D19S433 Variants
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Allele Designation |
Allele Size |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
4 | 80.65 | ABI 3130xl | ID Plus | Zhang Huai-Cai, Wang Dong-Jing, Zhou Zhi-Quan, Hangzhou Institute of Criminal Science and Technology, China |
Re-amplify and sequencing Sequencing results of repeat region: AAGG AAAG AAGG TAGG [AAGG]2 AGGAGGTTGAGGCT |
The people related to the case | |
5 | 192.19 | ABI 3500 | PowerPlex Fusion 6C | Erik Hall, St. Louis Metropolitan Police Department Crime Lab | Re-amplification | 1 | |
5.2 |
87.36 |
ABI 3100 |
ID |
Sue |
Re-extract |
Convicted Offender |
1 |
5.2 [2] | 87.80 & 87.20 Avg |
ABI 310
& ABI 3100 |
ID | Lynette Stargardt, Independent Forensics. Hillside IL | Re-prepped , Re-amplified & Re-extracted | Shared between alleged siblings: not shared with tested parent | |
5.2 [3] | 87.34-87.46 | ABI 3130xl | ID | Catherine HOG, Laurent Cardin, Sylvain Hubac and Frederic Brard, National Forensic Science Institute of the French Gendarmerie. IRCGN, Rosny-sous-Bois, France | male reference sample on FTA (buccal cells) extracted and amplified twice separately | D19S433 heterozygous (5.2/15.2) | |
Approximately 5.3 (Slightly >5.2, but <5.3) | 194.71 & 194.44 | ABI 3500 | PowerPlex Fusion 6C | Ashley O’Leary, Saint Louis Metropolitan Police Department Laboratory | Re-amplified for confirmation | Variant allele did not appear in other extractions of the same DNA profile (evidentiary samples and reference sample). OL allele was only additional allele present in this sample (no indication of a mixture), but it did not appear to be normal artifact such as spectral overlap or stutter. | 1 |
6.1 | 196.00 | ABI 3500xl | PowerPlex Fusion Direct | Rhonda Williams, Oklahoma State Bureau of Investigation | Re-punched, re-amped | Convicted Offender | |
6.2 (<9) |
91.62 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
1 in 11500 |
6.2 |
91.57 |
ABI 3100 |
SGM+ |
Ayeleth Reshef, Mark Barash, IDFS< |
Re-amplified |
2 casework samples (3stains) and a duplicate of a reference sample |
1 |
6.2 [2] |
91.29
91.36 |
ABI 3130 XL | ID | Marie-Beatrice Tonanny & Frederic G. Brard IRCGN France | re-extraction and re-amplification | FTA card - saliva 6.2 homozygote | 3/30,000 |
6.2 [3] | 91.84 | ABI 310 | Katja Drobnic, Forensic Science Laboratory, Ministry of the Interior, Ljubljana. SLOVENIA | Re-extraction and re-amplification | Saliva sample of suspect - homozygote | 1 in 1700 | |
6.2 [4] |
89.5 (SEFiler) 116.8 (NGM SElect) |
ABI 3130 POP-4 | SEFiler & NGM SElect | Peter Schneider, Institute of Legal Medicine, University of Cologne, Germany | inheritance from mother to child confirmed with two amplification kits | Family from Middle East Asia | N/A |
6.2 [5] | 91.40 | ABI 3130 | ID | Shannon Soltysiak, NYC OCME | buccal swab cut twice for extraction, confirmed in 3130 run of each amp | 2 (both 3130 runs) | |
6.3 | 120.47 | ABI 3500 | Globalfiler | HOLLARD Clémence – VILLET Noémie – CHERPIN Marie-Hélène, Service National de Police Scientifique – Laboratoire de Police Scientifique de Lyon | Re-amplified & re-analyzed with NGS (Illumina) | 2x (shaver & muscle) | |
7 |
94 bp |
ABI 310 |
ID |
Andre de Kock |
Re-amplified |
|
37 in 729 |
7 [2] |
93.47 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amplification |
Alabama Department of Forensic Science Convicted Offender |
2 in 8844 |
7 [3] | 93.3 | ABI 3100 | ID |
Angela Withrow M U F S C |
|||
7 [4] | 93.7 | ABI 3130XL | ID | Jennifer Montgomery, MA State Police Forensic and Technology Center | Re-run to confirm | Convicted Offender | 1 |
7 [5] | 93.91 & 94.00 | ABI 310 | ID | Daniel Gregonis, Los Angeles Sheriff's Department | 8 separate but case related samples including sperm and non-sperm DNA extracts | multiple items in same case | 1 |
7 (<9) | 93.51 | ABI 3100 | ID |
Angela Withrow M U F S C |
|||
7 (<9) [2] | 93.73 | ABI 3130 | ID | Erica Oppelz, St. Louis Metropolitan Police Department Crime Lab | Reference sample | 1 | |
7.2 | 95.12 | ABI 3500 | ID and NGM | Eduardo Leal Rodrigues, Laboratório Centro de Genomas | Re-extracted and re-amplified by using two different amplification kits | ||
7.2 [2] | 201.02 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Crime scene sample | 1 (first observation) |
8 |
97.44 |
ABI 3100 |
ID |
Angela Zimmerman MU F S C |
Re-amplification |
Alabama Department of Forensic Science Offender |
1 in 5367 |
8 [2] | 97.39 | ABI 3130 | ID Direct | DASA - Nelson Gaburo Junior - Department of Molecular Biology - Diagnosticos da America - DASA | Re-amplified and Re-analyzed | ||
8.2 | 100 | ABI 310 | ID | Cindy Carroll Los Angeles County Sheriff's Department | Re-extracted and re-amplified | Buccal reference | 1 |
8.2 [2] | 99.79 | ABI 3130xl | ID | Genetica DNA Laboratories, Cincinnati OH | Inheritance (child and father) | ||
<9 |
97.61 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amplification |
Alabama Department of Forensic Science Offender |
6 in 13652 |
<9 [2] | 97.8 | ABI 3100 | ID | Angela Withrow M U F S C | |||
<9 [3] | 93.57 | ABI 3100 | ID | Angela Withrow M U F S C | |||
<9 (7) [4] | 93.3 | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3730 | 1 in 31,330 | |
9.1 | 102.5 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-extracted | ||
9.2 | 106.63 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-extracted | ||
10.1 | 106.42 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-extracted | ||
10.1 [2] | 106.59 | ABI 3130 | ID | Karen Preiter, St. Louis Metropolitan Police Dept | Extracted multiple evidence samples with same off-ladder allele | 1 in 18159 | |
10.1 [3] | 106.7 | ABI 3130 and ABI 3500xl | ID | Erica Oppelz, St. Louis Metropolitan Police Department Crime Lab | Re-amplified | Variant was seen in the victim's reference as well as evidence samples | |
10.1 [4] | 212.15 | ABI 3500XL | Fusion 6C | Matthew Fisher, Washington State Patrol Crime Lab | Re-injection | ||
10.2 | 107.31 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-amplified | ||
10.2 [2] | 107.32 & 107.33 | ABI 3130 | ID | Brittany Ross, KY State Police Forensic Lab, Frankfort KY | Reanalysis | Offender Sample | |
10.2 [3] | 213.13 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-amplified & re-analyzed | immigration case (CH only) | 1 in 2730 |
11.1 |
112.29 |
ABI 3100 |
SGM+ |
Ronny Decorte, Laboratory for Forensic Genetics and Molecular
Archaeology, |
Re-extracted and Re-amplified |
Paternity sample, Caucasian |
1 in 3155 (mainly Caucasians |
11.1 [2] |
110.43 |
ABI 3100 |
ID |
Angela Withrow Ml U F S C |
Re-amp x 1 |
|
1 in 15543 |
11.1 [3] | 215.73 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
11.2 | 111.42 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville | Re-amplified | ||
11.2 [2] | 111.47 | ABI 3100 | SGM+ | Mark Barash, IDFS, DNA lab, Israel | 2 casework samples, re-amplified | 1 | |
11.2 [3] | 135.83 | ABI 3500 | NGM SElect | Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country | Re-amplified and re-analyzed | Convicted offender sample | |
11.2 [4] | 136.02 | ABI 3500 | NGM SElect | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender sample | |
11.2 [5] | 217.14 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-amplified & re-analyzed | Immigration case (AM+CH) | 2 in 2730 |
11.2 [6] | 217.12 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-amplified & re-analyzed | Immigration case (AF+CH1+CH2) | 5 in 2730 |
11.2 [7] | 243.22 | ABI 3500 | QIAGEN Investigator 24plex QS kit | Andrew Bishop, Montana State Crime Lab | Re-amplification | Evidence sample | 1 |
11.2 [8] | 242.80, 242.87, 242.86 | ABI 3500xl | Investigator IDplex Plus Kit | Komal Mahajan - Regional Forensic Science Laboratory, Pune, India. | Evidence sample and Reference sample | ||
12.1 |
114.58 114.61 |
ABI 310 |
ID |
Kelly Beatty M U F S C |
Re-extraction |
US Embassy, Phillipines - Immigration |
5 in 15410 |
12.1 [2] |
114.36 +/-0.09 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
4 in 11500 |
12.1 [3] |
114.54 |
ABI 310 |
ID |
Johnson_E USACIL |
Re-extracted and Re-analyzed |
Reference Blood |
|
12.1 [4] |
114.55 |
ABI 310 |
ID |
Overson_T USACIL |
Re-extracted and Re-analyzed |
Reference Blood |
|
12.1 [5] |
34 |
ABI 310 |
ID |
Johnson_E USACIL |
Re-extracted and Re-analyzed |
Reference Blood |
|
12.1 [6] | 114.2 | ABI 3100 | ID | Angela Withrow M U F S C | |||
12.1 [7] | 114.18 | ABI 3100 | ID | Angela Withrow M U F S C | |||
12.1 [8] | 114.22 | ABI 3100 | ID | Angela Withrow M U F S C | |||
12.1 [9] | 114.23 | ABI 3130xl | ID Plus | Pinellas County Forensic Laboratory | Reinjection | 1 | |
12.1 [10] | 113.55 | ABI 3500 | ID | Pamela Larkin, St. Louis Metropolitan Police Department Crime Lab | Re-amplified | evidence sample | 1 |
12.1 [11] | 3500: 113.55, 113.56, 113.50; 31300: 114.23 | ABI 3500 & 3130 | ID | Pamela Larkin, St. Louis Metropolitan Police Department Crime Lab | Re-amplified | evidence sample and reference sample | 4 |
12.1 [12] | 219.72 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Crime scene sample | 1 (first observation) |
12.1 [13] | 113.63 | ABI 3500 | ID | Pamela Larkin, St. Louis Metropolitan Police Department Crime Lab | re-amplified | evidence sample | 1 |
12.2 | 115.08 | ABI 3730 | Applied Biosystems Identifiler Plus | Aqsa Rubab, Muhammad Shafique, Ahmad Ali Shahid, Forensic DNA Typing Lab, CEMB, University of the Punjab Lahore, Pakistan. | Re-amplified and Reanalyzed. | Christian population samples | 13 in 506 |
13.1 |
118.34 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
1 in 11500 |
13.1 [2] | 142.66 | ABI 3500 | NGM SElect | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender sample | |
13.1 [3] | 224.08 | ABI 3500xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
13.2 |
|
ABI 377 |
Made in Lab |
Mechthild Prinz Office of Chief Medical Examiner
|
Sequenced |
|
|
13.3 |
120.25 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amplification |
Alabama Department of Forensic Science Convicted Offender |
1 in 14245 |
14.1 |
122.24 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
1 in 11500 |
14.1 [2] | 122.17 | ABI 3130XL | ID | Genetica, DNA Laboratories, Inc., Cincinnati OH | Inheritance (found in Child and Father) | ||
14.1 [3] | 227.63 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
14.2 | 123.05 | ABI 3730 | Applied Biosystems Identifiler Plus | Aqsa Rubab, Muhammad Shafique, Ahmad Ali Shahid, Forensic DNA Typing Lab, CEMB, University of the Punjab Lahore, Pakistan. | Re-amplified and Reanalyzed. | Christian population samples | 89 in 506 |
14.3 |
124.15 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
1 in 11500 |
14.3 [2] | 124.42 | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3730 | 5 in 31,330 | |
15.2 | 128.03 | ABI 3730 | Applied Biosystems Identifiler Plus | Aqsa Rubab, Muhammad Shafique, Ahmad Ali Shahid, Forensic DNA Typing Lab, CEMB, University of the Punjab Lahore, Pakistan. | Re-amplified and Reanalyzed. | Christian population samples | 78 in 506 |
15.3 | 127.09 | ABI 3130xl | ID | Tara Roy, Washington State Patrol Crime Laboratory | Re-extraction | Convicted Offender | |
16.1 | 130.47 | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3730 | 1 in 31,330 | |
16.1 [2] | 130.28 | ABI 3130 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3130 | 1 in 31,330 | |
16.2 | 131.13 | ABI 3730 | Applied Biosystems Identifiler Plus | Aqsa Rubab, Muhammad Shafique, Ahmad Ali Shahid, Forensic DNA Typing Lab, CEMB, University of the Punjab Lahore, Pakistan. | Re-amplified and Reanalyzed. | Christian population samples | 13 in 506 |
17.1 | 239.88 | ABI 3500 | PP21 | François Chatenay-Lapointe, Rhode Island Blood Center | Re-amplified and Re-analyzed | Immigration case. | 2 in 3772 |
17.2 | 163 | ABI 3130 | Applied Biosystems Huaxia Platinum | Yingai Zhang, Shunlan Wang, Central laboratory, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, China. | Re-extracted, re-amplified and re-analyzed | 1 | |
>17.2 | 136.46 | ABI 3130xl | ID | Sherri Phillips, Washington State Patrol CODIS Laboratory | Re-extracted | ||
18 |
137 |
ABI 310 |
SGM |
A.Jonkisz,M.Zoledziewska, A.Lebioda,E.Kowalczyk, T.Dobosz |
Re-amplified |
Polish Lower Silesian Population |
1 in 600 |
18 [2] |
137.15 |
ABI 3100 |
ID |
Faruk Asicioglu |
Re-extracted and re-amplified |
Maternal heritage, mother & son |
2 |
18 [3] |
137.23 |
ABI 3100 |
ID |
Laimis Stankevicius, Lithuanian Police Forensic Science centre |
Re-amplified |
Lithuanian Police, Forensic science centre, paternity father and son |
1 in 1350 |
18 [4] | 137.27 | ABI 310 | ID | Demeter József Szabolcs, Popescu Octavian, Molecular Biology Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes Bolyai University | Re-extracted and re-amplified | 1 of 424 | |
18.1 | 138.33 138.47 | ABI 3100 Avant | ID | Julie M. Marschner, Las Vegas Metropolitan Police Department Forensic Laboratory | Re-amplification of reference standard | 2 casework samples with matching reference standard of suspect | 1 (three times in one case) |
18.1 [2] | 138.68, 138.54 (SGM+); 164.00, 163.92 (NGM); 138.22, 138.23 (ID+) | ABI 3130xl |
SGM+ NGM ID+ |
Monnie Michalik, North Louisiana Criminalistics Laboratory | Re-amplified and re-amplified using different kits | 2 samples from same person | |
18.1 [3] | 166.37 | ABI 3500 | Globalfiler | Ian Perry; West Virginia State Police Forensic Lab, Biology Unit: Databasing Section | re-extraction and reamplification | Offender Sample | 1 |
18.2 (>17.2) |
139.59 +/-.0.1 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
2 in 11500 |
18.2 |
135.51 |
ABI 3100 |
ID |
Timothy D. Kupferschmid
Myriad Genetic Labs,
|
Reamplified |
|
|
18.2 [2] |
139.42, 139.25 |
ABI 3100-Avant |
ID |
Forensic DNA Institute at
|
|
These 2 specimens are from an alleged father and his son in a paternity case |
1 in 170 |
18.2 [3] | 139.44 | ABI 3130 xl | ID | Marie-Beatrice Tonanny & Frederic G. Brard, IRCGN, National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois, France | Re-extraction and re-amplification | FTA card - saliva. | 23/154.000 |
18.2 [4] | 139.31 | ABI 310 | ID | Demeter József Szabolcs, Popescu Octavian, Molecular Biology Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes Bolyai University | Re-extracted and re-amplified | 3 of 424 | |
18.2 [5] | 139.36 | ABI 310 | ID | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender sample | |
18.2 [6] | 139.44 | ABI 310 | ID | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Criminal case sample, no suspect | |
18.2 [7] | 163.41 | ABI 3500 | NGM SElect | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Criminal case sample, no suspect | |
18.2 [8] | 163.54 | ABI 3500 | NGM SElect | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender sample | |
18.2 [9] | 163.56 | ABI 3500 | NGM SElect | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender sample | |
18.2 [10] | 163.46 | ABI 3500 | NGM SElect | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender sample | |
19 (>17.2) |
141.76 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
1 in 11500 |
19 [2] | 141.66 | ABI 3130 | ID | Laboratoire de sciences judiciaires et de médecine légale, Montréal, Canada | Re-analyzed, re-amplified | ||
19.2 |
143.71 |
ABI 3100 |
ID |
Daiva Kairiene, Lithuanian Police Forensic Science Centre |
Re-extracted and Re-amplified |
Lithuanian Police Forensic science centre, comparative blood sample and blood stain |
1 in 1300 |
19.2 [2] | 144.15 | ABI 3100 | SGM plus | Shlomit Avraham, Israel Police, Jerusalem, Israel | Saliva on FTA Card, 3 Funs from same PCR | 1 | |
19.2 [3] | 143.85 & 143.73 | ABI 3130XL | ID | Regina Robinson, KY State Police Forensic Lab, Frankfort KY | Re-analysis | ||
19.2 [4] | 143.74 | ABI 310 | ID | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Re-amplified and re-analyzed | Convicted offender sample | |
19.2 [5] | 143.76 | ABI 3500 | ID and NGM | Eduardo Leal Rodrigues, Laboratório Centro de Genomas | Re-extracted and re-amplified by using two different amplification kits | ||
19.2 [6] | 143.71 | ABI 3130 | ID Direct | Nelson Gaburo Junior - Department of Molecular Biology - Diagnosticos da America - DASA | Re-amplified and Re-analyzed | ||
19.2 [7] | 143.95(1), 143.96(2), 143.92(3) | ABI 3130 | ID | Amy Rathmann, St. Louis Metropolitan Police Department Crime Lab | Re-amplified and detected in multiple samples in same case | ||
20 |
145 |
ABI 310 |
ID SGM |
A.Jonkisz,M.Zoledziewska, A.Lebioda,E.Kowalczyk, T.Dobosz |
Re-amplified |
Paternity testing trio |
2 in 600 |
20 [2] | 146.06 | ABI 3130XL | ID | Jennifer Montgomery, MA State Police Forensic and Technology Center | Re-run to confirm | Convicted Offender | 1 |
20 (>17.2) [3] | 145.95 | ABI 3100 | ID | Kelli Byrd, Orchid Cellmark, Dallas, TX | Re-amplified both samples with ID and re-amplified both samples with PS to confirm correct locus | present in 2 casework samples from same case | 2 |
20.1 | 146.02 | ABI 3100 | ID | Anglia DNA Services Limited, UK | Re-amplification | 1 | |
20.2 (>17.2) | 148.23 | ABI 3130xl | ID | Sherri Phillips, Washington State Patrol CODIS Crime Lab | Re-extracted | ||
20.2 [2] | 146.61 | ABI 3500 | ID | Amy Rathmann, St. Louis Metropolitan Police Department Crime Lab | Detected in multiple samples in the same case | ||
20.2 [3] | 175.06 | ABI 3500 | GlobalFiler | Ashley Callanan & Shanna Saunders, St. Louis County Police Crime Laboratory | Re-injection at 10s, 15s, 20s | Possible blood sample, single-source | 1 |
20.2 [4] | 254.31, 254.07 | ABI 3500 | PowerPlex Fusion 6C | Krista Spear, St. Louis Metropolitan Police Department Crime Laboratory | Re-amplified | Reference standard | 1 |
20.2 [5] | 254.28 | ABI 3500 | PowerPlex Fusion 6C | Erica Mpoy, St. Louis Metropolitan Police Department Crime Lab | Reamplified | Variant was seen in an evidence sample | 1 |
>20.2 | 254.50 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Crime scene sample | 1 (first observation) |
21.2 | 152.51 (ID/3130xl), 257.47 (Fusion/3500xl) | ABI 3130xl, ABI 3500xl | ID, PowerPlex Fusion (5C) | Brittany Ross and Regina Wells, Kentucky State Police Forensic Lab | Sample was amplified twice in Identifiler and once in PowerPlex Fusion | In Identifiler, the variant peak called as <11 (~10.2) in vWA, but appeared to be overlap from D19S433 as a > 17.2 (~21.2). When retyped in PowerPlex Fusion the peak typed as >17.2 in D19S433 | |
21.2 [2] | 149.60, 149.59 (SiFaSTR 23 plex kit), 179.05, 178.97 (Huaxia Platinum PCR Amplification kit) | ABI 3130xl, ABI 3500xl | SiFaSTR 23 plex kit (Academy of Forensic Science, China) Huaxia Platinum PCR Amplification kit (Applied Biosystem) | Shanghai Key Laboratory of Forensic Medicine, Academy of Forensic Science, Shanghai, China | Re-amplify and sequencing, Sequencing results of repeat region: AAGG AA-- AAGG TAGG [AAGG]20 | This peak was detected between D19S433 and D16S539 when it amplified with SiFaSTR 23 plex kit. By using Huaxia Platinum PCR Amplification kit, GeneMapper ID-X detected a tri-allelic pattern in TH01 locus. After comparing the data from two kits and sanger sequencing, we confirmed this peak is actually a new allele of the D19S433 locus. This allele is shared between a child and father in a paternity case. | 2 |
26.3 | 198.79 | ABI 3130XL | Applied Biosystems VeriFiler Express | Jennifer Malcolm and Melanie Marty, Genomic Diagnostics, Melbourne Australia | Re-amplified, single-plex and Next Generation Sequencing | This peak was not observed with Identifiler but was detected when amplified with VeriFiler Express and NGM Detect. This peak overlaps into the TH01 region in VeriFiler Express and the D22S1045 region with NGM Detect giving the appearance of a tri-allelic pattern, but this peak is actually a variant of the D19S433 marker. This peak is shared between a child and father. | 2 |
27 | 173.80 | ABI 3130 | SGM Plus | Janine Stickley, Orchid Cellmark UK | Re-amplified with SGM Plus and confirmed designation with ID (173.76bp) | 1 |
38 reported variants as of 01/31/2017
Tri-Allelic Patterns
Allele Designation |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
6, 13, 14 | ABI 3500 | PowerPlex Fusion 6C | William Christensen, OCPD Laboratory Services Division (DNA/Serology) | In two question samples, both sample re-amplified | Questioned samples from blood; Allele Sizes: 6 (195.92); 13 (224.07); 14 (228.09) | |
12, 13, 14 | ABI 3130xl | ID |
Monica Price, WA State Patrol Crime Lab |
Re-extraction |
Convicted offender |
|
12, 13, 14 [2] | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-amplified and re-analyzed with ABI 3730 | 1 in 31,330 | |
12, 14, 15 | ABI 3130XL | ID | Sylvain Hubac & Frederic G. Brard,IRCGN, National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois, France | Re-extraction and re-amplification | FTA card - saliva. -type 2 allelic pattern | 1/160,000 |
12, 14, 15 [2] | ABI 3500XL | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Convicted Offender, Type 1 | |
12, 15.2, 16.2 | ABI 3130 | ID | Brenda Crosby, St. Louis Metropolitan Police Department Crime Lab | Re-extracted | ||
12.2, 13.2, 14 | ABI 3130xl | ID | Robert Dorion, WA State Patrol Crime Laboratory | Re-extraction | Convicted offender | |
13, 14, 15 | ABI 3130xl | ID | Robert Dorion, WA State Patrol Crime Laboratory | Re-extraction | Convicted offender | |
13, 14, 16 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
1 in 11500 |
13, 15, 16 | ABI 3130xl | ID | Francis Hermitte & Frederic Brard, National Forensic Science Institute of the French Gendarmerie, IRCGN, Rosny-sous-Bois, France | male reference sample on FTA (buccal cells) extracted and amplified twice separately |
type 1 pattern 1st analysis: 13=1163rfu, 15=289rfu, 16=879rfu 2nd analysis: 13=899 rfu, 15=256rfu, 16=651rfu |
1 in 70,000 |
14, 14.2, 15 | ABI 3130 | ID Plus | Marion Clark, WA State Patrol Crime Laboratory | Re-extraction |
Buccal swab Allele sizes: 120.22, 122.22, 124.17 |
|
14, 14.2, 15.2 | ABI 3130 XL | ID | Sylvain Hubac & Frederic G. Brard,IRCGN, National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois, France | Re-extraction and re-amplification | FTA card - saliva - type 1 tri-allelic pattern | 1 in 131,650 |
14, 15, 16 | ABI 3500 | ESI 16 |
Horolma Pamjav, Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary |
Observed in Hungarian population data | 1 in 21,473 | |
14, 15, 16 [2] | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-direct amplification | Convicted offender, Type 1 | |
14, 15.2, 16.2 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police
|
Re-amplified and re-analyzed |
|
1 in 11500 |
14, 16.2, 17.2 | ABI 3130 | Identifiler Plus | Beau Baggenstoss, WA State Patrol Crime Laboratory | Re-extraction and re-amplification | Allele Sizes: 119.81, 129.79, 133.65 | 2 |
14, 16.2, 17.2 [2] | ABI 3130xl | ID | Robert Dorion, WA State Patrol Crime Laboratory | Re-amplified | Convicted offender |
12 reported tri-allelic patterns as of 01/31/2017
* Use the following abbreviations for amplification kit names:
- CO: Applied Biosystems COfiler
- PR: Applied Biosystems Profiler
- PS: Applied Biosystems Profiler Plus
- ID: Applied Biosystems Identifiler
- PP: Promega PowerPlex 16
- CV: Promega CTTV
- CA: Promega CTTA
- MP: Promega MonoPlex
- Other: Please specify