Page last updated: 03/17/2023
D2S441 Variants
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|
Allele Designation |
Allele Size |
Instrument |
Amp Kit* |
Contributor |
Verification/ Confirmation Method(s) |
Notes |
Frequency |
| 5 (< 9) | 199.91 | ABI 3500XL | PowerPlex Fusion 6C | Abby Hernandez, Washington State Patrol Crime Lab | Re-Injection | Convicted Offender | 1 |
| 6 (<8) | ABI 3500xl | PowerPlex Fusion, GlobalFiler | Rhonda Williams, Oklahoma State Bureau of Investigation | Re-punched and analyzed in another amplification system | 2 | ||
| 7 (<9) | 208.18 | ABI 3500xl | PowerPlex Fusion 6C | Matthew Fisher, Washington State Patrol Crime Lab | Re-direct amplification | convicted offender | |
| 7 (<8) [2] | Fusion: 208.78 & 208.61 GlobalFiler: 72.59 | ABI 3500 | PowerPlex Fusion 5C & GlobalFiler | Tara Scott, Iowa DCI Crime Lab | Confirmed by repunches in PPF 5C & GlobalFiler | 1 | <8 | 84.30 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Crime scene sample | 1 (first observation) |
| <8 [2] (called as a 20.3 allele in D1S1656; most likely contains 7 D2S441 repeats) |
204.89 | ABI 3500xl | PowerPlex Fusion | Alyse Yacovone-Margetts, Palm Beach County Sheriff's Office | Utilized the allelic ladder to determine the base pair difference between the called allele in D1S1656 and the smallest allele, from the allelic ladder, in D2S441. The sample was not reamplified or reinjected, however the same pattern was observed in evidence samples. | This allele was called as a 20.3 microvariant within the D1S1656 locus. However, based on the fact that the locus already was called a heterozygote (14,16) and that tri-allelic patterns usually do not have a wide a repeat range as this would have been, it was investigated to see if this allele could be smaller than the smallest allele in the Fusion kit for D2S441. Using an allelic ladder, it was determined that the called peak of 20.3, 204.89 bp was exactly 4.02 bp smaller than the smallest allele (8; 208.91 bp) in the kit allelic ladder for this marker. | 1 |
| 8 | 76.34 | ABI 3130xl | NGM |
Nelson Gaburo Junior, Luciano Cesar Scarpelli, Daniela Moratori, Pamela de Melo Machado, Carla Naufal Fontes, Renato Bueno de Moraes, Maria Cristina Sales, Tamyrez Ayrez Marques - Department of Molecular Biology – Diagnósticos da América - DASA |
Re-amplified and re-analyzed | Observed in paternity identification | |
| 8.1 | 89.18 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
| 9.1 | 346 (ESI 17) 81 (NGM SElect) |
ABI 3130xl | NGM SElect and PowerPlex ESI 17 | M.llo De Meo RIS ROMA | |||
| 9.1 [2] | 93.30 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Crime scene sample | 1 (first observation) |
| 9.3 | 349.26 | ABI 3500 | ESI kit | Margalit Sin David, Israel Police DNA Database, Jerusalem, Israel | Re-extracted and re-amplified | ||
| 9.3 [2] | 95.58 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
| 9.3 [3] | 83.9 | ABI 3500xl | Verifiler Plus | Simon Knights & William Stevenitt – Anglia DNA Services | Re-amplification and re-run on capillary electrophoresis | Both Father and Daughter share the variant allele | 2 |
| 9.3 [4] | 218.57 | ABI 3500XL | Fusion 6C | Matthew Fisher, Washington State Patrol Crime Lab | Re-injection | ||
| 10.1 | 351.42 | ABI 3500xl | ESI | Sivan Mishan, Liron Elkayam, Carla Oz, Israel Police DNA Database | Re-extracted and re-amplified | reported size is average of 251.44 bp and 351.40 bp | 2 samples |
| 10.1 [2] | 97.47 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
| 10.1 [3] | 86.14 | ABI 3500 | Applied Biosystems GlobalFiler | Hortensia Cano y Valeria Marcucci- Laboratorio Regional de Investigación Forense- Santa Cruz- Argentina | Re-amplification with PowerPlex Fusion-Promega (Allele Size: 217.84) | sample of reference and evidence of the same judicial cause | 1 (First observation) |
| 10.1 [4] | 86 | ABI3130xl | Globalfiler | Asmer Aliyeva, Victoria Gellatly, Arati Iyengar - University at Albany, SUNY, USA | Reamplified and confirmed | 1/934 | |
| 10.2 | 94.20 (ESX); 353.04 (ESI) | ABI 3500 | Promega PowerPlex ESX17 Fast, Promega PowerPlex ESI17 Fast | Department of Forensic Genetics, University Medical Center Schleswig-Holstein, Germany | Re-extracted and amplified | National DNA database sample | 1 |
| 10.3 | 220.60 | ABI 3500 | PowerPlex Fusion |
Paola Zanelli, Stefania Bardini, Silvia Giuliodori, Magda Benecchi, Valentina Sgobba – Immunogenetics Laboratory, Parma University Hospital |
Re-extracted and re-amplified | ||
| 10.3 [2] | 99.47 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Crime scene sample | 1 (first observation) |
| 10.3 [3] | 222.89 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-direct amplification | Convicted offender | |
| 10.3 [4] | 88.19 | ABI 3500 | GlobalFiler | Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section | re amplified | Convicted Offender | 1 |
| 11.1 | 101.31 | ABI 3500 | PowerPlex ESX 17 and Fusion |
Docteur Angelo ABATI Responsable du Laboratoire d'identification génétique de l'Institut de Médecine Légale de l'Université de Liège, Belgium |
2 extracts each of them 2 times with the 2 kits | 1 individual | |
| 11.1 [2] | 101.61 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
| 11.1 [3] | 255.64 bp | ABI 3500XL | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Convicted offender | |
| 11.1 [4] | 90.08 and 90.34 | ABI 3500 | Globalfiler | Crystal Beepot - Institute of Forensic Science and Legal Medicine | Seen in two samples | 2 | |
| 11.1 [5] | 222 bp (221.4 bp) | Spectrum Compact CE System (Promega) | Promega PowerPlex Fusion | Laboratorio de Diagnóstico Molecular, Argentina. | Reextraction, reamplification, reinjection. | Reference sample | 1 (first time) |
| 11.2 | 103.17 | ABI 3500xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
| 11.3 | 463.49 bp | ABI 3500XL | AGCU EX22 made by AGCU Scientech Incorporation | Xiao-Xun Wang; Department of Medical Laboratory, Taihe Hospital Affiliated to Hubei University of Medicine | Re-extract , Re-amplify & re-analyzed | Observed during routine parentage testing, chinese Han ,father-child transmission | 1 in 5500 |
| 12.1 | 105.78 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
| 12.3 | 235.75 | ABI 3500xl | ESI | Sivan Paz, Carla Oz, Israel Police DNA Database | Re-extracted and re-amplified | reported size is average of 235.78 bp and 235.71 bp | 1 sample |
| 12.3 [2] | 94.03 | ABI 3500 | NGM SElect | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender sample | |
| OLA | 96.6 | ABI 3500XL | GlobalFiler Express (GFE) | DFSC | Re-amp and/or Re-extraction (sample-type dependent) | Multiple observations of alleles falling between bins. Some migration in and out of 12.3 or 13 bin. Nothing else unusual identified in the associated electropherograms. | 5 times. Once in 2018, once in 2019, twice in 2020, and once so far in 2021. |
| 13.1 | 109.91 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Crime scene sample | 1 (first observation) |
| 13.1 [2] | 98.38 & 98.35 | ABI 3500 | GlobalFiler | Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section | re amplified | Question Sample, Known Sample & Convicted Offender | 3 |
| 14.1 | 113.61 | ABI 3500xl | ESX17 (Promega) | Christophe Frippiat, National Institute of Criminalistics and Criminology, Belgium | re-amplified with kit ESI17Pro (size of 367.09) | 1 individual | |
| 14.1 [2] | 238.3 | ABI 3500 | PowerPlex Fusion 6C | Erik Hall, St. Louis Metropolitan Police Department Crime Lab | Multiple samples | 2 | |
| 14.1 [3] | 238.17 | ABI 3500 | PowerPlex Fusion 6C | Erica Mpoy, St. Louis Metropolitan Police Department Crime Lab | Reamplified | Variant was seen in the an evidence sample | 1 |
| 14.1 [4] | 102.45 | ABI 3130 | GlobalFiler | Observed in multiple samples (crime scene sample and known suspect) and confirmed by re-amplification | Observed from one individual | ||
| 14.1 [5] | 238.16, 238.12, 238.08, 238.13 | ABI 3500 | PowerPlex Fusion 6C | Amy Rathmann, St. Louis Metropolitan Police Department Crime Lab | Observed in multiple samples from same case | 4 | |
| 14.1 [6] | 102.4 | ABI 3500 | GlobalFiler | Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section | re amplified | Convicted Offender | 1 |
| 14.1 [7] | 237.86, 237.85, 237.33 | ABI 3500xl | PowerPlex Fusion 6C | Kathryn Hoodenpyle St. Louis Metropolitan Police Department Crime Laboratory | Observed in two unknown samples and one reference standard from the same case | 3 | |
| 14.1 [8] | 238.03 | ABI 3500 | PowerPlex Fusion 6C | Karen Preiter, Ph.D. St. Louis Metropolitan Police Dept. Crime Lab | seen in 7 separate evidence samples | 7 samples | |
| 14.1 [9] | 234.1, 233.97, 234.09, 233.98, 234.08, 234.17, 234.13 | ABI 3130xl | PowerPlex Fusion 5C | Megan May, Kentucky State Police Central Forensic Lab | Re-injection | present in 2 unknown samples and a standard/duplicate | 4 |
| 14.2 | 114.10 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
| 16.1 | 246.68 | ABI 3500 | PowerPlex Fusion 6C | Karen Preiter, St. Louis Metro Police Dept, St. Louis, MO | re-analyzed and re-amplified | 1 in 183,186 | |
| 17.2 (>16) | ABI 3500 | PowerPlex Fusion 6C | Erica Mpoy, St. Louis Metropolitan Police Department Crime Lab | Re-amplified | Observed in multiple samples | 2 | |
| 17.2 [2] | 251.04, 250.75 | ABI 3500 | PowerPlex Fusion 6C | Erica Mpoy, St. Louis Metropolitan Police Department Crime Lab | Reamplified | Evidence Sample | 2 |
13 reported variants as of 08/04/2016
Tri-Allelic Patterns
|
Allele Designation
|
Instrument
|
AMP KIT
|
Contributor
|
Verification/Conformation Method(s)
|
Notes
|
Frequency
|
| 8, 10, 14 | ABI 3500 | Promega Fusion 5C | Megan O’Donnell, New Hampshire State Police Forensic Laboratory | Reprep, Reamp, Reanalyze | Convicted Offender Sample | 1 |
| 10, 11, 12 | ABI 3500 | GlobalFiler | Reuben Berdugo, Head of the genomic DNA laboratory of the Israel Defense Forces | Allele Size: 85.09(10), 89.19(11), 93.31(12) - observed in multiple samples | ||
| 10, 12, 14 | ABI 3500 | GlobalFiler | Michael Schmit, Iowa DCI Criminalistics Laboratory | re-amplified | Reference sample | 1 |
| 11, 12, 13 | ABI 3500 | GlobalFiler | Therese Gibler, Sedgwick County Regional Forensic Science Center | Re-extract | Tri-allelic pattern observed in buccal standards, but only 11,12 observed in evidentiary hair sample. Allele Size: 89.08 (11), 93.17 (12), 97.28 (13) | 1 individual |
1 reported tri-allelic patterns as of 11/29/2017
* Use the following abbreviations for amplification kit names:
§ CO: PE Applied Biosystems COfilerTM
§ PR: PE Applied Biosystems ProfilerTM
§ PS: PE Applied Biosystems Profiler PlusTM
§ ID: PE Applied Biosystems Indetifiler™
§ CV: Promega CTTVTM
§ CA: Promega CTTATM
§ PP: Promega PowerPlexTM
§ MP: Promega MonoPlexTM
- Other: Please specify