Penta E Variants
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|
Allele Designation |
Allele Size |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
| 5 | 375.63 | ABI 3100 | PPX16 | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-amplified | ||
| 5.1 | 376.3 | ABI 3100/3130XL | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Extraction | ||
| 7.3 | 379.7 | ABI 3500 | PowerPlex Fusion 5C | Tara Scott, Iowa DCI Crime Lab | Re-punched | Convicted Offender | |
|
9.1 |
397.23 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 9.2 | 397.92 | ABI 3100/3130XL | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Extraction | ||
|
9.4 |
399.92 |
ABI 3100 |
PP |
Kelly Carcaise, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 at time of submission |
| 10.1 | 402.02 | ABI 3100 | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Amplification | ||
|
10.2 |
403.09 |
ABI 310 |
PP |
Jackie Johnson,MO State Highway Patrol Crime Lab |
Re-extracted and Re-amplified |
Convicted Offender |
|
| 11.2 | 399.59 | ABI 3500XL | PowerPlex Fusion 6C | Abby Hernandez, Washington State Patrol Crime Lab | Re-Direct Amplification | Convicted Offender | 1 |
|
11.4 |
409.92 |
ABI 3100 |
PP |
Cory Formea.IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 at time of submission |
|
11.4 [2] |
410.02 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| Approximately 11.4 (Slightly >11.4, but <12) | 401.84 & 401.94 | ABI 3500 | PowerPlex Fusion 6C | Ashley Trausch, Saint Louis Metropolitan Police Department Laboratory | Present in reference sample of biological mother and child | 2 | |
|
12.1 |
411.94 |
ABI 3100 |
PP |
Jennifer MacRitchie, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 |
| 12.1 [2] | 403.59 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Convicted offender | |
|
12.2 |
412.90 |
ABI 3100 |
PP |
Cory Formea,IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 at time of submission |
| 12.2 [2] | 404.48 bp | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Convicted offender | |
|
12.3 |
413.8 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 12.4 | 403.76 | ABI 3500 | PP21 | Denise Grover, NT Forensic Biology, Australia | Re-amplification | 1 | |
| 12.4 [2] | 403.69 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-amplified & re-analyzed | Immigration case (AM) | 1 in 2730 |
|
13.2 |
417.92 |
ABI 3100 |
PP |
Cory Formea, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 at time of submission |
|
13.4 |
419.75 |
ABI 310 |
PP |
Cintia Alves, IPATIMUP, Porto, Portugal |
Re-extracted and Re-amplified. Sequenced |
Portuguese Caucasian sample |
1 in 1281 |
| 14.2 | 422.82 | ABI 3130XL | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Amplification, also in multiple samples | ||
| 14.2 [2] | 422.96 | ABI 3100 | PPX16 | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-extracted | ||
| 14.2 [3] | 423.05 | ABI 3100 | PPX16 | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-amplified | ||
| 14.2 | 423.13 | ABI 3100 | PPX16 | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-amplified | ||
|
14.4 |
424.82 |
ABI 3100 |
PP |
Kerry Reavis, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 |
| 14.4 [2] | 416.3 | ABI 3500xl | Promega GenePrint 24 | David Plunkett, Allogen Laboratories, Cleveland Clinic, Cleveland, OH | Re-amplified & re-analyzed | 1 | |
| 14.4 [3] | 416.2 | ABI 3500xl | PowerPlex Fusion 6C | Matthew Fisher, Washington State Patrol Crime Lab | Re-injection | ||
| 14.4 [4] | 416.3 | Spectrum Compact CE, Promega | Promega VersaPlex 27PY | Harutyun Karapetyan & Razmik Sargsyan, Prom-Test LLC, Yerevan, Armenia | Re-amplified and re-analysed | Routine paternity test, two carriers: alleged father and son. | |
|
15.2 |
427.97 |
ABI 3100 |
PP |
Charity Noreuil, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 at time of submission |
|
15.2 [2] |
433.09 |
ABI 377 |
In House Multiplex |
Martin Whittle, Sao Paulo, Brasil |
Re-amplified as a monoplex |
Observed during routine parentage testing, Brazillian caucasian, Mother-child transmission |
1 in 18117 |
|
15.4 |
430.18 |
ABI 3100 |
PP |
Cory Formea, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
3 at time of submission |
| 15.4 [2] | 418.14 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-amplified & re-analyzed | Immigration case (10529 AF + CH) | 2 in 2730 |
| 15.4 [3] | 421.15 | ABI 3500xl | PowerPlex Fusion 6C | Matthew Fisher, Washington State Patrol Crime Laboratory | Re-amplified and re-analyzed | Convicted Offender | 1 |
| 15.4 [4] | 420.82 | ABI 3500 | PowerPlex Fusion 6C | Pamela Larkin, St Louis Metropolitan Police Department Crime Lab | re-amplified | 1 | |
| 16.2 | 433.46 | ABI 3100 | PPX16 | Marsha Garcia, Orchid Cellmark,Nashville TN | Re-amplified | ||
| 16.3 | 422.90 bp | ABI 3500 | Promega PowerPlex Plus | Valeria Marcucci y Claudia Sarapura-Laboratorio Regional de Investigación Forense (Santa Cruz, Argentina) | Re-amplification | father and daughter in a filiation case | |
|
16.4 |
DMRI 432.7 PP 442.8 |
MegaBACE 1000
|
In-house panels PP |
Rita Young & Eric Yap, Defense Medical Research Institute, Singapore |
Initially detected in in-house STR panel, confirmed with PP16 |
DMRI in-house STR panel was verified with commercial stds,GM09947A,GM09948 & K562. Also tested with NIST SRM 2391a |
1 out of 356 alleles for Indians 0 out of 368 alleles forChinese 0 out of 332 alleles for Malay |
|
16.4 [2] |
435.27 |
ABI 130 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
| 16.4 [3] | 435.42 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood Reference | 1 in 11000 |
| 16.4 [4] | 435.35 | ABI 3100 | PP16 | Laura Kuyper M U F S C | Amplification x 2 | 1 | |
| 16.4 [5] | 435.25 | ABI 3500 | PP16 HS | Armando Serra, INML-Coimbra, Portugal | 2 different amplifications | Buccal swab. Portuguese Caucasian sample. | |
| 16.4 [6] | 435.38 | ABI 3130 | PP16 | Kelly Beatty, MUFSC | Confirmed with re-extraction | 3 | |
| 17.2 | 438.41 | ABI 3100 | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Amplification | ||
|
17.4 |
440.59 |
ABI 3100 |
PP |
Kelly Carcaise, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 at time of submission |
| 17.4 [2] | 440.56 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood Reference | 1 in 11000 |
| 17.4 [3] | 427.92 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-amplified & re-analyzed | Immigration case (CH only) | 1 in 2730 |
| 18.1/18.2 | 432.52 | ABI 3500 | PowerPlex Fusion 6C | Krista Spear, St. Louis Metropolitan Police Department Crime Lab | Re-extraction and re-amplification | OL continually sized midway between 18.1 and 18.2 | 1 |
| 18.2 | 443.68 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood Reference | 4 in 11000 |
| 18.2 [2] | 430.73 | ABI 3500 | PowerPlex Fusion system | Dongdong XU, Yun WANG, Huiling YANG, Qingwei FAN, Bing DU. Department of Forensic Medicine of Nor Sichuan Medical College, China | Re-extraction and re-amplification, also sequnenced | Penta E_18.2 [AAAGA]4 AA [AAAGA]14 | 1 |
|
18.4 |
445.94 |
ABI 310 |
PP1 |
Cyndi Hall, Idaho State Police |
Multiple sample sources |
|
1 |
| 18.4 [2] | 431.85 | ABI 3130 | PP21 | Lai Li, Fu jian Academy of Medical Sciences, Fu zhou, China | observed in two individuals | ||
| 18.4 [3] | 435.18 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Convicted offender | |
| 19.2 | 448.83 | ABI 3130XL | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Amplification | ||
|
19.4 |
DMRI 447.8 PP 459.6 |
MegaBACE 1000
|
In-house panels PP |
Rita Young & Eric Yap, Defense Medical Research Institute, Singapore |
Initially detected in in-house STR panel, confirmed with PP16 |
DMRI in-house STR panel was verified with commercial stds,GM09947A,GM09948 & K562. Also tested with NIST SRM 2391a |
0 out of 356 alleles for Indians 1 out of 368 alleles forChinese 0 out of 332 alleles for Malay |
| 19.4 [2] | 439.96 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Convicted offender | |
|
20.2 |
453.95 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 20.2 [2] | 441.73 | ABI 3500 | PowerPlex Fusion 5C | Tara Scott, Iowa DCI Crime Lab | Reran | 1 | |
| 20.4 | 396.04 bp | ABI 3500 | AGCU EX22 made by AGCU Scientech Incorporation | Xiao-Xun Wang; Department of Medical Laboratory, Taihe Hospital Affiliated to Hubei University of Medicine | Re-extract , Re-amplify & re-analyzed | Observed during routine parentage testing, chinese Han ,mother-child transmission | 1 in 5500 |
| 21.2 | 445.64 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-extracted, re-amplified and re-analyzed | Immigration case (AM and CH) | 2:1140 |
| 22.4 | 455.53, 454.82 | ABI 3500 | PowerPlex Fusion 6C | Krista Spear, St. Louis Metropolitan Police Department Crime Lab | Present in two separate reference standards | Reference standards believed to be from related individuals | 2 |
|
23.4 |
470.93 |
ABI 3100 |
PP |
Cory Formea IL State Police, DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 |
| 25 | 240.19 | ABI 310 | AGCU EX22 (made by AGCU SicenTech Inc.) | Wang Xiaoxun, Qi Shaohua, Zhang Jicai, Department of Laboratory Medicine, Affiliated Taihe Hospital, Hubei University of Medicine, China | Re-extracted and re-amplified twice. Sequenced. | Observed during routine parentage testing, Chinese Han | 1/242 |
| 25 [2] | 479.06-479.35 | ABI 3130xl | PowerPlex 18D | Fei Guo, Forensic Science College, Criminal Investigation Police University of China, Shenyang, China | Re-extracted and re-amplified. Sequenced using MiSeq FGx, Penta E_25_[TCTTT]25. | Hui Chinese in a population genetic study | 5 in 1450 alleles |
|
26 |
485.36 |
ABI 377 |
In House Multiplex |
Martin Whittle, Sao Paulo, Brasil |
Re-amplification as a monoplex |
Observed during routing parentage testing, Brazilian caucasian |
1 in 18117 |
| 26 [2] | 468.56 | ABI 3130 | PP21 | Yingai Zhang, Central Laboratory of Haikou People’s Hospital, Haikou, Hainan, China | Re-extracted and Re-amplified, also sequenced | Observed during routine parentage testing, Chinese Han sample | 2 in 726 |
| 26.2 (>24) | 481.93 | ABI 3100 | PP | Laura Kyuper, M U F S C | 1 | ||
| 27 | 473.38 | ABI 3500 | PP21 | Denise Grover, NT Forensic Biology, Australia | Re-amplification | 1 | |
| 27 (>24) | 475.64 | ABI 3500xl | PowerPlex Fusion 6C | Matthew Fisher, Washington State Patrol Crime Lab | Extraction | ||
| 27.4 | 480.53 | ABI 3500 | PowerPlex Fusion 6C | Michelle Hassler San Diego Sheriff's Crime Lab | Re-injection | 1 | |
| 28 | 477.97 | ABI 3500 | PP21 | Denise Grover, NT Forensic Biology, Australia | Re-amplification | 1 | |
| 28.4 | 481.91, 381.92 | ABI 3130 | PP21 | Lai Li, Forensic Sciences Institute of Fujian Provincial Hospital, Fu Zhou, Fujian, China | Observed in father and his daughter | ||
| 32 | 454.28 | 3130XL | AGCU EX22（made by AGCU SicenTech Inc.） | Zhang Shuai, Lu Zhixi, Zhou Jing, Fu Ying, Huang Qiong, Xu Enping. Forensic Science Center of Zhejiang University | Re-extraction, re-amplification with Goldeneye 20A, and sequenced | Observed during routine parentage testing. | 1 |
37 reported variants as of 11/15/2017
Tri-Allelic Patterns
|
Allele Designation |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
|
5, 7, 16 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
7, 8, 14 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
7, 8, 16 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 7,17,18 | ABI 3130 | PP | Silvia Fallas González and María José Pineda Padilla. Laboratorio de Pruebas de Paternidad, Caja Costarricense del Seguro Social | Re-extract and re-amplified | Sample from a paternity case (child) | Observed once |
| 7, 9, 13, 15 |
ABI 3130xl 386.59, 396.60, 415.88, 425.86 |
PP | Josh Corbin, The Genetic Testing Laboratories, Inc. | 2nd independent analysis | observed during routine parentage testing | 1 |
| 9, 16, 17 | ABI 3500 | PowerPlex Fusion 6C | Erik Hall, St. Louis Metropolitan Police Department Crime Lab | Re-extraction | Triallele in reference standard | 1 |
|
11, 12, 14 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
11, 14, 15 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
11, 16, 17 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 12, 13, 22 | ABI 3500XL | Fusion 6C | Matthew Fisher, Washington State Patrol Crime Lab | Re-injection | Type 1 - Allele Size: 402.26, 407.01, 451.19 | |
|
12, 17, 18 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
12, 20, 21 |
ABI 310 |
PP16 | Carlos Vullo, LIDMO-EAAF Forensic Genetic Lab, Cordoba, Argentina | Re-extracted, re-amplified twice | parentage case, pattern observed in alleged father | 1 in 12300 |
| 12, 21, 22 | ABI 3500 | PowerPlex Fusion 6C | Alison P. Walker, Washington State Patrol | Re-extract and re-amplify | Reference sample | observed once |
| 13,14,17 | ABI 3130 | PP | Silvia Fallas González and María José Pineda Padilla. Laboratorio de Pruebas de Paternidad, Caja Costarricense del Seguro Social | Re-extract and re-amplified | Sample from a paternity case (mother) | Observed once |
| 13, 16, 17 | ABI 3100 | PP | ICMP | Re-extraction and amplification | Type 1 | 1 in 11000 |
|
13, 21, 22 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 14, 15, 17 | ABI 3500XL | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-direct amplification | Type 1, Convicted offender | |
|
14, 18, 19 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 15, 16, 17 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-extraction | Type 1, Convicted offender | |
|
16, 17, 18 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
16 reported tri-allelic patterns as of 03/03/2017
* Use the following abbreviations for amplification kit names:
- CO: Applied Biosystems COfiler
- PR: Applied Biosystems Profiler
- PS: Applied Biosystems Profiler Plus
- ID: Applied Biosystems Identifiler
- PP: Promega PowerPlex 16
- CV: Promega CTTV
- CA: Promega CTTA
- MP: Promega MonoPlex
- Other: Please specify