Page last updated: 03/17/2023

D16S539 Variants


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Allele Designation

Allele Size 

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

4

257.74

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

6

237.30

ABI 377

CO

Nicole Swinton

Re-extracted and reamplified

 

 

6 [2]

233.11

ABI 310

PS,CO

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

6 [3]

233.44

ABI 310

CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

 

6 [4] 264.8 ABI 3130 PP 16 Jason Hodges, WV State Police Crime Lab Re-amplified Convicted Offender  

7

237.50

ABI 310

CO

Russell Vossbrink

Observed in 1 k and 3 q's

Casework

 

7 [2]

269.98

ABI 3100

PP

Cory Formea, IL State Police DNA Indexing Lab

Re-amplification

Convicted Offender Sample

1

8.3

267.94

ABI 310

ID

Angelo Abati, Institut de medecine legale de  LIEGE Belgium

3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele

 

1 in 800 convicted offenders (+ the father)

8.3 [2]

277.45

ABI 310

PP16

Angelo Abati, Institut de medecine legale de  LIEGE Belgium

3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele

 

1 in 800 convicted offenders (+ the father)

9.3

248.29

ABI 310

CO

MO State Highway Patrol

Re-extracted and re-amplified

Convicted Offender

1 in 4514 to date

9.3 [2]

271.71

ABI 310

ID

Angelo ABATI,

Belgium

3 times in ID

 

1 in 1100

9.3 [3]

281.26

ABI 310

PP 16

Angelo ABATI,

Belgium

3 times in PP16

 

1 in 1100

9.3 [4]

271.37

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

11.3

256.27

ABI 310

CO

David Young

Texas DPS

Multiple amplifications

Evidence samples

1 in 7500 to date

11.3 [2]

279.13

ABI 3100

ID

Angela Withrow

M U F S C

Re-amplified

Alabama Depart of Forensic Sciences Convicted Offender

3 in 12115

11.3 [3]

288.85

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

11.3 [4]

279.25

ABI 3100

ID

Paul Mazur

Erie County CPS Forensic Lab

Buffalo, NY

Multiple amplifications

Casework sample

 

12.1

258.39

ABI 310

CO

Nicole Swinton

Re-extracted and re-amplified

 

 

12.1 [2]

293.04

ABI 310

PP 1.2

Greg Sykes

 ATCC

Re-extracted and Re-amplified

:  From a colon carcinoma with integrated CMV-promoter-driven GADD45a

expression vector, ATCC cell line CRL-2579; name:  RKO-AS45-1

 

12.1 [3]

290.85

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

12.2

263.17

ABI 377

CO

Kelly Solis

Texas DPS

Re-amplified

Convicted offender

1 in 35000

13.1

297.18 (POP-4)

299.84 (POP-6)

ABI 310

PP

Greg Sykes

ATCC

Re-amplified three times and run using high resolution POP-6 polymer

allele discovered in a colon carcinoma cell line transfected with HPV E6 inserted in pCMV.3 (name designation: RKO-E6)

 

13.3

267.93

ABI 377

CO, PS

Nicole Swinton

Re-extracted and re-amplified

 

 

13.3 [2]

287.18

ABI 3100

ID

Angela Withrow

M U Forensic Science Center

Re-amplified

Alabama Depart of Forensic Sciences Convicted Offender

2 in 12,812

13.3 [3]

296.97

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 33739

13.3 [4]

287.65 +/-0.18

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

4 in 11500

14.3

272.02

ABI 337

CO

Nicole Swinton

Re-extracted and re-amplified

 

 

16

273.57

ABI 310

CO

Carolyn Booker

Reproduced in blood, saliva, hair, and fingernail samples

Discovered during validation testing

One individual

16 [2]

273.81±0.10

ABI 3100

CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

2 in 3750

16 (>15)

305.96

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

12 reported variants as of 02/23/07

 

Three Banded Allele Patterns

Allele Designation

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

7, 11, 12

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

9, 11, 12

ABI 3100

ID

Angela Withrow

 M U F S C

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender Sample

1 in 15144

10, 12, 13

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

11, 12, 13

ABI 3100

ID

Angela Withrow M U F S C

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender Sample

1 in 14767

11, 13, 14

ABI 3100

ID

Angela Withrow M U FS  C

Two extractions/Three amps

 

1 in 12812

11, 13, 14 [2]

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

12, 13, 14

ABI 377

CO

Karl Morton

Texas DPS

 

Re-extracted 

Convicted offender

1 in 39,000

12, 13, 14 [2]

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

6 reported three- banded patterns as of 6/02/2006

 

* Use the following abreviations for amplification kit names:

  • CO: PE Applied Biosystems COfilerTM
  • PR: PE Applied Biosystems ProfilerTM
  • PS: PE Applied Biosystems Profiler PlusTM
  • ID: PE Applied Biosystems IdentifilierTM
  • CV: Promega CTTVTM
  • CA: Promega CTTATM
  • PP: Promega PowerPlexTM
  • MP: Promega MonoPlexTM
  • Other: Please specify

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