D16S539 Variants
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Allele Designation |
Allele Size |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
4 |
257.74 |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
6 |
237.30 |
ABI 377 |
CO |
Re-extracted and reamplified |
|
|
|
6 [2] |
233.11 |
ABI 310 |
PS,CO |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 32660 |
6 [3] |
233.44 |
ABI 310 |
CO |
Jan Nicklas,VT Forensic Laboratory |
Re-amp |
|
|
6 [4] | 264.8 | ABI 3130 | PP 16 | Jason Hodges, WV State Police Crime Lab | Re-amplified | Convicted Offender | |
7 |
237.50 |
ABI 310 |
CO |
Russell Vossbrink |
Observed in 1 k and 3 q's |
Casework |
|
7 [2] |
269.98 |
ABI 3100 |
PP |
Cory Formea, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 |
8.3 |
267.94 |
ABI 310 |
ID |
Angelo Abati, Institut de medecine legale de LIEGE |
3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele |
|
1 in 800 convicted offenders (+ the father) |
8.3 [2] |
277.45 |
ABI 310 |
PP16 |
Angelo Abati, Institut de medecine legale de LIEGE |
3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele |
|
1 in 800 convicted offenders (+ the father) |
9.3 |
248.29 |
ABI 310 |
CO |
MO State Highway Patrol |
Re-extracted and re-amplified |
Convicted Offender |
1 in 4514 to date |
9.3 [2] |
271.71 |
ABI 310 |
ID |
Angelo ABATI, |
3 times in ID |
|
1 in 1100 |
9.3 [3] |
281.26 |
ABI 310 |
PP 16 |
Angelo ABATI, |
3 times in PP16 |
|
1 in 1100 |
9.3 [4] |
271.37 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police |
Re-amplified and re-analyzed |
|
1 in 11500 |
11.3 |
256.27 |
ABI 310 |
CO |
David Young |
Multiple amplifications |
Evidence samples |
1 in 7500 to date |
11.3 [2] |
279.13 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amplified |
|
3 in 12115 |
11.3 [3] |
288.85 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 32660 |
11.3 [4] |
279.25 |
ABI 3100 |
ID |
Paul Mazur |
Multiple amplifications |
Casework sample |
|
12.1 |
258.39 |
ABI 310 |
CO |
Re-extracted and re-amplified |
|
|
|
12.1 [2] |
293.04 |
ABI 310 |
PP 1.2 |
Greg Sykes ATCC |
Re-extracted and Re-amplified |
: From a colon carcinoma with integrated CMV-promoter-driven GADD45a expression vector, ATCC cell line CRL-2579; name: RKO-AS45-1 |
|
12.1 [3] |
290.85 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 32660 |
12.2 |
263.17 |
ABI 377 |
CO |
Kelly Solis |
Re-amplified |
Convicted offender |
1 in 35000 |
13.1 |
297.18 (POP-4) 299.84 (POP-6) |
ABI 310 |
PP |
Greg Sykes ATCC |
Re-amplified three times and run using high resolution POP-6 polymer |
allele discovered in a colon carcinoma cell line transfected with HPV E6 inserted in pCMV.3 (name designation: RKO-E6) |
|
13.3 |
267.93 |
ABI 377 |
CO, PS |
Re-extracted and re-amplified |
|
|
|
13.3 [2] |
287.18 |
ABI 3100 |
ID |
Angela Withrow M U Forensic |
Re-amplified |
|
2 in 12,812 |
13.3 [3] |
296.97 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 33739 |
13.3 [4] |
287.65 +/-0.18 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police |
Re-amplified and re-analyzed |
|
4 in 11500 |
14.3 |
272.02 |
ABI 337 |
CO |
Re-extracted and re-amplified |
|
|
|
16 |
273.57 |
ABI 310 |
CO |
Carolyn Booker |
Reproduced in blood, saliva, hair, and fingernail samples |
Discovered during validation testing |
One individual |
16 [2] |
273.81±0.10 |
ABI 3100 |
CO |
Jan Nicklas,VT Forensic Laboratory |
Re-amp |
|
2 in 3750 |
16 (>15) |
305.96 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 32660 |
12 reported variants as of 02/23/07
Three Banded Allele Patterns
Allele Designation |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
7, 11, 12 |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
9, 11, 12 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amp x 1 |
|
1 in 15144 |
10, 12, 13 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police |
Re-amplified and re-analyzed |
|
1 in 11500 |
11, 12, 13 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amp x 1 |
|
1 in 14767 |
11, 13, 14 |
ABI 3100 |
ID |
Angela Withrow M U FS C |
Two extractions/Three amps |
|
1 in 12812 |
11, 13, 14 [2] |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
12, 13, 14 |
ABI 377 |
CO |
Karl Morton
|
Re-extracted |
Convicted offender |
1 in 39,000 |
12, 13, 14 [2] |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
6 reported three- banded patterns as of 6/02/2006
* Use the following abreviations for amplification kit names:
- CO: PE Applied Biosystems COfilerTM
- PR: PE Applied Biosystems ProfilerTM
- PS: PE Applied Biosystems Profiler PlusTM
- ID: PE Applied Biosystems IdentifilierTM
- CV: Promega CTTVTM
- CA: Promega CTTATM
- PP: Promega PowerPlexTM
- MP: Promega MonoPlexTM
- Other: Please specify