Page last updated: 03/17/2023

D2S1338 Variants

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Allele Designation

Allele Size

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency
10 220.15 ABI 3500 PowerPlex 21 Francois Chatenay-Lapointe, Rhode Island Blood  Center Re-amplified and re-analyzed Immigration case; Race: Black 2 in 2947

11

290.61

ABI 310

ID

Cintia alves, IPATIMUP, Porto, Portugal

Re-extracted and re-amplified

Portuguese Caucasian sample

1 in 780
11 [2] 289.88 ABI 3500 ID, NGM, and in-house assay Eduardo Leal Rodrigues, Laboratório Centro de Genomas

Re-extracted and re-amplified by using two different amplification kits

 This allele has been localized on the analysis range of D16S539 locus between the bins of allele 14 and 15. The in-house kit contains only the D16S539 and the alleged third allele was not found.  
11 [3] 288.83 ABI 3130 ID Plus Ethan Smith, Washington State Patrol Crime Lab, Spokane, WA Re-extracted and re-amplified Initially thought to be a D16S539 "14.2" (part of a 11,12,14.2 tri-allele)  
11.3 294.85 ABI 3130xl Identifiler Brian Murphy and Meegan Fitzpatrick, NYSP Forensic Investigation Center Re-amplified with COfiler    
12 294.21 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN Re-extracted    
12 [2] 294.31 ABI 3130XL ID Genetica, DNA Laboratories, Inc., Cincinnati OH Inheritance (found in Child & Father)    
12 [3] 294.73 & 108.66 ABI 310 ID & MiniFiler Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Re-amplified and re-analyzed Convicted offender sample  
12 [4] 294.99 ABI 3500 Identifiler Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Re-amplified and re-analyzed Convicted offender sample  
12 [5] 293.92 ABI 3500 ID, NGM, and in-house assay Eduardo Leal Rodrigues, Laboratório Centro de Genomas

Re-extracted and re-amplified by using two different amplification kits

 This allele has been localized on the analysis range of D16S539 locus between the bins of allele 14 and 15. The in-house kit contains only the D16S539 and the alleged third allele was not found.  
12 [6] 293.39 (ID Direct), 106.47 (MiniFiler) ABI 3500xl Identifiler Direct & MiniFiler Arizona Department of Public Safety DNA Database Unit Initially run with Identifiler Direct, then verified with MiniFiler   Twice that we can recall
12.3 298.57 ABI 3130 XL ID Genetica, DNA Laboratories, Inc., Cincinnati OH Inheritance (child and father) Also ran with Powerplex 16 to verify that D16 is heterozygous, not trisomy with variant allele. D16 and D2 are next to one another on Identifilier  
12.3 [2] 298.15 ABI 3130 ID Korie Faber, CA DOJ, Fresno Observed in multiple samples The variant peak was filtered out of GeneMapperID because the peak fell in between D16 and D2; criminal case sample without suspect  
12.3 [3] 298.67 ABI 3130xl ID Erik Hall, St. Louis Metropolitan Police Department Crime Lab Seen in multiple samples Not tested with another kit to exclude tri-allele at D16 1

13

282

ABI 310

SGM +

Institute of Forensic Medicine Medical University of Wroclaw, Poland

Re-amplified

Polish Lower Silesian Population

1 in 600

13 [2]

282.31

ABI 3100

SGM +

Faruk Asicioglu

Re-extracted and re-amplified

 

1

13 [3]

282

ABI 310

SGM+

Department of Forensic Medicine, Medical University of Bialystok, Poland

Re-extracted and re-amplified

 

Polish Tatars ethnic group living in Podlasie (NE Poland)

1 in 200

13 [4]

298.15

ABI 3100

ID

Laure Delpech, Police Forensic Laboratory of Marseilles

Re-amplification with SGM+

 

1 in 3000
13 [5] 298.32 ABI 3130XL ID Genetica, DNA Laboratories, Inc., Cincinnati OH Inheritance (found in Mother & Child)    
13 [6] 282.3 ABI 3130 SGM+ Gregor Kljucevsek, Ministry of the Interior, Forensic Science Centre,Slovenia Re-extracted and re-amplified Observed in the oral reference of this individual and case sample 6 in 16000
13 [7] 297.57 ABI 3500 ID Registro Nacional ADN, Servicio Medico Legal, Chile Re-extracted and re-amplified FTA 1
13 [8] 297.39 ABI 3500 ID Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Reamplified and reanalyzed Convicted offender sample  
13 (<15) [9] 298.11 ABI 3130xl ID Ashley Trausch, Saint Louis Metropolitan Police Department Re-amplified The variant peak was filtered out of GeneMapperID because the peak fell in between D16 and D2. 1
13 [10] 298.39 ABI 3130 ID Pamela Calogero, St. Louis Metropolitan Police Department Crime Lab Re-amplified Reference standard 1
13 [11] 232.41 ABI 3500 PP21 Massimo Mangiola, Rhode Island Blood Center Re-amplified & re-analyzed Immigration case (four CH in same family) 4 in 2730
13 [12]280.75ABI 3500NGM SElectEla Zaimi, Institute of Scietific Police AlbaniaRe-amplified and re-analysedThe peak fell in between D16 and D2S13381
14 302.50 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN Re-extracted    
14 [2] 303.85 ABI 310 ID Demeter József Szabolcs, Popescu Octavian, Molecular Biology Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes Bolyai University Re-extracted and re-amplified   1 of 424
14 [3] 303.00 ABI 310 ID Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Re-amplified and re-analyzed Criminal case sample without suspect  
14 [4] 284.96 ABI 3500 NGM SElect Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Re-amplified and re-analyzed Convicted offender sample  
14 [5] 303.44 ABI 3130xl ID Aqeela Abuidrees, General Directorate of Forensic Science Evidence, Bahrain Re-amplification the variant was observed in two different samples from the same crime scene and from three different positions for unknown contributor in one profile so far
<14 ~298.75 ABI 3130 ID Amber Sage, Los Angeles County Sheriff's Department re-injection, also observed in forensic unknowns Observed in the oral reference of this individual and in four forensic unknowns 5
<15 294.4

294.55

 ABI 3130 ID & CO Karen Galindo, Suffolk County Crime Lab, NY Cofiler -  amplification re-injected CoFiler Amplification - Heterozygote not triallelic at D16 locus 1
<15 (12) 294.35 ABI 3130 &

3130XL

 ID & CO James Holmes, Arizona Department of Public Safety Crime Laboratory Re-amplified and reanalyzed The convicted offender sample was from the Arizona Department of Public Safety Crime Laboratory. The variant peak is in the designated D16 locus as defined in the ID allelic ladder and is labeled as an "OL" alle,(triallele). However, after further review, the sample was re-amplified using COfiler kit. The CO- filer kit confirmed the variant allele belonged to the D2S1338 locus 1
<15 (12) [2] 294.53 &108.82 ABI 310 ID & (MiniFiler) Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Re-amplified and re-analyzed Convicted offender sample from the Forensic Genetics Department, Autonomous Police of the Basque Country, The variant peak is in the D16S539 locus as defined in the Identifilier allelic ladder and is labeled as an "OL" allele (Triallele pattern). Amplifying the sample with the MiniFiler kit confirmed that the variant belonged to the D2S1338 locus.  
<15 (12) [3] 293.54 ABI 3500 ID Plus Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Re-amplified and re-analyzed Convicted offender sample  
<15 (12) [4] 293.49 ABI 3500 ID Plus Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country Re-amplified and re-analyzed Convicted offender sample  

<15 (13)

298.30

ABI 3100

ID & CO

Angela Withrow

M U F S C

Re-amp x 1 each system

This is a convicted offender sample from Alabama Department of Forensic Sciences. The variant peak was filtered out in Genotyper because it didn't know which locus to assign. The Peak fell in the middle of D16 and D2 in the Identifiler amp. Amplifying the sample with Cofiler confirmed that the variant belonged to the D2S1338 locus.

1 in 15543
17.2 306.96 (GlobalFiler), 315.09 (Identifiler) ABI 3500xl ID & GlobalFiler Sandrine Boedec, Kabile Fabrice, Sylvain Hubac, National Forensic Science Institute of the French Gendarmerie, IRCGN, Pontoise, France male reference sample on FTA (buccal cells) re-extracted and re-amplified by using two differents amplification kits D2S1338 heterozygous (17.2;20) 1 in 649,022
18.1 321.88 ± 0.02 ABI 3130xl ID Plus Paulo Dario, Isabel Lucas, Catarina Dourado and Teresa Ribeiro, National Institute of Legal Medicine and Forensic Sciences – South Branch, Portugal Inheritance (found in Mother & 2 Children)   3
18.3 322.32 & 322.41 ABI 3130xl ID Laurent Cardin & Frederic G. Brard, IRCGN - National Forensic Institute of the French Gerdarverie, Rosny-sous-Bois, France Re-extraction and re-amplification FTA Card - Saliva 1/85000
19.1 326.09 (Identitilier)

137.91 (MiniFiler)

 ABI 310 ID & MiniFilier Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country Re-amplified and re-analyzed Convicted offender sample from the Forensic Genetics Department, Autonomous Police of the Basque Country  
19.1 [2] 324.81-325.10 ABI 3130xl ID

Guillaume Boutrois, Francis Hermitte, and Frederic Brard,

 National Forensic Science Institute of the French Gendarmerie. IRCGN, Rosny-sous-Bois, France

 Extracted and amplified twice separately Female reference sample on FTA (buccal cells);

 genotype: 19.1, 23

 1 in 70,000

19.3

325.84

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500
20.1 328.5 ABI 3130XL ID Genetica, DNA Laboratories, Inc., Cincinnati OH Inheritance (found in Child & Father)    
20.3 332.02 ABI 310 ID Bethany L. Harris - Acadiana Criminalistics Laboratory (New Iberia, LA) Re-amped sample using ID to confirm Suspect reference sample  
20.3 [2] 234.41 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Crime scene sample 1 (first observation)
22.1326.84ABI 3500GlobalFilerJunmarie Soto, Iowa DCI Criminalistics LaboratoryRe-amplification and re-analysisCasework sample1
22.2 338.35 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville  TN re-extracted, observed in multiple samples    
22.2 [2] 338.24-338.67 ABI 3130xl ID Francis Hermitte, Marie-Beatrice Tonanny, Sylvain Hubac and Frederic Brard, National Forensic Science Institute of the French Gendarmerie, IRCGN, Rosny-sous-Bois, France male reference sample on FTA (buccal cells) extracted and amplified twice separately D2S1338 heterozygous (21,22.2) 1/60,000
22.3 338.96 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN Re-amplified    

23.2

341.72

ABI 3100

ID

Angela Withrow

M U F S C

Re-amplified

Alabama Dept of Forensic Sciences Convicted Offender

4 in 14,544

23.2 [2]

341.48

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500
23.2 [3] 342.57 ABI 310 ID Nicolas Ronquillo TXDPS      El Paso Found in known reference sample also (342.6)    
23.2 [4] 341.85 ABI 3100 ID Angela Withrow     M U F S C      
23.2 [5] 342.03 ABI 3100 ID Angela Withrow     M U F S C      
23.2 [6] 341.72 ABI 3100 ID Angela Withrow     M U F S C      
23.2 [7] 343.14 ABI 3130 ID Cintia Fridman, Department of Legal Medicine, University of Sao Paulo, Brazil Re-extraction and re-amplification Project of Missing Kids"Caminho de Volta" 1 in 650
23.2 [8] 342.38

 

 ABI 3130XL ID Genetica, DNA Laboratories, Inc., Cincinnati OH Inheritance (found in Father & Child)    
23.2 [9] 342.69, 342.67 ABI 3130 ID Pamela Larkin, St. Louis Metropolitan Police Department Crime Lab Re-amplified evidence sample 2
23.2 [10] 339.58 ABI 3500 ID Pamela Larkin, St. Louis Metropolitan Police Dept. Crime Lab Re-amplified evidence sample 1
23.2 [11] 341.96, 342.22 ABI 3130xl ID Plus Arlete Cercal and Juliana Paca, Secção de Biologia/ Laboratório Central de Criminalística, Angola Re-extracted and re-amplified with PrepFiler extraction method Blood and skin cells from the same case file First observation

23.3

342.37

ABI 310

ID

Cintia Alves, IPATIMUP, Porto, Portugal

Re-extracted and re-amplified

Portugese Caucasian sample

1 in 299
24.2 346.00 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville Observed in multiple samples    
24.2 [2] 345.57  345.72 ABI 3130 XL ID Laurent Cardin & Frederic G. Brard IRCGN - National Forensic Institute of the French Gendarmerie. Rosny-sous-Bois, France re-extraction and re-amplification FTA card - saliva 1/85000
24.2 [3] 343.86, 345.24 ABI 3130 ID Ranee Ho, St. Louis Metropolitan Police Department Crime Lab Re-amplified    
24.3424.05ABI3500QIAGEN QSAndrew Bishop, Montana State Crime Labre-amp1
25.1 252.20 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
25.2 349.19 - 349.33 ABI 3130 XL ID Laurent Cardin & Frederic G. Brard IRCGN - National Forensic Institute of the French Gendarmerie. Rosny-sous-Bois, France re-extraction and re-amplification FTA card - saliva 1 in 21000
25.3 350.09` ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville Re-amplified    
26.1350.49 - 350.70ABI 3500xlIdentifiler PlusZoharin Hassam, RCMP - National Forensic Servicesseen in multiple samplesvariant appeared in a reference buccal sample and 5 additional case profiles6
> 28 362.38 - 362.45 ABI 3130 XL ID Laurent Cardin & Frederic G. Brard IRCGN - National Forensic Institute of the French Gendarmerie. Rosny-sous-Bois, France re-extraction and re-amplification FTA card - saliva 8 in 85000
28.3 361.75, 361.59 ABI 3130 ID Laboratoire de sciences judiciaires et de médecine légale, Montréal, Canada Re-amplified With an allele 20.1 in vWA; suspect origin: Native population (Inuit) 2 times in the same case
28.3 (>28) [2] 362.35, 362.36 ABI 3130 ID Ashley Trausch, St. Louis Metropolitan Police Department Detected in evidence and reference standard Variant allele observed in two siblings from same case  
29 267.22 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
29.3 351.36 & 351.55 & 351.55

180.05

 ABI 3130 SGM+ 

 

MINIFILER

 

 

 

 Gregor Kljucevsek, Ministry of the Interior, Forensic Science Centre,Slovenia Re-extracted and re-amplified Observed in the oral reference of this individual and case sample 6 in 16000
>29 366.28 ABI 3130 ID DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-amplified and re-analyzed with ABI 3130   2 in 31,330
>29 [2] 367.82 ABI 3730 ID DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-analyzed with ABI 3730   1 in 31,330
>29 [3] 271.27 ABI 3130xl ESX 16 Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) Re-amplified and re-electrophoresed Reference sample 1 (first observation)
30 (>28)303.09ABI 3500xlPowerPlex Fusion 6CMonica Price, Washington State Patrol Crime LaboratoryRe- direct amplificationConvicted Offender
30.3 369.18 ABI 3130 ID Barbara Frankovick, Melody Josserand and Chris Larson, University of North Texas Center for Human Identification Re-amplified  and re-injected Re-amplified twice and re-injected three times for confirmation 1
33.1 221.7 ABI 3100 PS Nicole Hoffman     M U F S C     56

33 reported variants as of  12/30/16

 

Three Banded Allele Patterns

Allele Designation

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency
15, 17, 21ABI 3500XLPowerPlex Fusion 6CMonica Price, WA State Patrol Crime LabRe-injectionType 2, Convicted offender
16, 20, 21ABI 3500xlPowerPlex Fusion 6CMonica Price, WA State Patrol Crime LabRe-direct amplificationType 1, convicted offender
16, 22, 24 ABI 3130xl ID Plus Brian Simmons, Defense Forensic Science Center Re-amplified    
17, 18, 25 ABI 310 ID Johnson_E USACIL Re-extracted and Re-amplified    

 17, 19, 21

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500
17, 21, 22 ABI 3130xl ID Robert Dorion, WA State Patrol Crime Laboratory Re-extraction Convicted Offender  
17, 24, 25 ABI 3130XL ID Francis Hermitte & Frederic G. Brard, IRCGN - National Forensic Institute of the French Gendarmerie, Rosny-sous-Bois, France re-extraction and re-amplification FTA card - saliva

Type 2 tri-allelic pattern

 1 in 25000
17, 25, 26ABI 3500xl Fusion 6CMonica Price, WA State Patrol Crime LabRe-direct ampConvicted offender, Type 1
18, 19, 20ABI 3500PowerPlex Fusion 6CErica Mpoy, St. Louis Metropolitan Police Department Crime LabRe-extractedVariant was seen in the victim’s reference1
19, 20, 22 ABI 3500 ID Sarah Custis, St. Louis Metropolitan Police Department Re-extract, re-amplification    
19, 20, 24ABI 3500XLPowerPlex Fusion 6CMonica Price, WA State Patrol Crime Lab Re-direct amplificationConvicted offender, Type 1
20, 21, 22 ABI 3500 GlobalFiler Kelly Lawrence, Northeastern Illinois Regional Crime Laboratory Present in both sample and standard; standard was re-amplified   2
20, 22, 23 ABI 3130xl ID Robert Dorion, WA State Patrol Crime Laboratory Re-extracted Convicted Offender  
20, 24, 25ABI 3500PowerPlex Fusion 6CMolly Rider, Washington State Patrol Crime Laboratoryre-injected reference sample, also seen in evidence profileReference sample1
20, 25, 26 ABI 3130xl ID Plus Robin Guidry, Houston Forensic Science Center Re-injection, re-amplification

20: 327.85, 327.71, 327.98,

25: 347.34, 347.29, 347.36

26: 351.02, 350.99, 351.05

  
25, 27, 28 ABI 3130 ID Plus Kari O'Neill, WA State Patrol Crime Laboratory Re-extracted Type 1 tri-allelic pattern  
             

 10 reported tri-allelic patterns as of 12/30/16

 

* Use the following abbreviations for amplification kit names:

 

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