qPCR Workshop Materials
For workshop held at NFSTC on July 26-27, 2006 by Peter Vallone (NIST) and Cristiรกn Orrego (CA DOJ)
Handouts for PowerPoint presentations:
Introduction Quantitation Using PCR Instrumentation Signals and Probes Selected Forensic qPCR Assays: VT Alu SYBR Green and Quantifiler CA DOJ nuclear/mtDNA duplex and CA DOJ degradation triplex Maintenance, Validation, etc. Data Analysis and Troubleshooting qPCR Analysis Software for ABI 7000 & 7500
Introduction
Quantitation Using PCR
Instrumentation
Signals and Probes
Selected Forensic qPCR Assays:
VT Alu SYBR Green and Quantifiler CA DOJ nuclear/mtDNA duplex and CA DOJ degradation triplex
VT Alu SYBR Green and Quantifiler
CA DOJ nuclear/mtDNA duplex and CA DOJ degradation triplex
Maintenance, Validation, etc.
Data Analysis and Troubleshooting
qPCR Analysis Software for ABI 7000 & 7500