D16S539 Variants
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|
Allele Designation |
Allele Size |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
|
4 |
257.74 |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 69600 |
|
6 |
237.30 |
ABI 377 |
CO |
Re-extracted and reamplified |
|
|
|
|
6 [2] |
233.11 |
ABI 310 |
PS,CO |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 32660 |
|
6 [3] |
233.44 |
ABI 310 |
CO |
Jan Nicklas,VT Forensic Laboratory |
Re-amp |
|
|
|
6 [4] |
264.8 |
ABI 3130 |
PP 16 |
Jason Hodges, WV State Police Crime Lab |
Re-amplified |
Convicted Offender |
|
|
6 [5] |
256.03 |
ABI 3100 |
ID |
Marsha Garcia, Orchid Cellmark, Nashville |
Re-amplified |
|
|
|
7 |
237.50 |
ABI 310 |
CO |
Russell Vossbrink |
Observed in 1 k and 3 q's |
Casework |
|
|
7 [2] |
269.98 |
ABI 3100 |
PP |
Cory Formea, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 |
|
7 [3] |
260.10 |
ABI 3100 |
ID |
Marsha Garcia, Orchid Cellmark, Nashville |
Re-amplified |
|
|
| 7 [4] | 261 | ABI 310 | ID |
Jiri Drabek, Czech forensic genetics expert, Olomouc |
pedigree (mother 7,12 and child 7,12) |
Paternity case. Frequency (number on times observed): twice (mother and child); the largest set of the published Czech population frequencies (Simkova et al., 2009) had number of alleles=2822 for D16S539 without allele 7 encountered, so p=1/(2822+1)=0.000354233. |
|
|
8.3 |
267.94 |
ABI 310 |
ID |
Angelo Abati, Institut de medecine legale de LIEGE |
3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele |
|
1 in 800 convicted offenders (+ the father) |
|
8.3 [2] |
277.45 |
ABI 310 |
PP16 |
Angelo Abati, Institut de medecine legale de LIEGE |
3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele |
|
1 in 800 convicted offenders (+ the father) |
|
9.3 |
248.29 |
ABI 310 |
CO |
MO State Highway Patrol |
Re-extracted and re-amplified |
Convicted Offender |
1 in 4514 to date |
|
9.3 [2] |
271.71 |
ABI 310 |
ID |
Angelo ABATI, |
3 times in ID |
|
1 in 1100 |
|
9.3 [3] |
281.26 |
ABI 310 |
PP 16 |
Angelo ABATI, |
3 times in PP16 |
|
1 in 1100 |
|
9.3 [4] |
271.37 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police |
Re-amplified and re-analyzed |
|
1 in 11500 |
|
9.3 [5] |
248.02 |
ABI 3100 |
PS |
Angela Withrow M U F S C |
|
|
|
| 9.3 [6] | 271.52 | ABI 310 | ID | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Criminal case sample | |
|
10.1 |
283.01 |
ABI 3130XL |
PP |
Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory |
Confirmed with multiple samples |
|
|
| 10.1 [2] | 294.54 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
| 10.1 [3] | 272.40, 272.35, 272.41, 272.42 | ABI 3500XL | Identifiler PCR Amplification Kit | Komal Mahajan - Regional Forensic Science Laboratory, Pune, India. | Re-amplification. | Evidence sample and Reference sample (Both samples repeated twice) | |
| 10.2 | 273.18 | ABI 3130 | ID | Amy Jagmin, Washington State Patrol Crime Laboratory | present in two different samples, re-injected | samples were mixed and variant allele in each was a minor contributor | 2 |
|
10.3 |
275.07 |
ABI 3100 |
ID |
Marsh Garcia, Orchid Cellmark, Nashville TN |
Re-injected |
|
|
|
10.3 [2] |
275.07 |
ABI 3100 |
ID |
Marsh Garcia, Orchid Cellmark, Nashville TN |
Observed in multiple samples, re-injected |
|
|
|
10.3 [3] |
274.9 |
ABI 3100 |
ID |
Marsh Garcia, Orchid Cellmark, Nashville TN |
Observed in multiple samples, re-injected |
|
|
| 10.3 [4] | 278.16 | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3730 | 2 in 31,330 | |
| 10.3 [5] | 296.10 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
|
11.1 |
287.01 |
ABI 3100 |
PP |
Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory |
Re-Extraction |
|
|
| 11.1 [2] | 276.46 | ABI 3500xl | ID | Anglia DNA Services, UK | Re-injection | 1 | |
|
11.3 |
256.27 |
ABI 310 |
CO |
David Young |
Multiple amplifications |
Evidence samples |
1 in 7500 to date |
|
11.3 [2] |
279.13 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amplified |
|
3 in 12115 |
|
11.3 [3] |
288.85 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 32660 |
|
11.3 [4] |
279.25 |
ABI 3100 |
ID |
Paul Mazur |
Multiple amplifications |
Casework sample |
|
|
11.3 [5] |
278.96 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
|
|
|
|
11.3 [6] |
278.96 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
|
|
|
| 11.3 [7] | 279.22 | ABI 3130 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3130 | 2 in 31,330 | |
| 11.3 [8] | 280.04 | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3730 | 1 in 31,330 | |
| 11.3 [9] | 300.69 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Crime scene sample | 1 (first observation) |
|
12.1 |
258.39 |
ABI 310 |
CO |
Re-extracted and re-amplified |
|
|
|
|
12.1 [2] |
293.04 |
ABI 310 |
PP 1.2 |
Greg Sykes ATCC |
Re-extracted and Re-amplified |
From a colon carcinoma with integrated CMV-promoter-driven GADD45a expression vector, ATCC cell line CRL-2579; name: RKO-AS45-1 |
|
|
12.1 [3] |
290.85 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 32660 |
|
12.1 [4] |
290.98 |
ABI 3100 |
PP |
ICMP |
Re-extraction and amplification or observed intergenerational inheritance |
Blood reference |
2 in 11000 |
|
12.1 [5] |
281.42 |
ABI 3100 |
ID |
Marsha Garcia, Orchid Cellmark, Nashville |
|
|
|
| 12.1 [6] | 281.65 | ABI 3130xl | ID Direct | Nelson Gaburo Junior - Department of Molecular Biology - Diagnosticos da America - DASA | Re-amplified and Re-analyzed | ||
| 12.1 [7] | 302.31 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Crime scene sample | 1 (first observation) |
|
12.2 |
263.17 |
ABI 377 |
CO |
Kelly Solis |
Re-amplified |
Convicted offender |
1 in 35000 |
|
12.3 |
283.46 |
ABI 3100 |
ID |
Marsha Garcia, Orchid Cellmark, Nashville |
Re-amplified |
|
|
| 12.3 [2] | 304.33 | ABI 3130xl | ESX 16 | Malin Sanga, Swedish National Laboratory of Forensic Science (SKL) | Re-amplified and re-electrophoresed | Reference sample | 1 (first observation) |
|
13.1 |
297.18 (POP-4) 299.84 (POP-6) |
ABI 310 |
PP |
Greg Sykes ATCC |
Re-amplified three times and run using high resolution POP-6 polymer |
allele discovered in a colon carcinoma cell line transfected with HPV E6 inserted in pCMV.3 (name designation: RKO-E6) |
|
|
13.2 |
286.15 |
ABI 3100 |
ID |
Marsha Garcia, Orchid Cellmark, Nashville
|
Re-injected |
|
|
| 13.2 [2] | 286.62; 286.57 | ABI 3130xl | ID | Christine Barnes, St. Louis Metropolitan Police Dept, St. Louis, MO | Re-amplified | OL observed in multiple evidence samples | |
|
13.3 |
267.93 |
ABI 377 |
CO, PS |
Re-extracted and re-amplified |
|
|
|
|
13.3 [2] |
287.18 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amplified |
|
2 in 12,812 |
|
13.3 [3] |
296.97 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 33739 |
|
13.3 [4] |
287.65 +/-0.18 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police |
Re-amplified and re-analyzed |
|
4 in 11500 |
|
13.3 [5] |
264.47 |
ABI 3100 |
CO |
Angela Withrow M U F S C |
Previously seen - not verified |
|
3 |
|
13.3 [6] |
264.34 |
ABI 3100 |
CO |
Nicole Hahn M U F S C |
Previously Seen – no verification |
|
4 |
|
13.3 [7] |
264.47 |
ABI 3100 |
CO |
Nicole Hahn M U F S C |
Previously Seen – no verification |
|
5 |
| 13.3 [8] | 286.53 | ABI 3500 | Identifiler Plus | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender | |
| 13.3 [9] | 286.47 | ABI 3500 | ID Plus | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender | |
| 13.3 [10] | 287.02 (1), 287.04 (2), 287.03 (3) | ABI 3130 | ID | Brenda Crosby, St. Louis Metropolitan Police Department Crime Lab | Re-amplified and detected in multiple intimate samples from the same individual | ||
|
14.1 |
308.45 |
ABI 3100 |
PPX16 |
Marsha Garcia, Orchid Cellmark, Nashville TN |
Re-amplified |
|
|
|
14.2 |
290.27 |
ABI 3100 |
ID |
Marsha Garcia, Orchid Cellmark, Nashville TN |
re-injected |
|
|
|
14.3 |
272.02 |
ABI 337 |
CO |
Nicole Swinton |
Re-extracted and re-amplified |
|
|
|
16 |
273.57 |
ABI 310 |
CO |
Carolyn Booker |
Reproduced in blood, saliva, hair, and fingernail samples |
Discovered during validation testing |
One individual |
|
16 [2] |
273.81±0.10 |
ABI 3100 |
CO |
Jan Nicklas,VT Forensic Laboratory |
Re-amp |
|
2 in 3750 |
|
16 [3] |
306.10 |
ABI 3100 |
PP |
ICMP |
Re-extraction and amplification or observed intergenerational inheritance |
Blood reference |
1 in 11000 |
|
16 [4] |
296.29 |
ABI 3100 |
ID |
Marsha Garcia, Orchid Cellmark, Nashville |
Re-extracted |
|
|
|
16 [5] |
273.56 (average of two injections) |
ABI 310 |
CO |
Andrew Garinger, Illinois State Police |
|
Observed in both victim standard and sample F1. |
|
| 16 [6] | 127.55 | ABI 3500 | PP21 | Massimo Mangiola, Rhode Island Blood Center | Re-amplified & re-analyzed | Immigration case (AM+CH) | 2:1128 |
|
16 (>15) |
305.96 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 32660 |
| 17 | 277 | ABI 3130 | SGM+ | Rivka Adelman, Israeli Police | Observed in 4 PCR of 3 items and 1 Reference (the same allele size) | Casework |
22 reported variants as of 08/04/2016
Tri-Allelic Patterns
|
Allele Designation |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
|
7, 11, 12 |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
| 9, 9.2, 11 | ABI 3130 | PP16HS | Compton Beecher, Caribbean Genetics Kingston 7 Jamica | Duplicate | 9.2 Allele half peak heights of other alleles | 2 |
| 9, 10, 11 | ABI 3130xl | ID | Robert Dorion, Washington State Patrol Crime Laboratory | Re-extraction | Convicted Offender | |
| 9, 10, 12 | ABI 310 | ID | Kelly Pokorny & Kyra Groeblinghoff, St. Louis County Police Crime Laboratory | Reinjection, re-amplification and re-extraction | Allele 10 repeatedly amplifies at much lower RFU than 9,12 alleles | 1 |
| 9, 10, 12 [2] | ABI 3500 | ESI 16 | Horolma Pamjav, Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary | Observed in Hungarian population data | 1 in 21,473 | |
|
9, 11, 12 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amp x 1 |
|
1 in 15144 |
| 9, 11, 12 [2] | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-amplified and re-analyzed with ABI 3730 | 1 in 31,330 | |
| 9, 11, 12 [3] | ABI 3130 | ID | Pamela Larkin, St. Louis Metropolitan Police Department Crime Lab | Re-amplified | Reference sample | 1 |
| 9, 11, 12 [4] | ABI 3500 | GlobalFiler | Josh Haynes, West Virginia State Police Forensic Laboratory | Allele Size: 243.92, 252.04, 256.02 | ||
| 9, 13, 14 | ABI 3130xl | ID | Robert Dorion, Washington State Patrol Crime Lab | Re-extraction | Convicted offender | |
| 9.2, 11, 12 | ABI 310 | PP16HS | Ashley Hayes, Ann Arundel Co. Police Dept. Crime Lab, MD | Re-extracted | 1 | |
| 10, 11, 12 | ABI 3500XL | Global Filer Express/Power Plex Fusion-5c | Central Laboratory for the National DNA Database, Ministry of Justice, Department of Penitentiary Administration, Penitentiary Police- Rome Italy | Allele Size: 98.30, 102.68, 107.12 | ||
|
10, 12, 13 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police |
Re-amplified and re-analyzed |
|
1 in 11500 |
| 10,12,13 [2] | ABI 3500 | GlobalFiler | Tara Scott, Iowa DCI Crime Lab | Re-amplified | Allele Size: 248.15/256.08/260.00 | Once |
|
10, 12, 14.2 |
ABI 3130xl |
|
Robert Dorion, Washington State Patrol Crime Laboratory |
|
|
|
| 10, 14, 15 | ABI 3500 | GlobalFiler | Cristian Hernandez, Costa Rica Forensic Science Department | re-amplification | crime scene sample - Allele Size: 248.04, 264.06, 268.14 | 1 |
|
11, 12, 13 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amp x 1 |
|
1 in 14767 |
|
11, 12, 13 [2] |
ABI 310 |
PP 16 |
Anthony J. Tambasco, Mansfield, Ohio, Police Lab |
Re-injection |
Two seperate crime scenes and suspect standard |
1 |
| 11, 12, 13 [3] | ABI 310 | ID Plus | Winnie Kurowski, Acadiana Criminalistics Lab, New Iberia, LA | Re-amplified, re-injected | Reference sample | N/A |
|
11, 13, 14 |
ABI 3100 |
ID |
Angela Withrow M U FS C |
Two extractions/Three amps |
|
1 in 12812 |
|
11, 13, 14 [2] |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
| 11, 13, 14 [3] | ABI 3500 | ESI 16 |
Horolma Pamjav, Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary |
Observed in Hungarian population data | 1 in 21,473 | |
|
12, 13, 14 |
ABI 377 |
CO |
Karl Morton
|
Re-extracted |
Convicted offender |
1 in 39,000 |
|
12, 13, 14 [2] |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
| 12, 14, 15 | ABI 3130 | ID |
Kristen Homich, Erie County Central Police Services Forensic Laboratory, Buffalo NY |
Re-amplification and electrophoresis | 1 |
13 reported tri-allelic patterns as of 08/04/2016
* Use the following abbreviations for amplification kit names:
- CO: Applied Biosystems COfiler
- PR: Applied Biosystems Profiler
- PS: Applied Biosystems Profiler Plus
- ID: Applied Biosystems Identifiler
- PP: Promega PowerPlex 16
- CV: Promega CTTV
- CA: Promega CTTA
- MP: Promega MonoPlex
- Other: Please specify
Last Updated: 08/04/2016