CD4
Repeat: [AAAAG] with some [AAAGG] = bottom strand (commonly used);
[TTTTC] = GenBank top strand
*Note*: A different number of repeats results from
using the top strand verses the bottom strand.
Reported Primers |
Ref.
|
PCR Primer Sequences |
Set 1
|
3
|
5'-TTGGAGTCGCAAGCTGAACTAGCG-3' (CTTTT strand)
5'-CCAGGAAGTTGAGGCTGCAGTGAA-3' (AAAAG strand) |
Set 2
|
27, 124
|
5'-TTGGAGTCGCAAGCTGAACTAGC-3' (CTTTT strand)
5'-GCCTGAGTGACAGAGTGAGAACC-3' (AAAAG strand) |
PCR Product Sizes of Observed Alleles
Allele
(Repeat #)
|
Set 1
|
Set 2
|
Repeat Structure
Using bottom strand
|
Repeat Structure
Using top strand
|
Ref.
|
4 |
134 bp |
86 bp |
[AAAAG]4 |
[TTTTC]5 |
27, 344 |
5 |
139 bp |
91 bp |
[AAAAG]5 |
[TTTTC]6 |
27, 344 |
6 |
144 bp |
96 bp |
[AAAAG]6 |
[TTTTC]7 |
27 |
7 |
149 bp |
101 bp |
[AAAAG]7 |
[TTTTC]8 |
27 |
8 |
154 bp |
106 bp |
[AAAAG]8 |
[TTTTC]9 |
27, 344 |
8' |
154 bp |
106 bp |
[AAAAG]5AAAGG[AAAAG]2 |
[TTTTC]3CTTTC[TTTTC]5 |
27 |
9 |
159 bp |
111 bp |
[AAAAG]6AAAGG[AAAAG]2 |
[TTTTC]3CTTTC[TTTTC]6 |
27 |
10 |
164 bp |
116 bp |
[AAAAG]7AAAGG[AAAAG]2 |
[TTTTC]3CTTTC[TTTTC]7 |
27, 344 |
11 |
169 bp |
121 bp |
[AAAAG]8AAAGG[AAAAG]2 |
[TTTTC]3CTTTC[TTTTC]8 |
27, 344 |
12 |
174 bp |
126 bp |
|
|
427 |
13 |
179 bp |
131 bp |
|
|
427 |
14 |
184 bp |
136 bp |
|
|
427 |
15 |
189 bp |
141 bp |
|
|
427 |
Allelic Ladders: Not commercially available at this time
Common Multiplexes:
Original Paper
CD4 Population Studies
CD4 Reference List
RETURN to STR Fact Sheet Page
RETURN TO HOMEPAGE