D5S818 Variants
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|
Allele Designation |
Allele Size |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
| 4 | 121.71 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville | Re-extracted | ||
| 5 | 125.87 | ABI 3130 | ID | Jintana Pradutkanchana, Sukone Pradutkanchana,Suwit Ruengkittisakul, Forensic Medicine & Toxicology Unit, Prince of Songkla University, HadYai,Songkla,Thailand | Re-extraction of DNA and Re-amplification | Paternity Samples | 1 |
| 5 | 177.05/177.17 | ABI 3130 | AGCU EX22 （made by AGCU SicenTech Inc.) | Cao Li, Jin Xiaoping, Li Qianjin, Yu Jing, Meng Xingzi, Guo Xingzhong. - Judicial Identification Institute of Zhejiang Greentown Hospital | re-amplification with Microreader™ 21 ID , and sequenced | Observed during routine parentage testing(Observed in father and his son). | 2 |
|
6 |
108.82 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
|
| 6 [2] | 129.64 to 129.81 | ABI 3130 | ID | Laboratoire de sciences judiciaires et de médecine légale, Montréal, Canada | Re-amplified, re-analyzed | 3 times in the same case | |
| <7 | 129.74 | ABI 3130xl | ID | Monica Price, WA State Patrol Crime Lab, Seattle | Re-extracted | Convicted Offender | |
| <7 [2] | 129.73 | ABI 3130xl | ID | Robert Dorion, WA State Patrol Crime Lab, Seattle | Re-extracted | Convicted Offender | |
| 8.3 | 139.64 | ABI 3130xl | ID | Robert Dorion, WA State Patrol Crime Lab | Re-extraction | Convicted Offender | |
| 9.1 | 140.38 to 140.51 | ABI 3100 | PS | Laboratoire de sciences judiciaires et de médecine légale, Montréal, Canada | Re-amplified, re-analyzed | Caucasian reference sample | duplicate |
|
10.1 |
145.12 |
ABI 310 |
PS |
William Stubbs Washington State Forensic Lab Services Bureau |
Re-extracted and re-amplified
|
Convicted offender
|
2 in 20,000 |
| 10.1 [2] | 147.86 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville | Re-extracted | ||
| 10.1 [3] | 147.94 | ABI 3130 XL | ID | Monica Price, WA State Patrol Crime Lab | Re-extraction | Convicted offender | |
| 10.1 [4] | 144.79 | ABI 3130XL | PS | Peter McLaren, RCMP NCFS-Alberta | |||
| 10.1 [5] | 149.22 | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3730 | 1 in 31,330 | |
|
11.1 |
148.81 152.83 |
ABI 310 ABI 377 |
PS |
William Stubbs Washington State Forensic Lab Services Bureau |
Re-extracted and re-amplified |
Convicted offender |
1 in 30,000 |
|
11.1 [2] |
131.15 |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extracted & Re-amplified |
Convicted Offender |
1 in 69600 |
| 11.1 [3] | 152.19 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville | Re-extracted | ||
| 11.1 [4] | 152.08 - 152.17 | ABI 3130 XL | ID | Marie-Beatrice Tonanny & Frederic G. Brard, IRCGN, France | Re-extraction and re-amplification |
FTA card - saliva, POP 4 |
3 in 60.000 |
| 11.1 [5] | 152.52 | ABI 310 | ID | Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country | Re-amplified and re-analyzed | Criminal case sample, no suspect from the Forensic Genetics Department, Autonomous Police of the Basque Country | |
| 11.3 | 154.34 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville | Re-amplified | ||
| 12.1 | 156.56 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville | Re-amplified | ||
| 12.1 [2] | 156.33 156.40 | ABI 3130XL | ID | Laurent Cardin & Frederic G. Brard. IRCGN - National Forensic Institute of the French Gendarmerie, Rosny-sous-Bois, France | Re-extraction and Re-amplification | FTA card - saliva | 1 in 25000 |
| 12.1 [3] | 156.3 | ABI 3130XL | ID | Genetica, DNA Laboratories, Inc., Cincinnati OH | Inheritance (found in Father & Child) | ||
|
12.3 |
158.72 |
ABI 377 |
PS |
Catherine Allor |
Reamplified and Reanalyzed |
Paternity samples only |
1 in 16650 |
| 12.3 [2] | 158.34 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville | Re-amplified | ||
| 13.3 | 162.36 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville | Re-amplified | ||
| 15.3 | 170.09 | ABI 3130xl | ID Direct | Nelson Gaburo Junior, Luciano Cesar Scarpelli, Daniela Moratori, Pamela de Melo Machado, Carla Naufal Fontes, Renato Bueno de Moraes, Maria Cristina Sales, Tamyrez Ayrez Marques - Department of Molecular Biology – Diagnósticos da América - DASA | Re-amplified and re-analyzed | observed in paternity test | |
| >16 (18) | 179.44 & 179.46 | ABI 3130XL | ID | Regina Robinson KY State Police Forensic Lab, Frankfort KY | Re-analysis | ||
|
17 |
173.64 |
ABI 310 |
PS |
MO State Highway Patrol |
Re-extracted and re-amplified |
Convicted offender |
1 in 2575 to date |
| 17 [2] | 175.56 | ABI 3100 | ID |
Clay Davis Houston, Texas Police Dept. Crime Lab |
Re-injection | 5 | |
| 17 [3] | 153.48 | ABI 310 | PP 16 | Anthony J. Tambasco, Mansfield, Ohio Police Lab | Re-injection | Robbery Case, no suspect | 1 |
|
18 |
179.32 |
ABI 377 |
PS |
Catherine Allor |
Re-amplified and Reanalyzed |
Paternity samples only |
1 in 16650 |
| 18 [2] | 179.45 179.53 | ABI 3130 | ID | Sarah Cuddeback, Erie County CPS Forensic Lab | Multiple samples and injections | 1 | |
|
18 (>16) |
158.43 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
| >18 | 177.00 | ABI 3100 | PS | Nicole Hoffman M U F S C | Re-amplified | 1 | |
| 19 | 183.98 Avg & 161.50 Avg | ABI 310 | ID & PP | Lynette Stargardt, Independent Forensics. Hillside IL | Re-prepped & Re-amped & Re-extracted | Inheritance (Mother/Child) | |
| 19 [2] | 183.39 Avg | ABI 3100 | ID | Lynette Stargardt, Independent Forensics. Hillside IL | Re-prepped & Re-amped & Re-extracted | Inheritance (Mother/Child) | |
| 20 | 187.5 | ABI 3130XL | ID | Genetica, DNA Laboratories, Inc., Cincinnati OH | Inheritance (found in Father & Child) | ||
| 20 [2] | 165.27 | ABI 3130XL | PP 16 | Genetica, DNA Laboratories, Inc., Cincinnati OH | Inheritance (found in Father & Child) | ||
| 20 [3] | 187.67 | ABI 3130XL | ID | Genetica, DNA Laboratories, Inc., Cincinnati OH | Inheritance (child and father) | Also ran with powerplex 16 to verify locus of variant allele |
20 reported variants as of 10/21/2014
Tri-Allelic Patterns
|
Allele Designation |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
| 7, 9, 13 | ABI 3500xl | PowerPlex Fusion 6C | Robert Dorion, Washington State Patrol Crime Laboratory | re-injection | Convicted Offender | |
| 7, 10, 11 | ABI 3130xl | ID | Robert Dorion, Washington State Patrol Crime Lab, Seattle | Re-extracted and re-amplified | Convicted Offender | |
| 9,12,13 | ABI 3130xl | ID | Monica Price, Washington State Patrol Crime Lab, Seattle | Re-extraction | Convicted offender | |
|
10, 11, 12 .
|
ABI 310 |
PS |
Christine Moraczewski NE State Patrol Crime Lab |
Re-extracted and Re-amplified |
Convicted Offender |
1 |
| 10, 12, 13 | ABI 3130 ABI 310 | PS | Melissa Beal, Jennifer Lee, Denis Rodier & Kim Smith, Washington State Patrol Crime Lab | Re-extracted & Re-amplified | ||
|
10, 13, 14 |
ABI 310 |
ID |
Christine Ceniceros TX DPS, CNMC Lab El Paso |
Reinjectiion |
|
|
|
11, 12, 13 |
ABI 377 |
PS |
LabCorp |
re-amplified |
Different non-sperm samples gave 3 banded pattern along with the known sample |
|
|
11, 12, 13 [2] |
ABI 310 |
PS |
Jan Nicklas, VT Forensic Laboratory |
Re-amp |
11=12+13 |
|
|
11, 12, 13 [2] |
ABI 310 |
PP 16 & ID |
Cintia Alves, IPATIMUP, Porto, Portugal |
Re-extracted and Re-amplified |
Portuguese Caucasian Sample |
1 in 1267 |
| 11, 12, 13 [4] | ABI 3500xl | PowerPlex Fusion 6C | Matthew Fisher, Washington State Patrol Crime Lab | Re-direct amplification | Type 1 - Allele Size: 342.39, 346.44, 350.49 | |
|
11, 13, 14 |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extracted & Re-amplified |
Convicted Offender |
1 in 69600 |
| 13, 13, 14 | ABI 3130 | PS | Lisa Casey Washington State Patrol Crime Lab, Marysville | Re-extracted and Re-amplified |
8 reported tri-allelic patterns as of 10/21/2014
* Use the following abbreviations for amplification kit names:
- CO: Applied Biosystems COfiler
- PR: Applied Biosystems Profiler
- PS: Applied Biosystems Profiler Plus
- ID: Applied Biosystems Identifiler
- PP: Promega PowerPlex 16
- CV: Promega CTTV
- CA: Promega CTTA
- MP: Promega MonoPlex
- Other: Please specify