Primer sequences for PCR amplification of
loci in NIST Y STR 20plex
|
Locus
|
|
Primer Sequences (5’-to-3’) |
Primer Concentration (mM) |
Primer3 Tm (oC) |
|
DYS19 |
F |
NED-ACTACTGAGTTTCTGTTATAGTGTTTTT |
1.8 |
55.0 |
|
|
R |
GTCAATCTCTGCACCTGGAAAT |
1.8 |
60.5 |
|
DYS385 |
F |
VIC-AGCATGGGTGACAGAGCTA |
0.6 |
56.9 |
|
a/b |
R |
GCCAATTACATAGTCCTCCTTTC |
0.6 |
54.7 |
|
DYS388 |
F |
NED-GAATTCATGTGAGTTAGCCGTTTAGC |
1.8 |
63.4 |
|
|
R |
GAGGCGGAGCTTTTAGTGAG |
1.8 |
59.2 |
|
DYS389 |
F |
6FAM-CCAACTCTCATCTGTATTATCTATG |
1.3 |
54.2 |
|
I/II |
R |
GTTATCCCTGAGTAGTAGAAGAATG |
1.3 |
59.0 |
|
DYS390 |
F |
VIC-TATATTTTACACATTTTTGGGCCC |
0.2 |
57.2 |
|
|
R |
GTGACAGTAAAATGAAAACATTGC |
0.2 |
57.0 |
|
DYS391 |
F |
6FAM-TTCAATCATACACCCATATCTGTC |
0.2 |
57.9 |
|
|
R |
GATAGAGGGATAGGTAGGCAGGC |
0.2 |
60.0 |
|
DYS392 |
F |
NED-TAGAGGCAGTCATCGCAGTG |
1.8 |
60.2 |
|
|
R |
GACCTACCAATCCCATTCCTT |
1.8 |
57.3 |
|
DYS393 |
F |
VIC-GTGGTCTTCTACTTGTGTCAATAC |
0.4 |
54.7 |
|
|
R |
GAACTCAAGTCCAAAAAATGAGG |
0.4 |
57.7 |
|
DYS426 |
F |
VIC-CTCAAAGTATGAAAGCATGACCA |
0.2 |
59.3 |
|
|
R |
GGTGACAAGACGAGACTTTGTG |
0.2 |
59.8 |
|
DYS437 |
F |
6FAM-GACTATGGGCGTGAGTGCAT |
0.2 |
61.1 |
|
|
R |
GAGACCCTGTCATTCACAGATGA |
0.2 |
59.6 |
|
DYS438 |
F |
6FAM-CCAAAATTAGTGGGGAATAGTTG |
0.2 |
58.9 |
|
|
R |
GATCACCCAGGGTCTGGAGTT |
0.2 |
62.6 |
|
DYS439 |
F |
6FAM-TCGAGTTGTTATGGTTTTAGGTCT |
0.2 |
58.3 |
|
|
R |
GTGGCTTGGAATTCTTTTACCC |
0.2 |
60.3 |
|
DYS447 |
F |
PET-GGTCACAGCATGGCTTGGTT |
0.7 |
63.4 |
|
|
R |
GGGCTTGCTTTGCGTTATCTCT |
0.7 |
64.0 |
|
DYS448 |
F |
PET-TGGGAGAGGCAAGGATCCAA |
1.1 |
65.2 |
|
|
R |
GTCATATTTCTGGCCGGTCTGG |
1.1 |
64.3 |
|
460 |
F |
NED-GAGGAATCTGACACCTCTGACA |
0.7 |
59.3 |
|
(A7.1) |
R |
GTCCATATCATCTATCCTCTGCCTA |
0.7 |
59.1 |
|
H4 |
F |
NED-ATGCTGAGGAGAATTTCCAA |
0.4 |
57.3 |
|
|
R |
GCTATTCATCCATCTAATCTATCCATT |
0.4 |
56.2 |
|
YCAII |
F |
VIC-TGTCAAAATTTAACCCACAATCA |
0.9 |
59.3 |
|
a/b |
R |
GCAGTCTTTCACCATAAGGTTAGC |
0.9 |
57.2 |
Source: Butler et al. (2002) Forensic Sci.
Int., in press