D18S51

Other Names
Chromosomal Location
GenBank Accession
D18
18q21.3

X91254; has 21 repeats
AP001534; has 18 repeats

Repeat: [GAAA] = GenBank top strand

top = 13 repeats GAAA
 

Reported Primers
Ref.
 PCR Primer Sequences
Set 1
7, 369
 5'-CAA ACC CGA CTA CCA GCA AC-3' (GAAA strand)

5'-GAG CCA TGT TCA TGC CAC TG-3' 
Set 2

ABI

AmpFlSTR® Profiler Plus™

Set 3

Promega

 GenePrint® PowerPlex™ 2.1

PCR Product Sizes of Observed Alleles
 

Allele (Repeat #)

Set 1

Set 2

Set 3
3' Flanking Sequence 
Repeat Structure
Ref.
7 262 bp 264 bp 286 bp     SGM Plus
8 266 bp 268 bp 290 bp AAAG AGAGAG [AGAA]8 716
9 270 bp 272 bp 294 bp AAAG AGAGAG [AGAA]9 716
9.2 272 bp 274 bp 296 bp     SGM Plus
10 274 bp  276 bp 298 bp AAAG AGAGAG [AGAA]10 716
10.2 276 bp 278 bp 300 bp     SGM Plus
11 278 bp 280 bp 302 bp AAAG AGAGAG [AGAA]11 716
12 282 bp 284 bp 306 bp AAAG AGAGAG [AGAA]12 716
12.2 284 bp 286 bp 308 bp     SGM Plus
13 286 bp 288 bp 310 bp AAAG AGAGAG [AGAA]13 716
13.1 287 bp 289 bp 311 bp    

variant allele
13.2 288 bp 290 bp 312 bp AAAG AG AGAGAG [AGAA]13 369
14 290 bp 292 bp 314 bp AAAG AGAGAG [AGAA]14 716
14.2 292 bp 294 bp 316 bp AAAG AG AGAGAG [AGAA]14 369
15 294 bp 296 bp 318 bp AAAG AGAGAG [AGAA]15 716
15.1 295 bp 297 bp 319 bp     SGM Plus
15.2 296 bp 298 bp 320 bp AAAG AG AGAGAG [AGAA]15 369
16 298 bp 300 bp 322 bp AAAG AGAGAG [AGAA]16 716
16.2 300 bp 302 bp 324 bp     SGM Plus
16.3 301 bp 303 bp 325 bp     SGM Plus
17 302 bp 304 bp 326 bp AAAG AGAGAG [AGAA]17 716
17.1 303 bp 305 bp 327 bp     SGM Plus
17.2 304 bp 306 bp 328 bp AAAG AGAGAG [AGAA]17 375
17.3 305 bp 307 bp 329 bp     SGM Plus
18 306 bp 308 bp 330 bp AAAG AGAGAG [AGAA]18 716
18.1 307 bp 309 bp 331 bp     SGM Plus
18.2 308 bp 310 bp 332 bp     SGM Plus
19 310 bp 312 bp 334 bp AAAG AGAGAG [AGAA]19 716
19.2 312 bp 314 bp 336 bp AAAG AGAGAG [AGAA]19 375
20 314 bp 316 bp 338 bp AAAG AGAGAG [AGAA]20 716
20.1 315 bp 317 bp 339 bp     SGM Plus
20.2 316 bp 318 bp 340 bp     SGM Plus
21 318 bp 320 bp 342 bp AAAG AGAGAG [AGAA]21 716
21.2 320 bp 322 bp 344 bp     SGM Plus
22 322 bp 324 bp 346 bp AAAG AGAGAG [AGAA]22 716
22.1 323 bp 325 bp 347 bp    

variant allele
23 326 bp 328 bp 350 bp AAAG AGAGAG [AGAA]23 716
23.1 327 bp 329 bp 351 bp     SGM Plus
24 330 bp  332 bp 354 bp AAAG AGAGAG [AGAA]24 716
25 334 bp 336 bp 358 bp AAAG AGAGAG [AGAA]25 716
26 338 bp  340 bp 362 bp AAAG AGAGAG [AGAA]26 716
27 342 bp 344 bp 366 bp AAAG AGAGAG [AGAA]27 716

Allelic Ladders: Commercially available from Applied Biosystems(ladder allele range: 9-27), Promega 

Common Multiplexes: AmpFlSTR® Profiler Plus™, GenePrint® PowerPlex™ 2.1

 

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