SE33 Variants

Page last updated: 03/17/2023

SE33 Variants

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Allele Designation	Allele Size	Instrument	Amp Kit*	Contributor	Verification/Conformation Method(s)	Notes	Frequency
2	296.85	ABI 3500	GlobalFiler	Hussain M. Alsafiah, Arati Iyengar, Sibte Hadi, Waleed M. Alshlash, William H. Goodwin. Forensic and Applied Sciences/ University of Central Lancashire/ UK	Sequencing	These alleles were sequenced using BigDye Terminator v3.1 Cycle Sequencing Kit. The sequence structure is ct [cttt]3 c [CTTT]17 ct [cttt]3 ct [cttt]2 3' <60 bp del>	1
3	212 bp	ABI 3100	MP	IACOVACCI Giuseppe - RIS ROMA	Re-amplified and re-analyzed	Father and Son Samples
<4.2	306.33, 306.47	ABI 3500	GlobalFiler	Chandra Bagley, Laurissa Mitchell and Courtney Streeter, Sorenson Forensics	Confirmed in a questioned sample and a known sample		2
<4.2 [2]	306.41	ABI 3500	GlobalFiler		2 questioned samples		2
5.1	310.43, 310.35	ABI 3500	GlobalFiler	Jennifer Steele, San Bernardino County Sheriff’s Department – Scientific Investigations Division	Confirmed in a questioned sample and a known sample	peak height imbalance with sister allele, genotype 5.1, 27.2, observed at 14.5 % PHR and 22% PHR	2
5.2	278.93	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amp	Convicted offender
5.2 [2]	311.27	ABI 3500	GlobalFiler	Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section	re amplified & re extracted	Convicted Offender	1
5.3	279.92	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amp	Convicted Offender
6.2	282.43	ABI 3500xl	PowerPlex Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-direct amplification
6.2 [2]	315.42/315.39	ABI 3500	GlobalFiler	Sabrina Seehafer, DCI Crime Laboratory, Ankeny, IA		Variant Allele was observed in evidence sample.
7.1	286.88	ABI 3500	PowerPlex Fusion 6C	Erik Hall, St. Louis Metropolitan Police Department Crime Lab	Reamp		1
7.1 [2]	318.69	ABI 3500	GlobalFiler	Sarah Owen, Northeastern IL Regional Crime Laboratory	Reamplification		1
7.2	286.78, 286.84	ABI 3500	PowerPlex Fusion 6C	Erica Mpoy, St. Louis Metropolitan Police Department Crime Lab	None	Variant was seen in 2 evidence items	2
7.3	284.25	ABI 310	Power Plex ESX 17	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed
less than 8 (<8)	320.61	ABI 3500xl	GlobalFiler	Noora R. Al-Snan (PhD ongoing), Forensic Science Laboratory, Kingdom of Bahrain, part of PhD thesis submitted to Arabian Gulf University	reamplified and electrophoresed		1
8.3	328.39 - 328.42	ABI 3500XL	NGMS: Applied Biosystems	Maria Alejandra Castillo Cubillos - Laboratorio de Investigación Genética de Restos Humanos, Instituto Nacional de Medicina Legal y Ciencias Forenses	Different samples, (Mother and son)		2
10.3	299.81 and 299.82bp	ABI 3500	PowerPlex Fusion 6C	Karen Preiter, Ph.D., St. Louis Metro Police Dept. Crime Lab St. Louis, MO	reamplificaNon & reanalysis	evidence sample	1 of 262
11.2	299.76	ABI 310	Power Plex ESX 17	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed
11.2 [2]	339.56	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
11.2 [3]	339.55	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
12.1	305.7	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, Washington State Patrol Crime Laboratory	Re- direct amplification	Convicted Offender
12.2	343.64	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Convicted offender sample
12.2 [2]	343.74	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Convicted offender sample
12.2 [3]	343.67	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Convicted offender sample
12.2 [4]	343.82	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
12.2 [5]	343.86	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Criminal case sample
12.3	340.86	ABI 3500xl	GlobalFiler	Angela Richardson, MUFSC	Re-extracted (due to dropout at other loci) and amplified with Globalfiler		1
12.3 [2]	340.68	ABI 3130xl	Globalfiler	Asmer Aliyeva, Victoria Gellatly, Mehraj Abbasov, Arati Iyengar - University at Albany, SUNY, USA	Reamplified and confirmed	DOI: 10.1007/s00414-021-02610-2	1/934
12.3 [3]	307.78	ABI 3500XL	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-injection	Convicted offender
13.3	344.97, 345.04	ABI 3500	GlobalFiler	Kelly Lawrence, Northeastern Illinois Regional Crime Laboratory	present in both sample and standard; sample was re-extracted and reamplified		2
13.3 [2]	311.73	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amp	Convicted offender
13.3 [3]	344.9	ABI 3130xl	Globalfiler	Asmer Aliyeva, Victoria Gellatly, Mehraj Abbasov, Arati Iyengar - University at Albany, SUNY, USA	Reamplified and confirmed	DOI: 10.1007/s00414-021-02610-2	2/934
13.3 [4]	311.97/311.98	ABI 3500XL	PowerPlex Fusion 6C	La’Nisha Gittens, Palm Beach County Sheriff’s Office	None	Variant observed in both the evidence and reference sample	2
14.2	351.88	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
14.2 [2]	351.83	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
14.2 [3]	351.92	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Criminal case sample
14.3	348.21	ABI 3500	GlobalFiler	Hussain M. Alsafiah, Arati Iyengar, Sibte Hadi, Waleed M. Alshlash, William H. Goodwin. Forensic and Applied Sciences/ University of Central Lancashire/ UK	Sequencing	These alleles were sequenced using BigDye Terminator v3.1 Cycle Sequencing Kit. The sequence structure is ct [cttt]3 c [CTTT]3 C [CTTT]12 -- [cttt]3 ct [cttt]2	1
15.1	317.81	ABI 3500xl	PowerPlex Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-injection
15.2	355.90	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
15.2 [2]	352.11	ABI 3500xl	GlobalFiler	Monika Chakravarty – Forensic Science Laboratory, New Delhi India	Reisolated and reamplified		0.0016
15.3	356.88 (NGM SElect), 247.02 (SEfiler)	ABI 3130	NGM SElect; SEfiler	Magdalena Bogus, Institute of Legal Medicine, University Hospital of Cologne	Reamplified and reanalyzed	Clinical sample	1
15.3 [2]	357.12	ABI 310	NGM SElect	Ioulia Skitsa, Alexandra Ampati, DNA Analysis Laboratory, Athens Legal Medicine Department, Greece	Reference sample and victim vaginal sample (2 different extractions)	convicted offender from Pakistan	1
16.1	322.24, 322.28	ABI 3500xl	PowerPlex Fusion 6C	Sarah Ellis, North Carolina State Crime Laboratory	Re-amplified and re-analyzed	elimination standard	1
16.1 [2]	355.25	ABI 3500	GlobalFiler	Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section	re amplified	Convicted Offender	1
16.1 [3]	329.94	ABI 3500	QIAGEN Investigator 24plex QS kit	Andrew Bishop, Montana State Crime Lab	Re-amplification	Evidence sample	2
16.2	360.25	ABI 310	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Population study sample
16.2 [2]	360.00	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
16.3	320.82	ABI 310	Power Plex ESX 17	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed
16.3 [2]	357.16	ABI 3500xl	GlobalFiler	Monika Chakravarty – Forensic Science Laboratory, New Delhi India	Reisolated and reamplified		0.0016
17.1	364.44	ABI 3130xl	ESI 17	Car Di Maggio RIS di ROMA	Re-amplified and run with NGM SElect		1
17.1 [2]	325.86	ABI 3500xl	PowerPlex Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-direct amplification
less than 17.2 (around 17.1)	359.2	ABI 3500xl	GlobalFiler	Noora R. Al-Snan (PhD ongoing), Forensic Science Laboratory, Kingdom of Bahrain, part of PhD thesis submitted to Arabian Gulf University	reamplified and electrophoresed		1
17.2	363.95	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Convicted offender sample
17.2 [2]	363.95	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
17.3	364.98	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Criminal case sample, no suspect
17.3 [2]	364.97	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
17.3 [3]	364.95	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
17.3 [4]	364.99 & 364.97	ABI 3130	NGM SElect	Juan-Angel Roman, Scientific Police Division, Police of the Government of Catalonia-Mossos d'Esquadra, Spain	Re-amplified and re-analyzed		1
17.3 [5]	328.18	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, Washington State Patrol Crime Laboratory	Re- direct amplification	Convicted Offender
17.3 [6]	361.37	ABI 3500	Applied Biosystems GlobalFiler	WV State Police Forensic Laboratory Biochemistry Section	re-amplified and re-analyzed		2
17.3 [7]	328.51/328.67	ABI 3500	PowerPlex Fusion 6C	Krista Spear, St. Louis Metropolitan Police Department Crime Laboratory	Present in two questioned samples		2
17.3 [8]	328.39	ABI 3500	PowerPlex Fusion 6C	Pamela Larkin, St Louis Metropolitan Police Department Crime Lab	re-amplified		1
17.3 [9]	328.35	ABI 3500	PowerPlex Fusion 6C	Erik Hall, St. Louis Metropolitan Police Department Crime Lab	Various samples + reamp		1
17.3 [10]	328.38, 328.44, 328.45, 328.47, 328.53	ABI 3500	PowerPlex Fusion 6C	Pamela Larkin, St Louis Metropolitan Police Department Crime Lab	re-amplified	same profile in 10 evidence samples	10
17.3 [11]	328.44	ABI 3500	PowerPlex Fusion 6C	Pamela Larkin, St Louis Metropolitan Police Department Crime Lab	re-amplified		1
17.3 [12]	328.37	ABI 3500xl	PowerPlex Fusion 6C	Kathryn Hoodenpyle, St. Louis Metropolitan Police Department Crime Laboratory	Re-amplification		1 sample
17.3 [13]	361.19	ABI 3130xl	Globalfiler	Asmer Aliyeva, Victoria Gellatly, Mehraj Abbasov, Arati Iyengar - University at Albany, SUNY, USA	Reamplified and confirmed	DOI: 10.1007/s00414-021-02610-2	3/934
18.1	366.99	ABI 3130	NGM SElect	Juan-Angel Roman, Central Area of Criminalistics, Police of the Government of Catalonia-Mossos d'Esquadra, Spain	Re-amplified and re-analyzed	Criminal case sample, no suspect	9 but it is the same profile in different case samples
18.1 [2]	368.79 (ESI 17); 363.21 (Globalfiler)	ABI 3130xl	PowerPlex ESI 17 & Globalfiler Express	Paulo Dario, Carina Almeida, Ana Rita Oliveira and Teresa Ribeiro, National Institute of Legal Medicine and Forensic Sciences - South Branch, Portugal	Kit overlap, two different samples collected (blood and saliva) and analyzed with the different kits	Individual from the South of Portugal	1
18.1 [3]	330.76	ABI 3500xl	PowerPlex Fusion 6C	Matthew Fisher, Washington State Patrol Crime Laboratory	Re-amplified and re-analyzed	Convicted Offender	1
18.1 [4]	330.4	ABI 3500	PowerPlex Fusion 6C	Erica Mpoy, St. Louis Metropolitan Police Department Crime Lab	Re-amplified	Evidence sample	1
18.3	368.93	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
18.3 [2]	368.95	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
18.3 [3]	368.93	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
18.3 [4]	368.99	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
18.3 [5]	332.31	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, Washington State Patrol Crime Laboratory	Re- direct amplification	Convicted Offender
18.3 [6]	332.61, 332.5	ABI 3500	PowerPlex Fusion 6C	Erik Hall, St. Louis Metropolitan Police Department Crime Lab	Reamp
18.3 [7]	365.42	ABI 3500xl	Applied Biosystems GlobalFiler	WV State Police Forensic Laboratory Biochemistry Section	re-amplified/re-analyzed
18.3 [8]	365.46	ABI 3500xl	Applied Biosystems GlobalFiler	WV State Police Forensic Laboratory Biochemistry Section	re-amplified/re-analyzed	Variant also submitted from our lab by Joshua T Haynes 2/28/2018	3 times (Q sample, and K x2)
18.3 [9]	332.46, 332.53, 332.59, 332.52, and 332.49	ABI 3500	PowerPlex Fusion 6C	Ashley O’Leary, Saint Louis Metropolitan Police Department Laboratory	Variant allele was detected in several specimens from the same case.
18.3 [10]	332.21, 332.45 (4 times), 332.42, and 332.35	ABI 3500	PowerPlex Fusion 6C	Erik Hall, St. Louis Metropolitan Police Department Crime Lab	Numerous samples in case, both evidence samples and a reference standard
18.3 [11]	365.27	ABI 3130xl	Globalfiler	Asmer Aliyeva, Victoria Gellatly, Mehraj Abbasov, Arati Iyengar - University at Albany, SUNY, USA	Reamplified and confirmed	DOI: 10.1007/s00414-021-02610-2	1/934
19.1	334.22	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, Washington State Patrol Crime Laboratory	Re- direct amplification	Convicted Offender
19.1 [2]	367.39, 367.41, 367.45	ABI 3500	Applied Biosystems GlobalFiler	Tara Scott, Iowa DCI Crime Lab		Multiple samples in same case	3
19.1 [3]	367.44bp, 367.42bp, 367.49bp, and 367.37bp	ABI 3500	Applied Biosystems GlobalFiler	Barbara Wells, Oklahoma State Bureau of Investigation	since the same micovariant was observed in three different questioned samples, no re-amplification was performed. Subsequent submission of a suspect known and analysis of the known showed the same microvariant in the known sample as well.		four separate samples pertaining to the same case.
19.1 [4]	253.09	ABI PRISM 3130	PowerPlex ES Monoplex System	Munir Ahmad Bhinder. Dept. of Human Genetics & Molecular Biology, University of Health Sciences Lahore.	Re-amplified and re-analyzed	Population study sample	1 of 408
19.2	371.96	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
19.2 [2]	372.03	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
19.2 [3]	372.01	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample; genotype: 19.2, 22
19.2 [4]	372.09	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
19.3	372.96	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
19.3 [2]	332	ABI 3500XL	Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-injection
19.3 [3]	369.9	ABI 3500xl	GlobalFiler	Monika Chakravarty – Forensic Science Laboratory, New Delhi India	Reisolated and reamplified		0.00083
20	370.45	ABI 3500	Applied Biosystems GlobalFiler	Hortensia Cano y Valeria Marcucci. Laboratorio Regional de Investigación Forense-Santa Cruz- Argentina	Re-amplification and re-analyzed	reference samples	1
20.1	371.44	ABI 3500	Applied Biosystems GlobalFiler	Sabrina Seehafer, DCI Crime Laboratory, Ankeny, IA	Re-amplified in GlobalFiler	swabs from straw	1
20.1 [2]	257.04	ABI PRISM 3130	PowerPlex ES Monoplex System	Munir Ahmad Bhinder. Dept. of Human Genetics & Molecular Biology, University of Health Sciences Lahore.	Re-amplified and re-analyzed	Population study sample	1 of 408
20.1 [3]	338.53	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-injection	Convicted offender
20.3	273.37/273.40	ABI 3500	Applied Biosystems GlobalFiler	Amanda Misencik, DCI Crime Laboratory, Ankeny, IA	Re-amplified	Present in known & question sample, both were re-amplified	2
20.3 [2]	340.41	ABI 3500xl	PowerPlex Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-injection	convicted offender
20.3 [3]	372.46	ABI 3500	GlobalFiler	Hussain M. Alsafiah, Arati Iyengar, Sibte Hadi, Waleed M. Alshlash, William H. Goodwin. Forensic and Applied Sciences/ University of Central Lancashire/ UK	Sequencing	These alleles were sequenced using BigDye Terminator v3.1 Cycle Sequencing Kit. The sequence structure is ct [cttt]2 cctt c [CTTT]9 TTT [CTTT]11 ct [cttt]3 ct [cttt]2	1
21.1	314.3, 314.4, 314.5, 314.5	ABI 3100	iPlex STR	Liz Kopitke, Independent Forensics	Shared between mother and three children	The family in which the allele was observed is from Haiti
21.1 [2]	357.4	ABI 3500xl	GlobalFiler	Monika Chakravarty – Forensic Science Laboratory, New Delhi India	Reisolated and reamplified		0.0025
22	382.04	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Convicted offender sample
22 [2]	382.06	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Convicted offender sample
22 [3]	382.01	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [4]	382.09	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [5]	382.12	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [6]	381.99	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [7]	381.97	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [8]	382.00	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [9]	382.01	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [10]	382.08	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [11]	382.00	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [12]	382.12	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
22 [13]	382.04	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [14]	382.05	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [15]	382.05	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [16]	382.04	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [17]	382.05	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [18]	381.99	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [19]	382.04	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample; genotype: 19.2, 22
22 [20]	381.92	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
22 [21]	382.06	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
22 [22]	382.17	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
22 [23]	382.08	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
22 [24]	382.17	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
22 [25]	382.14	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Criminal case sample
22 [25]	382.11	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Criminal case sample
22 [26]	378.25	ABI 3500xl	GlobalFiler	Monika Chakravarty – Forensic Science Laboratory, New Delhi India	Reisolated and reamplified		0.0041
22.1	346.93	ABI 3500xl	PowerPlex Fusion 6C	Brendan Shanahan, Washington State Patrol Crime Laboratory	Re- direct amplification	Convicted Offender
22.1 [2]	265.02	ABI PRISM 3130	PowerPlex ES Monoplex System	Munir Ahmad Bhinder. Dept. of Human Genetics & Molecular Biology, University of Health Sciences Lahore.	Re-amplified and re-analyzed	Population study sample	1 of 408
22.1 [3]	346.8	ABI 3500xl	PowerPlex Fusion 6C	Dr Anand Kumar, Dr Ginni Kumawat, Dheeraj Singh Tanwar- State Forensic Science Laboratory, Rajasthan, India.	Re-amplification and Genotyping, Confirmed by Applied Biosystems GlobalFiler.	Reference Sample.	3
22.3	348.79	ABI 3500	PowerPlex Fusion 6C	Heather Pyles, Washington State Patrol Vancouver Crime Laboratory	reinjection	variant allele observed in evidence sample that was consumed during amplification, reamplification for verification not possible	1
23	386.14	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
23 [2]	386.05	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
23 [3]	386.11	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Criminal case sample, no suspect
23 [4]	386.05	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
23 [5]	386.10	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
23 [6]	386.25	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
23 [7]	346.79 (346.96)	ABI 3130	PowerPlex ESX 17	Victor Inglés, Central Area of Criminalistics, Police of the Government of Catalonia-Mossos d'Esquadra, Spain	Re-amplified and re-analyzed	Son	1
23 [8]	346.82 (346.94)	ABI 3130	PowerPlex ESX 17	Victor Inglés, Central Area of Criminalistics, Police of the Government of Catalonia-Mossos d'Esquadra, Spain	Re-amplified and re-analyzed	Father	1
23 [9]	382.6	ABI 3500xl	GlobalFiler	Monika Chakravarty – Forensic Science Laboratory, New Delhi India	Reisolated and reamplified		0.00083
23.1	350.59	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amp	Convicted offender
23.1 [2]	383.37	ABI 3500	GlobalFiler	Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section	re amplified	Convicted Offender	1
23.3	353.06	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amp	Convicted Offender
23.3 [2]	385.48/385.52/385.51	ABI 3500	Globalfiler	Sabrina Seehafer, DCI Crime Lab	Seen in multiple samples	Epithelial fraction & known buccal	2
24	390.21	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
24 [2]	390.16	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
24.1	387.57, 387.43	ABI 3500	GlobalFiler	Tina Gore, Scottsdale Police Department Crime Lab, DNA Unit	Multiple samples tested	From the sperm fraction of external genital and vaginal swabs
24.1 [2]	355.15	ABI 3500xl	PowerPlex Fusion 6C	Brendan Shanahan, Washington State Patrol Crime Laboratory	Re- direct amplification	Reference Sample
24.3	389.58	ABI 3500	GlobalFiler	Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section	re amplified	Convicted Offender	1
24.3 [2]	356.76 bp	ABI 3500XL	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-injection	Convicted offender
25	390.49 (390.62)	ABI 3500	Applied Biosystems GlobalFiler	Hortensia Cano y Valeria Marcucci, Laboratorio Regional de Investigacion Forense. Santa Cruz-Argentina	Re-amplified and re-analyzed	reference sample and forensic sample	1
25.1	358.75	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amp	Convicted offender
25.3	360.92	ABI 3500XL	Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-injection
25.3 [2]	360.59 bp	ABI 3500XL	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-injection	Convicted offender
26	394.68	ABI 3500	Applied Biosystems GlobalFiler	Hortensia Cano y Valeria Marcucci. Laboratorio Regional de Investigación Forense-Santa Cruz- Argentina	Re-amplification and re-analyzed	reference samples	1
26.3	397.65/397.68	ABI 3500	Applied Biosystems GlobalFiler	Amanda Misencik, Iowa DCI Criminalistics Laboratory	Re-amplified	Reference sample	1
26.3 [2]	364.8	ABI 3500	PowerPlex Fusion 6C	Krista Spear, St. Louis Metropolitan Police Department Crime Lab	Multiple samples	Apparent in multiple samples from the same case	1
27.3	369.28	ABI 3500xl	PowerPlex Fusion 6C	Matthew Fisher, Washington State Patrol Crime Laboratory	Re-amplified and re-analyzed	Convicted Offender	1
27.3 [2]	401.48	ABI 3130xl	Globalfiler	Asmer Aliyeva, Victoria Gellatly, Mehraj Abbasov, Arati Iyengar - University at Albany, SUNY, USA	Reamplified	DOI: 10.1007/s00414-021-02610-2	1/934
28	406.21	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
28.1	370.50; 371.30	ABI 3500	PowerPlex Fusion 6C	Ashley O’Leary; Saint Louis Metropolitan Police Department Crime Laboratory	Re-amplification
28.3	372.74	ABI 3500	PowerPlex Fusion 6C	Sean Carhart, Washington State Patrol	Re-extracted, amplified, and analyzed the questioned sample. Also present in reference sample.		1
28.3 [2]	372.83	ABI 3500	PowerPlex Fusion 6C	Kathryn Hoodenpyle, St. Louis Metropolitan Police Department Crime Laboratory	Reamplified		1
28.3 [3]	405.64	ABI 3500	Globalfiler	Ian Perry; West Virginia State Police Forensic Lab, Biology Unit: Databasing Section	re-extraction and reamplification	Offender Sample	1
29	410.08	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
29 [2]	410.21	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
29 [3]	410.15	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
29 [4]	410.18	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
29 [5]	410.16	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
29 [6]	410.05	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
29.3	409.40, 409.54, 409.46	ABI 3500	GlobalFiler	Kelly Lawrence, Northeastern Illinois Regional Crime Laboratory	re-injected and re-amplified Allele Size: 409.40 (first amp), 409.54 (rerun first amp), and 409.46 (second amp)	suspect standard	1
29.3 [2]	376.91	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-injection	Convicted offender
30	414.13	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
30.1	411.59	ABI 3500	GlobalFiler	Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section	re amplified	Convicted Offender	2
30.3	381.11	ABI 3500XL	Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-injection
31	418.16	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
31.3	385.2, 385.18, 384.94	ABI 3500xl	PowerPlex Fusion 6C		Confirmed in three intimate sources belonging to the reference		1
32.1	419.63 (x3)	ABI 3500	Applied Biosystems GlobalFiler	Joe Orcutt, Oklahoma State Bureau of Investigation	Present in two questioned samples and one known sample	One of the questioned samples in which the variant allele is observed is an intimate sample (penile swab) collected from the same individual for which the variant allele is present in the known reference profile (profiles match).	3
33	426.37	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
33 [2]	426.38	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Criminal case sample, no suspect
33 [3]	426.26	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Criminal case sample, no suspect
33 [4]	426.29	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
33 [5]	426.31	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
33 [6]	426.44	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Criminal case sample, no suspect
33 [7]	426.34	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and reanalyzed	Convicted offender sample
33 [8]	426.28	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
33 [9]	426.26	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
33 [10]	426.31	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
33 [10]	426.39	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
33 [11]	426.34	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
33 [12]	426.31	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
33 [13]	426.40	ABI 3500	NGM SElect	Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample
34	430.40	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Convicted offender sample
34 [2]	430.33	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Re-amplified and re-analyzed	Convicted offender sample
35	371.48 371.38 371.64 371.53	ABI 3130xl	COrDIS	Igor Kornienko, The Southern Scientific Centre of the Russian Academy of the Sciences	reamplification	forensic case, Armenian nationality	1
36	434.73 (GlobalFiler), 398.32 (ESX17)	ABI 3500	GlobalFiler and PowerPlex ESX 17	Anne De Bast, DNA Identification BIO.be - Gosselies, Belgium	Re-extraction and amplification with other kit	reference sample and forensic sample	1 case
38	441.70	ABI 3500	GlobalFiler	Hussain M. Alsafiah, Arati Iyengar, Sibte Hadi, Waleed M. Alshlash, William H. Goodwin. Forensic and Applied Sciences/ University of Central Lancashire/ UK	Sequencing	These alleles were sequenced using BigDye Terminator v3.1 Cycle Sequencing Kit. The sequence structure is ct [cttt]2 cctt c [CTTT]9 CTTTTT [CTTT]12 CTTTTT [CTTT]14 ct [cttt]3 ct [cttt]2	1
41	422.15	3500xL Genetic Analyzer	Promega PowerPlex Fusion 6C	Jeannie Berg – North Carolina State Crime Laboratory	re-amplification		four times observed

31 reported variants as of 11/15/17

Three Banded Allele Patterns

Allele Designation	Instrument	Amp Kit*	Contributor	Verification/Conformation Method(s)	Notes	Frequency
<4.2, 19, 26.2	ABI 3500	GlobalFiler	Chandra Bagley, Laurissa Mitchell and Courtney Streeter, Sorenson Forensics	Confirmed in a questioned sample and a known sample		2
5.1, 14, 27.2	ABI 3500	GlobalFiler	Crystal Beepot - Institute of Forensic Science and Legal Medicine	Observed in two samples		2
12, 14, 18	ABI 3500	GlobalFiler	Laboratorio de Genética Forense de Ministerio Público Fiscal de Tucumán, Argentina	Re-extraction and Re-amplified	Type 1 - Allele Size: 338pb (5349), 346pb (3218), 362pb (7863)	1
13, 15, 17	ABI 3500XL	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amplification	Convicted Offender, Type 1
13, 19, 20	ABI 3500XL	PowerPlex Fusion 6C	Timothy Baize, North Carolina State Crime Lab	Reamplified and reanalyzed	Reference sample, Type I tri-allele (13 ~ 19+20)
14, 18, 19	ABI 3500XL	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-amplification	Convicted offender, Type 1
14, 21, 22	ABI 3500xl	PowerPlex Fusion 6C	Sarah Ellis, North Carolina State Crime Laboratory	Re-amplified and re-analyzed	type 1 pattern, reference standard (14 ~ 21 + 22) - Allele Size: 14 (2839), 21(1584), 22 (1163)
14, 29.2, 30.2	ABI 3500xl	PowerPlex Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-direct amplification	convicted offender - Allele Size: 313.11, 376.18, 380.30
14.2, 29.2, 30.2	ABI 3500	GlobalFiler	Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section	re amplified - Allele Size: 348.15, 408.54, 412.56	Convicted Offender	1
15, 19, 20	ABI 3500	GlobalFiler	Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section	re amplified - Allele Size: 350.07, 366.35, 370.4	Convicted Offender	1
15, 20, 21	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-extraction	Convicted offender, Type 1
16, 18, 19	ABI 3500XL	Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-direct amplification	Type 1 - Allele Size: 320.63, 328.84, 332.97
16, 19, 20	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Convicted offender sample, type 2 tri-allele? (19 looks like high stutter, 16 and 20 fairly balanced)
17, 18, 19	ABI 3500	PowerPlex Fusion 6C	Gloria Vallejo, Begoña Santiago, Belén Alcalá, Pilar Martínez, Biology Service. National Institute of Toxicology and Forensic Sciences. Department of Madrid. Spain	Amplified in two different kits	paternity study sample, type 1 tri-allelic pater (Fusion 6C: 325.11, 329.22, 333.33.; GF: 358.30, 362.42, 366.43))	1
17, 18, 19.2	ABI 3500xl	PowerPlex Fusion 6C	Samantha Chit Khin, North Carolina State Crime Lab	reamplified & reanalyzed	Allele Size: first amp: 1186, 2482, 3555; second amp: 1460, 3703, 4485 - Criminal case sample, Type 1 tri-allele (17+18~19.2)
17, 18, 30.2	ABI 3500xl	PowerPlex Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-direct amplification	convicted offender - Allele Size: 325.28, 329.30, 380.23
17, 25.5, 27.2	ABI 3500XL	Fusion 6C	Matthew Fisher, Washington State Patrol Crime Lab	Re-direct amplification	Type 1 - Allele Size: 324.71, 359.51, 367.59
18, 19, 21	ABI 3500	GlobalFiler		confirmed with re-amplification	Allele Size: 362.36, 366.31, 374.33
18, 19, 23	ABI 3500XL	GlobalFiler and PowerPlex Fusion 6C	Marcel Burton, Palm Beach County Sheriff’s Office, Forensic Biology Unit	ran on rapidHit with Globalfiler kit	Elimination sample Allele Size: 18 (329.18), 19 (333.26), 23 (349.65)
18, 21, 22	ABI 3500	Promega PP Fusion 6 and Promega GlobalFiler	Martínez P, Alcalá B, Santiago B, Vallejo G. National Institute of Toxicology & Forensic Science. Biology Service. José Echegaray 4, 28232 Madrid, Spain	Promega GlobalFiler kit STRs edition	TYPE I, Allele Size: 329.20, 341.53, 345.65	5 PCRs from two samples
18, 28.2, 28.2	ABI 3500	GlobalFiler	Eric Parry, Iowa DCI Crime Laboratory	Seen in known buccal sample and multiple question samples In the same case that were single source and matched the known.	Allele Size: #1= 18 (362.35)(661rfu), 28.2(404.71)(2295rfu) #2= 18 (362.40)(382rfu), 28.2(404.59)(2013rfu) #3= 18 (362.50)(602rfu), 28.2(404.65)(3314rfu) #4= 18 (362.39)(801rfu), 28.2(404.67)(3342rfu) #5= 18 (362.40)(556rfu), 28.2(404.67)(4674rfu) #6= 18 (362.39)(382rfu), 28.2(404.65)(2451rfu) #7= 18 (362.41)(980rfu), 28.2(404.71)(4507rfu)
18, 30.2, 31.2	ABI 3500	PowerPlex Fusion 6C	Kate Zopolos, Washington State Patrol Crime Laboratory	Re-injection	Allele Size: 329.57, 380.38, 384.50	1
18, 31.2, 32.2	ABI 3500	NGM SElect	Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country	Reamplified and reanalyzed	Criminal case sample, type 1 tri-allele (18 ~ 31.2+32.2)
19, 20, 22.2	ABI 3500	Applied Biosystems GlobalFiler	Kelly Lawrence, Northeastern Illinois Regional Crime Laboratory	sample re-amplified/re-analyzed		1
19, 20, 31.2	ABI 3500XL	PowerPlex Fusion 6C	Kelly Behnke, Palm Beach County Sheriff’s Office, Forensic Biology Unit		Type 1 tri-allele - Allele Size: 1477 rfu, 532 rfu, 2109 rfu	1
19, 30.2, 31.2	ABI 3500	GlobalFiler	Sabrina Seehafer, DCI Crime Laboratory, Ankeny, IA		Tri-allelic pattern observed in two samples in Sexual Assault kit (known & forensic) - Allele Size: 19 (366.30), 30.2 (412.47-412.55), 31.2 (416.52-416.56)
19.2, 21, 22	ABI 3500	PowerPlex Fusion 6C	Benjamin Cooley; Allegheny County Office of the Medical Examiner	Manual Reamplification	Type 1 Pattern - Allele Sizes: 335.83, 341.96, and 346.02	Once
20, 21, 23.2	ABI 3500	Applied Biosystems GlobalFiler	Sabrina Seehafer, DCI Crime Laboratory, Ankeny, IA	Re-amplified in GlobalFiler, present in single amp with Fusion 6C and Investigator 24Plex	Allele Size: 370.35, 374.40, 384.42 - employee sample	1
20, 21, 24.2	ABI 3500	PowerPlex Fusion 6C	Krysta Snyder, Oklahoma City Police Department Laboratory	present in questioned sample and a reference sample, reference reamplified and reanalyzed	Type 1 pattern
20, 21, 26.3	ABI 3500	GlobalFiler, PowerPlex Fusion 6C	Fabiano Gentile, Adriano Passero, Alberto Marino - Reparto Carabinieri Investigazioni Scientifiche di Parma - Sezione Biologia	Re-amplified and re-analyzed; confirmed in a questioned sample and a known sample	Biallelic pattern in DYS391 in the same genotype
20, 25.2, 26.2	ABI 3500XL	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-amplification	Convicted offender, Type 1
21, 22, 29.2	ABI 3500	GlobalFiler	Stanton Freilinger – Iowa DCI Criminalistics Laboratory	Re-Amplification	Reference Sample	1
21, 29.2, 30.2	ABI 3500	GlobalFiler and PowerPlex Fusion 6C	Michelle Hassler, San Diego Sheriff Crime Lab	Amplified in two different kits	Type 1 tri-allelic pattern (GF: 374.34, 408.50, 412.55; Fusion 6C: 341.79, 376.07, 380.18)
22.2, 23.2, 32.2	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amplification	Convicted offender, Type 1
23, 24, 26.2	ABI PRISM 3130XL	Applied Biosystems GlobalFiler	INPS - Laboratoire de Police Scientifique - Marseille	4 distinct samples in the same case	Allele Size: 23 : 382,29 / 24 : 386,35 / 26.2 : 396,47	1st time
23.2, 26.2, 27.2	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-extraction	Type 1, Convicted offender
23.2, 26.2, 28.2	ABI 3500	PowerPlex Fusion 6C	Benjamin Cooley; Allegheny County Office of the Medical Examiner	Manual Reamplification	Type 1 Pattern - Allele Sizes: 352.25, 364.23, and 372.33	Once
23.2, 30.2, 31.2	ABI 3500	Globalfiler	Ian Perry; West Virginia State Police Forensic Lab, Biology Unit: Databasing Section	re-extraction and reamplification	Offender Sample - Allele Size: 384.48, 412.59, 416.64	1
24.2, 26.2, 30.2	ABI 3500xl	PowerPlex Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amp	Type 1, Convicted offender
26.2, 27.2, 28.2	ABI 3500XL	PowerPlex Fusion 6C	Abby Hernandez, Washington State Patrol Crime Lab	Re-extraction	Type 1 - Allele Size: 363.85, 367.88, 371.82	1
26.2, 27.2, 31.2	ABI 3500	GlobalFiler	Tara Scott, Iowa DCI Crime Lab	Re-amplified	Allele Size: 396.69/400.68/416.68	Once
26.2, 28.2, 29.2	ABI 3500	GlobalFiler	Ian Perry; West Virginia State Police Forensic Lab, Biology Unit: Databasing Section	re-extraction and reamplification	Offender Sample (blood on FTA) - Allele Size: 396.64, 404.59, 408.62	1
26.2, 29.2, 30.2	ABI 3500	PowerPlex Fusion 6C	Amy Rathmann, St. Louis Metropolitan Police Department Crime Lab	Re-extracted	Reference standard
26.2, 30.2, 31.2	ABI 3500xl	Fusion 6C	Monica Price, WA State Patrol Crime Lab	Re-direct amp	Convicted offender, Type 1
27.2, 29.2, 30.2	ABI 3500xl	PowerPlex Fusion 6C	Matthew Fisher, Washington State Patrol Crime Laboratory	Re-extract and Re-amplify	Type 2, Allele Size: 368.17, 376.26, 380.42	1
27.2, 31.2, 32.2	ABI 3500	GlobalFiler	Sabrina Seehafer, DCI Crime Laboratory, Ankeny, IA		Tri-allelic pattern observed in multiple samples in Sexual Assault kit (4 samples) - Allele Size: 27.2 (400.60-400.70); 31.2 (416.56-416.63); 32.2 (420.62-420.73)
28.2,29.2,30.2	ABI 3500	GlobalFiler	Brent Myers, West Virginia State Police Forensic Laboratory	reamplified	Tri-allele pattern type 1	1
28.2, 30.2, 31.2	ABI 3500xl	NGM SElect, ESX17, ESSplex-SE-Plus	Car. Dario Di Maggio, RIS Carabinieri ROMA	Re amplification with three different kits	Suspect - Caucasian donor	one
29.2, 30.2, 34.2	ABI 3500	PowerPlex Fusion 6C	Rebekah Neyhart, Washington State Patrol Crime Lab	re-extracted	reference sample	1

11 reported as of 11/28/17

* Use the following abbreviations for amplification kit names:

CO: Applied Biosystems COfiler
PR: Applied Biosystems Profiler
PS: Applied Biosystems Profiler Plus
ID: Applied Biosystems Identifiler
PP: Promega PowerPlex 16
CV: Promega CTTV
CA: Promega CTTA
MP: Promega MonoPlex
Other: Please specify

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