA card | |
| 8, 10, 11 [26] | ABI 310 | ID | Alejandra Torres, Laboratorio Genia, Montevideo, Uruguay | Reextracción y reamplificación | Balanced peak heights (Type 2 tri-allelic pattern) | 1 |
| 8, 10, 11 [27] | ABI 3130xl | ID Plus | Valerie Huebner, TX DPS Houston | Re-injection (allele sizes: 229.76 / 237.72 / 241.69) | 8 | |
| 8, 10, 11 [28] | ABI 310, ABI 3500 | ID, PP | Armando Cinturão Semo, Instituto Nacional de Medicina Legal-Delegacao de Centro, Coimbra-Portugal | 2 Chelex 100 5% extraction and 2 amplification | Macua ethnic population group from Mozambique | 2 in 160 |
| 8, 10, 11 [29] | ABI 3100xl | PP16 | Melissa Arria, Venezuelan Institute for Scientific Research | Re-amplification | Type 2 tri-allelic pattern. Patient with Myocardial Infarction. (STR made for genomic control in a SNP association study) | 1 in 310 |
| 8, 10, 11 [30] | ABI 3130 | ID & PP16 | Lidia Birolo (IPATIMUP) | Reamplification | Type 2; female; Brazilian Amazon individual; blood anonymous sample for Acre database | 1 in 503 |
| 8, 10, 11 [31] | ABI 3500 | PP21 | Massimo Mangiola - Rhode Island Blood Center | Re-amplified & re-analyzed | Immigration case (CH only) >70% balanced peaks | 1 in 2730 |
| 8, 10, 11 [32] | ABI 3500 | PP16 | Armando Serra, National Institute of Legal Medicine and Forensic Sciences, Coimbra-Portugal | GlobalFiler - direct amplification from a bloodstain. PP16-buccal swab (Prep-n-Go) | Child's profile in routine paternity testing with Brazilian mother and Portuguese father | |
| 8, 10, 11 [33] | ABI 3500 | PowerPlex Fusion 6C | Erica Mpoy, St. Louis Metropolitan Police Department Crime Lab | Re-extracted | Variant was seen in the victim’s reference - Allele Size: 410.65, 418.58, 422.58 | 1 |
| 8, 10, 11 [34] | ABI 3500 | PowerPlex Fusion 6C | Pamela Larkin, St Louis Metropolitan Police Department Crime Lab | tri-allele seen in reference sample and evidence samples. | 6 | |
| 8, 10, 11 [35] | ABI 3500 | GlobalFiler | Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section | re amplified | Convicted Offender - Allele Size: 350.76, 358.82, 362.83 | 1 |
|
8, 10, 12 |
ABI 377 |
CO |
Chris Piwonka Texas DPS |
Re-extracted |
Convicted offender |
1 in 39,000 |
|
8, 10, 12 [2] |
ABI 310 |
PP & ID |
CintiaAlves IPATIMUP, Porto |
Re-extracted and re-amplified |
|
1 in 145 |
|
8, 10, 12 [3] |
FMBIO |
PP |
Dayse Silva State University of
|
Re-extracted and re-amplified
|
Paternity samples |
1 in 556 |
|
8, 10, 12 [4] |
ABI 377 |
In-house multiplex |
Martin Whittle, |
Re-amplification as a monoplex |
Observed during routine parentage testing, Brazilian caucasian |
1 in 25154 |
|
8, 10, 12 [5] |
ABI 3100 |
ID |
Cantagalli,V.D.;Matsuzaki,L.N.;Coudry,R.A.; Fleury – Medicina e Saúde |
Re-tested with PowerPlex 16 kit |
Bone marrow donor |
1 in 7199 |
| 8, 10, 12 [6] | ABI 3130 | PP16 | Ashley Wang, Delaware OCME | Re-extracted and re-amplified | Convicted offender sample | 2 |
|
8, 11, 12 |
ABI 3100 |
PS & CO |
Misty Marra M U F S C |
Re-amplification |
|
1 in 10613 |
|
8, 11, 12 [2] |
Manual |
Promega Silver Staining Kit |
David Baumgarten American Red Cross |
Re-extraction and re-amplification |
|
2 in 1350 |
| 8, 11, 12 [3] | ABI 3100 | PP | ICMP | Re-extraction and amplification | Type 1 | l in 11000 |
| 8, 11, 12 [4] | LifeTech ProFlex Thermal Cycler 8-capillary 3500 Genetic Analyzer | GlobalFiler | Kimberly Ley, Wyoming State Crime Laboratory | Direct reamplification and typing of convicted offender sample. | 1 | |
| 8, 11, 14.3 | ABI 3130 and 3130xl | ID | Valerie Huebner, Texas Dept. of Public Safety | Present in multiple samples. Buccal swab and EC fraction of vaginal swab re-amplified | 3 (buccal swabs, vaginal swabs SP and EC fractions) | |
| 9, 10, 10 | ABI 310 | Identifiler Plus | We detected a tri allele with a 10 duplication in Tpox. It was noticed in the mother (9,10) and son (8,10). The 10 was about double the height of the other allele. - Allele Size: 237.96 | |||
|
9, 10, 11 |
ABI 377 |
CO |
Karl Morton Texas DPS |
Re-extraction |
offender |
1 in at least 20,000 |
|
9, 10, 11 [2] |
ABI 377 |
CO |
Present in both evidence and exemplar sample |
|
|
|
|
9, 10, 11 [3] |
ABI 310 |
CO |
|
Re-extracted |
Convicted Offender (known) |
1 |
|
9, 10, 11 [4] |
FMBIO |
PP |
Dayse Silva State University of
|
Re-extracted and re-amplified
|
Paternity samples |
1 in 834 |
|
9, 10, 11 [5] |
ABI 3100 |
|
Angela Withrow M U F S C |
None (very common in |
|
3 in 13913 |
|
9, 10, 11 [6] |
ABI 311 |
In-house multiplex |
Martin Whittle, |
Re-amplification as a monoplex |
Observed during routine parentage testing, Brazilian caucasians. Mother has genotype 9/10/11, child has 8/10/11 |
1 in 25254 |
|
9, 10, 11 [7] |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
1 in 11500 |
|
9, 10, 11 [8] |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 9, 10, 11 [9] | ABI 310 | ID | Overson_T USACIL | Re-extracted and re-amplified | Reference Blood | |
| 9, 10, 11, [10] | ABI 3100 | CO |
Nicole Hahn
M U F S C |
Seen previously in our lab - No verification | 4 | |
| 9, 10, 11, [11] | ABI 3100 | CO |
Nicole Hoffman
M U F S C |
Previously seen - No verification | 6 | |
| 9, 10, 11 [12] | ABI 3130xl | ID | Amanda Webb, SLED | Reamplification | 1 | |
| 9, 10, 11 [13] | ABI 3130 | ID | Ranee Ho, St. Louis Metropolitan Police Department Crime Lab | Tri-allelic pattern was present in both individual reference standard as well as the single source evidentiary profile | 2 | |
| 9, 10, 11 [14] | ABI 310, ABI 3500 | ID, PP | Armando Cinturão Semo, Instituto Nacional de Medicina Legal-Delegacao de Centro, Coimbra-Portugal | 2 Chelex 100 5% extraction and 2 amplification | Macua ethnic population group from Mozambique | 1 in 160 |
| 9, 10, 11 [15] | ABI 3130 | ID | Ashley Trausch, St. Louis Metropolitan Police Department Crime Lab | Re-extracted and re-amplified | Reference sample | 1 |
| 9, 10, 11 [16] | ABI 3130 | ID | Pamela Calogero, St. Louis Metropolitan Police Department Crime Lab | Re-amplified | Reference sample | 1 |
| 9, 10, 11 [17] | ABI 3500 | GlobalFiler | Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section | re amplified | Convicted Offender - Allele Size: 354.72, 358.75, 362.76 | 1 |
| 9, 10, 11 [18] | ABI 3500 | PowerPlex Fusion 6C | Amy Rathmann, St. Louis Metropolitan Police Department Crime Lab | Re-extracted | Reference standard | |
| 9, 10, 11 [19] | ABI 3500 | GlobalFiler Express | Steen Hartsen: British Columbia Institute of Technology | Re-amplified and re-run on the 3500 | Peak heights were almost identical. - Allele Size: 142.68, 150.97, 159.36 | 1 |
|
9, 10, 12 |
ABI 377 |
CO |
Shantel Kaster Texas DPS |
Re-amplified |
convicted offender |
1 in at least 20,000 |
|
9, 10, 12 [2] |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 9, 11, 12 | ABI 3100 | PP | Sun Hongyu, Chen Wenjing, Xue Tianyu. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China | Re-extraction, re-amplification with PP16, confirmation with AGCU 17+1 STR Kit (AGCU ScienTech, China). | Paternity Samples, Type 2 | 1 in 28252 |
| 10, 10, 11 | ABI 3130 | CO | Garon Foster, Bexar County Criminal Investigation Laboratory, San Antonio TX | Multiple samples including known standard | Tri-Allelic pattern, double dose of allele 10 | |
|
10, 11, 12 |
ABI 377 ABI 310 |
CO |
William Stubbs WA Sate Patrol |
Re-extracted and amplified |
Convicted offender
|
1 in 30,000 |
|
10, 11, 12 [2] |
ABI 3100 Avant |
ID |
John C. Barron, |
Re-amp and Re-run |
|
1 |
|
10, 11, 12 [3] |
ABI 310 |
PP |
Brandt Cassidy DNA Solutions |
Re-extract and Re-Amp |
Paternity testing - African-American |
1 in 1452 |
|
10, 11, 12 [4] |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
19 reported tri-allelic patterns as of 04/20/2016
* Use the following abbreviations for amplification kit names:
- CO: Applied Biosystems COfiler
- PR: Applied Biosystems Profiler
- PS: Applied Biosystems Profiler Plus
- ID: Applied Biosystems Identifiler
- PP: Promega PowerPlex 16
- CV: Promega CTTV
- CA: Promega CTTA
- MP: Promega MonoPlex
- Other: Please specify
Last Updated: 04/20/2016