Penta D Variants
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|
Allele Designation |
Allele Size |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
|
1.1 |
363.27 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
|
|
1.2 |
363.56 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
1 in 33739 |
| 3 | 371.6 | ABI 3100 | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Amplification, also in multiple samples | ||
| 3.4 | 374.09 | ABI 3100 | PP | Chromosomal Laboratories, Inc. Phoenix, AZ | Observed in parent and two children | Paternally transferred allele | 3 |
|
4 |
375.79 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
|
| 4 [2] | 383.63 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-amplified & re-analyzed | Immigration case (10720) | 2 in 2730 |
| 4.1 | 377.26 | ABI 3100 | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Extraction | ||
| 4.2 | 386.39 | ABI 3500 | PP21 | François Chatenay-Lapointe, Rhode Island Blood Center | Re-amplified and Re-analyzed | Immigration case. | 1 in 3830 |
| 5.4 | 384.00 | ABI 3130 | PP | Stacy Bozinovski, Indiana State Police Laboratory | Re-amplification | ||
|
6 |
386.23 |
ABI 3100 |
PP |
Charity Noreuil IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender |
multiple times |
| 6 [2] | 385.02 | ABI 3130xl |
PP
|
Justin Godgy M U F S C | 4 | ||
| 6 [3] | 385.14 | ABI 3130xl |
PP
|
Justin Godgy M U F S C | 5 | ||
| 6 [4] | 385.05 | ABI 3130xl |
PP
|
Justin Godgy M U F S C | 6 | ||
| 6 [5] | 384.92 | ABI 3130xl |
PP
|
Nicole Hoffman M U F S C | 7 | ||
| 6 [6] | 384.97 | ABI 3130xl |
PP
|
Nicole Hoffman M U F S C | 8 | ||
| 6 [7] | 384.61 | ABI 3130 | PP16HS | Nicole Stickle, Indiana State Police | Technical review, administrative review | hair sample from sweatshirt in a burglary case, no standard to compare it to | once |
|
6.4 |
390.14 |
ABI 3100 |
PP |
Kelly Carcaise IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 at time of submission |
|
6.4 [2] |
389.49 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
| 6.4 [3] | 397.92 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-amplified & re-analyzed | Immigration case (CH) | 1 in 2730 |
|
7.1 |
392.13 |
ABI 3100 |
PP |
Charity Noreuil, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 |
|
7.4 |
395.10 |
ABI 3100 |
PP |
Jennifer MacRitchie, IL State Police DNA Indexing Lab |
Re-amplification |
Conficted Offender Sample |
1 |
|
7.4 [2] |
394.21 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
| 7.4 [3] | 393.92 | ABI 3100 | PP 16 | Laura Kuyper M U F S C | Amplified twice with PP 16 | ||
| 8.1 | 395.32 395.19 395.25 | ABI 310 | PP | Paul Gilbert, State of Deleware, OCME | Re-amplified and re-analyzed | Observed in both reference and evidentiary samples | |
| 8.1 [2] | 408.43 | ABI 3500XL | Fusion 6C | Matthew Fisher, Washington State Patrol Crime Lab | Re-direct amplification | ||
| 8.2 | 396.81 | ABI 3130 | PP | Nicole Hoffman M U F S C | 1 | ||
| 8.2 [2] | 396.83 | ABI 3130xl | PP | Nicole Hoffman M U F S C | 2 | ||
| 8.4 | 399.8 | ABI 3100/3130XL | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Extraction | ||
|
9.1 |
400.98 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
| 9.2 | 402.52 | ABI 3100/3130XL | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Amplification, also in multiple samples | ||
| 9.2 [2] | 401.65 | ABI 310 | PP | Maria Miozzo, Mariana Maxzud, Carla Pacharoni, Silvia Mutal, Nidia Modesti, CEPROCO Argentina | Sequenced | Population of Cordoba Province from Argentina. Published in J. Forensic Sci 2007;52:1073-1076 | |
| 9.2 [3] | 401.29 | ABI 3100 | PP | Cantagalli, V.D.; Matsuzaki, L.N.; Coudry,R.A.; Fleury - Medicina e Saude | Re-extraction and Re-amplification (two samples from the same patient) | Paternity Samples | 1 in 7199 |
|
9.4 |
404.70 |
ABI 3100 |
PP |
Charity Noreuil IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender |
|
| 9.4 [2] | 402.24 | ABI 3130 | PP | Brandt Cassidy, DNA Solutions, Inc, Oklahoma City OK | Re-isolate and re-amp | Excluded paternity case | 1 in 4000 |
|
10.1 |
405.85 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
|
| 10.1 [2] | 418.41 bp | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Convicted offender | |
|
10.2 |
406.59 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
|
| 10.2 [2] | 419.36 bp | ABI 3500XL | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Convicted offender | |
|
10.3 |
408.51 |
ABI 3100 |
PP |
Cory Formea IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
2 at time of submission |
|
11.1 |
411.22 |
ABI 3100 |
PP |
Kerry Reavis IL State Police Indexing Lab |
Re-amplified |
Convicted Offender Sample |
1 at time of submission |
|
11.1 [2] |
410.47 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
|
11.2 |
412.16 |
ABI 3100 |
PP |
Kelly Carcaise IL State Police DNA Indexing Lab |
Re-Amplified |
Convicted Offender Sample |
1 at time of submission |
|
11.2 [2] |
412.13 |
ABI 310 |
PP |
Angelo ABATI, Institut de Medecine Legale de Liege Belgium |
3 amplifications |
Convicted Offender |
1 in 600 |
| 11.2 [3] | 412.08 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood reference | 1 in 11000 |
| 11.3 | 412.88 | ABI 3130XL | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Extraction | ||
| 11.3 [2] | 424.81 | ABI 3500 | PowerPlex Fusion 6C | Kaylene Folks, Washington State Patrol Crime Laboratory | re-injected | 1 | |
| 11.4 | 414.15 | ABI 3100/3130XL | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Extraction | ||
| 11.4 [2] | 426.51 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-direct amp | Convicted Offender | |
|
12.1 |
415.17 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
|
| 12.1 [2] | 416.13 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood reference | 18 in 11000 |
|
12.2 |
417.37 |
ABI 3100 |
PP |
Cory Formea IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 at time of submission |
|
12.2 [2] |
416.27 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
| 12.2 [3] | 416.74 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood reference | 1 in 11000 |
| 12.2 [4] | 416.19 | ABI 3130 | PP | Justin Godby M U F S C | 1 | ||
|
12.3 |
417.25 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
|
|
12.4 |
418.77 |
ABI 3100 |
PP |
Kelly Carcaise IL State Police DNA Indexing Lab |
Re-Amplified |
Convicted Offender Sample |
1 at time of submission |
| 12.4 [2] | 431.83 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-direct amp | Convicted offender | |
| 13.1 | 429.13 | ABI 3500xl | Verifiler Plus | Simon Knights – Anglia DNA Services | Re-amplification and re-run on capillary electrophoresis | Paternal allele (father unidentified but not present in mother) | 1 |
|
13.2 |
421.95 |
ABI 3100 |
PP |
Charity Noreuil, IL State Police DNA Indexing Lab |
Re-amplification |
|
|
|
13.3 |
421.83 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
13.4 |
423.79 |
ABI 3100 |
PP |
Cory Formea, IL State Police, DNA Indexing Lab |
Re-amplification |
Convicted Offender |
1 at time of submission
|
|
13.4 [2] |
422.85 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
| 13.4 [3] | 422.66 | ABI 3130xl | PP | Justin Godgy M U F S C | 2 | ||
| 13.4 [4] | 422.67 | ABI 3130 | PP |
Peter M. Schneider Institute of Legal Medicine University of Cologne |
Repeated extraction & analysis using PP16 kit
|
Sibling case, variant allele not shared by putative brothers
|
quite low ... |
| 13.4 [5] | 436.74 bp | ABI 3500XL | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-amplification | Convicted offender | |
|
14.1 |
425.86 |
ABI 3100 |
PP |
Kerry Reavis, IL State Police, DNA Indexing Lab |
Re-amplification |
Convicted Offender |
1 at time of submission
|
|
14.1 [2] |
424.65 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
|
14.1 [3] |
424.57 |
ABI 310 |
PP |
DNA Solutions |
Reisolation and PCR |
Passed from Father to Child |
1 in 1598 |
| 14.1 [4] | 424.56 | ABI 3100 | PP 16 | Laura Kuyper M U F S C | Re-amplification s 1 | 1 | |
| 14.1 [5] | 425.76 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood reference | 1 in 11000 |
|
14.4 |
429.05 |
ABI 3100 |
PP |
Charity Noreuil, IL State Police DNA Indexing Lab |
Re-Amplified |
Convicted Offender Sample |
1 at time of submission |
|
14.4 [2] |
429..03 |
ABI 3100 |
PP |
Jaclyn Garfinkle, IL Sate Police DNA Indexing Lab |
Re-amplified |
Convicted Offender |
1 |
|
14.4 [3] |
427.93 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
|
15.1 |
429.84 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol Crime Lab |
Re-extract and Re-amplify |
Convicted Offender |
|
| 15.4 | 433.29 | ABI 3130XL | PP | Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory | Re-Amplification | ||
| 18 | 444.52 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood reference | 12 in 11000 |
| 18 [2] | 455.59 | ABI 3500 | PP21 | Francois Chatenay-Lapointe, Rhode Island Blood Center | Re-amplified and re-analyzed | Immigration case | 1 in 3266 |
|
18 (>17) |
443.76 |
ABI 310 |
PP |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
| 19 | 449.50 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood reference | 1 in 11000 |
| 19 [2] | 463.34 | ABI 3500 | PowerPlex Fusion 6C | Erik Hall, St. Louis Metropolitan Police Department Crime Lab | Re-amplification | 1 | |
| 19.4 | 466.38 | ABI 3130xl | PowerPlex Fusion 6C | Carlos Flores, Alliance DNA Laboratory | Re-Amplification | Immigration case | 1 |
| 21 | 466.7 | ABI 3130 | Huaxia Platinum | Yingai Zhang, Xiaohong Wen. Haikou people’s Hospital, Central Laboratory, Haikou, China | Re-extraction, re-amplification with Goldeneye 20A, and sequenced on an Illumina nextseq 500. | sample from a paternity case (mother and daughter) | 1 |
| 22 (>17) | 473.25 bp | ABI 3500XL | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-direct amplification | Convicted offender |
38 reported variants as of 12/30/2016
Tri-Allelic Patterns
|
Allele Designation |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
| 2.2, 6, 9 | ABI 3130 | PP16 HS | Silvia Fallas Gonzalez and Maria Jose Pineda Padilla, Laboratorio de Pruebas de Paternidad, Caja Costarricense del Seguro Social | Re-amplified | sample from a paternity case (child) | 1 |
| 2.2, 10, 11 | ABI 3130 | PP 16 | Caribbean Genetics | Duplicated | Paternity Sample | 1 |
| 8, 9, 13 | ABI 3500 | PowerPlex Fusion system and AGCU EX22 | Dongdong XU, Yun WANG, Huiling YANG, Bing DU. Department of Forensic Medicine of North Sichuan Medical College, China | Re-extraction, re-amplification with PowerPlex Fusion system, confirmation with AGCU EX22 (made by AGCU SicenTech Inc.) | Paternity Sample( son), Type 2 | 1 |
| 8, 10, 12 | ABI 3100 | PP | Sun Hongyu, Chen Wenjing, Xue Tianyu. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China | Re-extraction, re-amplification with PP16, confirmation with singleplex amplification | Paternity Samples, Type 2 | 1 in 28252 |
|
8, 10, 13 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
| 9, 10, 10 | ABI 3100 | PP | ICMP | Re-extraction and amplification | "Double dose" effect with the 10 peak approx. twice the height of the 9, Same sample was triallelic at D21S11 | 1 in 11000 |
| 9, 10, 12 | ABI 3100 | PP | Sun Hongyu, Chen Wenjing, Xue Tianyu. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China | Re-extraction, re-amplification with PP16, confirmation with singleplex amplification | Paternity Samples, Type 2 | 1 in 28252 |
|
9, 10, 13 |
ABI 310 |
PP |
Rebecca Wallman, State of Delaware OCME |
Re-amplified and re-analyzed |
Observed in both known and evidentiary samples |
1 |
| 9, 11, 12 | ABI 3100 | PP | Sun Hongyu, Chen Wenjing, Xue Tianyu. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China | Re-extraction, re-amplification with PP16, confirmation with singleplex amplification | Paternity Samples, Type 2 | 1 in 28252 |
|
9, 12, 13 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
| 9, 12, 14 | ABI 310 | PP | Elaine Taylor, Oklahoma City Police Department | Duplicated - pattern seen in two question samples and known reference | ||
| 10, 11, 12 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-direct amplification | Convicted offender, Type 1 | |
| 10, 11, 13 | ABI 3500xl | PowerPlex Fusion 6C | Matthew Fisher, Washington State Patrol Crime Laboratory | Re-amplified and re-analyzed | Allele Size: 417.24, 422.30, 432.55 - Convicted Offender | 1 |
| 10, 11, 15 | ABI 3500 | PowerPlex Fusion 5C | Tara Scott, Iowa DCI Crime Lab | Re-punched | Convicted Offender | |
|
10, 12, 13 |
ABI 310 |
PP |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
| 10, 13, 14 | ABI 3100 | PP | Sun Hongyu, Chen Wenjing, Xue Tianyu. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China | Re-extraction, re-amplification with PP16, confirmation with singleplex amplification | Paternity Samples, Type 2 | 1 in 28252 |
| 11, 12, 13 | ABI 3500xl | PowerPlex Fusion 6C | Sarah Ellis North Carolina State Crime Laboratory | re-amplification | Reference Sample - Allele Size: (2956, 1316, 766 on first run and 2554, 1245, 727 on re-amp) | 1 |
| 12, 13, 14 | ABI 3500XL | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Type 1, Convicted offender | |
12 reported tri-allelic patterns as of 12/30/2016
* Use the following abbreviations for amplification kit names:
- CO: Applied Biosystems COfiler
- PR: Applied Biosystems Profiler
- PS: Applied Biosystems Profiler Plus
- ID: Applied Biosystems Identifiler
- PP: Promega PowerPlex 16
- CV: Promega CTTV
- CA: Promega CTTA
- MP: Promega MonoPlex
- Other: Please specify
Last Updated: 12/30/2016