D13S317 Variants
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Allele Designation |
Allele Size |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
5 |
195.14 |
ABI 377 |
PS |
Albar Chavana |
Multiple amplifications |
Convicted offender |
1 |
5 [2] |
193.47 |
ABI 310 |
PS |
Nora Rankin Tucson PDCL |
Multiple sample source |
Victim |
1 person |
5 [3] |
193.67 |
ABI 310 |
PS |
Angela Z. Withrow M U F S C |
Re-extracted x 1 |
Convicted Offender |
6 in 3680 |
5 [4] |
164.01 |
ABI 310 |
PP16 |
Cyndi Hall Idaho State Police |
Multiple sample source |
|
1 |
5 [5] |
193.3 |
ABI 310 |
PS |
Cody Buller Arizona DPS |
Re-amplification, re-injecttion. Sampling of Parent and Sibling, Amplify w/alternate kit |
First seen in son. Profiling of Mother and Brother produced same OL |
3 |
5 [6] |
205.0 |
ABI 310 |
ID |
Cody Buller Arizona DPS |
Re-amplification, re-injection .Sampling of Parent and Sibling, Amplify w/alternate kit |
First seen in son. Profiling of Mother and Brother produced same OL |
3 |
5 [7] |
162.58 |
ABI 3100 |
PP16 |
Laura Kuyper M U F S C |
Amplified twice with PP 16 and once with ID |
|
10 |
5 [8] |
162.52 |
ABI 3100 |
PP |
Laura Kuyper MUFSC |
Amplification x2 |
|
12 |
5 [9] | 204.73 | ABI 3130XL | ID | Jennifer Montgomery, MA State Police Forensic and Technology Center | Re-ran to confirm | Convicted Offender | 1 |
5 (<8) | 204.53 | ABI 3100 | ID |
Angela Withrow M U F S C |
|||
5 (<8) [2] | 193.53 | ABI 3100 | PS | Laura Kuyper M U F S C | |||
5 (<8) [3] | 193.52 | ABI 3100 | PS | Laura Kuyper M U F S C | |||
5 (<8) [4] | 193.6 | ABI 3100 | PS | Kelly Beatty M U F S C | |||
5 (<8) [5] | 193.52 | ABI 3100 | PS | Laura Kuyper M U F S C | |||
5 (<8) [6] | 193.67 | ABI 3100 | PS | Angela Withrow M U F S C | |||
5 (<8) [7] | 193.65 | ABI 3100 | PS | CBA M U F S C | |||
5 (<8) [8] | 193.13 | ABI 3100 | PS | Misty Marra M U F S C | |||
5 (<8) [9] | 204.53 & 204.51 | ABI 3130XL | ID | Regina Robinson, KY State Police Forensic Lab, Frankfort KY | Re-analysis | ||
5 (<8) [10] | 205.26 | ABI 310 | ID | Carmen Alaez Verson, Department of Immunology and Immunogenetics, InDRE SSA, Mexico City, Mexico | Re-amplification and re-injection; profiling of mother and son produced the same off-ladder allele | Paternity case, Mexican Mestizo ethnicity | |
5 (<7) | 162.53 | ABI 3100 | PP | Laura Kuyper M U F S C | |||
5 (<7) [2] | 162.63 | ABI 3100 | PP | Laura Kuyper M U F S C | |||
5 (<7) [3] | 162.52 | ABI 3100 | PP | Laura Kuyper M U F S C | |||
5 (<7) [4] | 204.84 | ABI 3130 | ID | Pamela Calogero, St Louis Metropolitan Police Department Crime Lab | Re-amplified | reference standard | 1 |
6 |
197.46 |
ABI 310 |
PS |
Kelly Beatty M U F S C |
Re-extraction x 2 |
Convicted Offender |
1 in 3360 |
6 [2] |
197.50 |
ABI 310 |
PS |
Michelle Hassler San Diego Sheriff Dept |
Amplified with two systems |
Training/validation sample |
1 |
6 [3] |
208.68 |
ABI 310 |
ID |
Michelle Hassler San Diego Sheriff Dept |
Amplified with two systems |
Training/validation sample |
1 |
6 [4] |
199.16 |
ABI 377 |
PS |
Catherine Allor |
Re-amplified and Reanalyzed |
Paternity samples only |
1 in 16650 |
6 [5] |
208.89 |
ABI 310 |
ID |
Megan McCardle St. Louis Metropolitan PD |
Extracted twice & multiple sample source |
Victim |
1 |
6 [6] |
207.84 |
ABI 310 |
PP 16 |
Frederick Schunter Haemogenetic Lab, Germany |
Re-extraction, re-amplification |
Caucasian sample,paternity case |
1 in 2230 |
6 [7] |
249.78 |
ABI 310 |
ID |
Frederick Schunter Haemogenetic Lab, Germany |
Re-extraction, re-amplification |
Caucasian sample,paternity case |
1 in 2230 |
6 [8] |
197.07 |
ABI 3100 |
PS |
Jan Nicklas,VT Forensic Laboratory |
Re-amp |
|
|
6 [9] | 167.99 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood reference | 22 in 11000 |
6 [10] | 166.54 | ABI 3100xl | PP | Justin Godgy M U F S C | 4 | ||
6 [11] | 210.15 | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-amplified and re-analyzed with ABI 3730 | 2 in 31,330 | |
6 (<7) [12] | 208.71 | ABI 3130 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3130 | 1 in 31,330 | |
6 (<7) [13] | 210.62 | ABI 3730 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-analyzed with ABI 3730 | 1 in 31,330 | |
6 (<8) |
167.68 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and re-amplify |
Convicted Offender |
1 in 32660 |
6 (<8) [2] |
208.85 +/-0.44 |
ABI 3130XL |
ID |
Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE |
Re-amplified and re-analyzed |
|
3 in 11500 |
6 (<8) [3] | 196.95 | ABI 3100 | PS | Nicole Hahn M U F S C | Previously seen - no verification | 2 | |
6 (<8) [4] | 197.61 | ABI 3100 | PS | Laura Kuyper M U F S C | |||
6 (<8) [5] | 209.14 | ABI 310 | ID | Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country | Re-amplified and re-analyzed | Convicted offender sample | |
7 |
200.82 |
ABI 310 |
PS |
|
|
|
|
7 [2] |
201.34 |
ABI 310 |
PS |
Chad Hainley |
Re-amplified |
Convicted offender known |
1 |
7 [3] |
202.53 |
ABI 377 |
PS |
Igor Kornienko, Tatyana Tarasova 124 Armed Forces Central Forensic Identification Laboratory, DoD, Russia |
Re-extracted and re-amplified |
Russian samples only |
1 in 614 to date |
7 [4] |
213.45 |
ABI 3100 |
ID |
Faruk Asicioglu Dept of Forensic Biology Institute of Forensic Medicine,Ministry of Justice, Ýstanbul,Turkey |
Re-extracted and re-amplified |
Paternity sample |
1 |
7 [5] |
201.45 |
ABI 3100 |
PS |
Faruk Asicioglu Dept of Forensic Biology Institute of Forensic Medicine,Ministry of Justice, Ýstanbul,Turkey
|
Re-extracted and re-amplified |
Paternity sample |
1 |
7 [6] |
201.2 |
ABI 3100 |
PS |
Jan Nicklas,VT Forensic Laboratory |
Re-amp |
|
10 in 3750 |
7.1 |
202.77 |
ABI 310 |
PR, MP |
Re-amplified and verified on a gel |
|
|
|
7.1 [2] |
204.27 |
ABI 377 |
PS |
Catherine Allor |
Re-amplified and Reanalyzed |
Paternity samples only |
1 in 11100 |
7.1 (<8) |
201.88 |
ABI 3100 |
PS |
Nicole Hahn M U F S C |
Re-amplified for confirmation |
|
1 |
<8 |
204.52 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amp x 1 |
Alabama Department of Forensic Sciences Convicted Offender Sample |
11 in 15543 |
<8 [2] |
193.13 |
ABI 3100 |
PS |
Misty Marra M U F S C |
Re-amplified |
|
9 in 16,579 |
<8 [3] | 208.43 | ABI 3100 | ID | Angela Withrow M U F S C | |||
8.1 |
206.53 |
ABI 310 |
PS |
|
|
|
|
8.1 [2] | 217.88 | ABI 3130xl | ID | Monica Price, Washington State Patrol Crime Laboratory | Re-extraction | Convicted Offender | |
8.1 [3] | 217.75 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | |||
8.1 [4] | 217.75 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | observed in multiple samples | ||
8.2 | 119.68 | ABI 3500 | PowerPlex Fusion 6C | Anna Wilson, Washington State Patrol Crime Laboratory | Two extractions and re-injection | ||
9.1 | 221.54 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-injected | ||
10.3 | 227.43 | ABI 3100 | ID | Marsha Garcia, Orchid Cellmark, Nashville TN | Re-extracted | ||
11.1 |
188.83 |
ABI 3100 |
PP |
Charity Noreuil, IL State Police DNA Indexing Lab |
Re-amplification |
Convicted Offender Sample |
1 |
11.3 |
220 |
ABI 310 ABI 3100 |
PS |
Suzanna Ulery, FDLE, Tampa |
Re-ran sample. |
Also have had multiple forensic unknowns from same case with matching profile. Suspect standard obtained (after CODIS hit) and also has the same confirmed OLA |
1 |
12.3 | 235.78 & 193.64 | ABI 310 | ID & PP | Lynette Stargardt, Independent Forensics. Hillside IL | Re-extracted | Paternity: not shared with tested child | |
12.3 [2] | 235.21 | ABI 3100 | ID | Lynette Stargardt, Independent Forensics. Hillside IL | Re-extracted | Paternity: not shared with tested child | |
12.3 [3] | 223.75 | ABI 3130 | PS | Laboratoire de sciences judiciaires et de médecine légale, Montréal, Canada | Re-amplified | 3 times in the same case | |
13.2 | 331.2 | ABI 3500xl | Promega Fusion 5C (see notes) | Shannon Keeton, Kentucky State Police Central Forensic Lab | Offender sample only processed once in Fusion 5C. A matching casework sample also processed in 5C matched the 13.2 but was the one mismatch with the original ID profile (see notes). | This sample was originally processed in ID with the allele calling 14 (240.3bp) on a 3130XL. During hit confirmation process using 5C, the discrepancy between the original ID and 5C profiles was noted. The sample was then processed using GlobalFiler Express, which typed the allele 14 (234.9bp) as seen in the older Life Tech chemistry. | 1 |
13.3 |
199.05 |
ABI 310 |
PP 16 |
|
|
|
|
13.3 [2] |
196.83 |
ABI 377 |
PP 1.2 |
Greg Sykes ATCC |
Re-extracted and re-amplified |
Cervical carcinoma, ATCC cell line CCL-2; name: HeLa. The 13.3 microvariant found in all HeLa derivatives and contaminants examined to date |
|
13.3 [3] |
239.32 |
ABI 3100 |
ID |
Angela Withrow M U F S C |
Re-amp x 1 |
AL Dept of Forensic Sciences Convicted Offender Sample |
1 in 16334 |
13.3 [4] | 239.64 | ABI 310 | ID | Oscar García, Forensic Genetics Department, Autonomous Police of the Basque Country | Reamplified and reanalyzed | Convicted offender sample | |
14.3 |
201.83 |
ABI 310 |
PP 1.2 |
Greg Sykes |
Re-extracted and re-amplified |
from cell line EPLC-32M1 (submitted as squamous cell carcinoma); this cell line has a similar (but not identical) PP profile to HeLa |
|
16 (>15) |
207.85 |
ABI 310 |
PP 16 |
Jackie Johnson MO State Highway Patrol |
Re-extract and Re-amplify |
Convicted Offender |
1 in 33739 |
16 |
237.19 |
ABI 310 |
PS |
Re-amplified and verified |
|
|
|
16 [2] |
236.97 |
ABI 3100 |
PR |
Faruk Asicioglu |
Re-extracted and re-amplified |
|
1 |
16 [3] |
237.01 |
ABI 3100 |
PS |
Jan Nicklas,VT Forensic Laboratory |
Re-amp |
|
|
16 [4] |
208.05
|
ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood reference | 16 in 11000 |
16 [5] | 248.77 | ABI 310 | ID | Oscar Garcia, Forensic Genetics Dept, Autonomous Police of the Basque Country, SPAIN | Re-amplified and re-analyzed | Criminal case sample | |
16 [6] | 248.5 | ABI 3130xl | ID Plus | S. Frackowiak, Forensic Lab INPS/LPS Marseille, FRANCE | observed in 3 multiple samples obviously coming from the same person | first detected in DNA from 2 saliva samples of a single individual; detected a few years later in DNA extracted from a semen stain, which totally matched in the national database with the previous individual | 1 person |
17 |
251.96 |
ABI 3100 |
ID |
R. Vincent Miller, Chromosomal Laboratories, Inc., Phoenix AZ |
Re-extracted and re-amplified |
|
1 |
17 [2] | 212.09 | ABI 3100 | PP | ICMP | Re-extraction and amplification or observed intergenerational inheritance | Blood reference | 1 in 11000 |
19 reported variants as of 11/15/2017
Tri-Allelic Patterns
Allele Designation |
Instrument |
Amp Kit* |
Contributor |
Verification/Conformation Method(s) |
Notes |
Frequency |
5, 8, 13 | ABI 3500 | Applied Biosystems GlobalFiler | Ellyn Colquhoun Monroe County Crime Laboratory | Re-amp x 1 | Allele Size: 198.67, 210.84, 231.09 - Criminal case sample, dental floss. A tri-allelic pattern also observed at vWA | 0 |
8, 9, 10 | ABI 3130xl | GlobalFiler Express | Su-Qing Gao - Shenzhen Blood Center, Paternity Lab, China | PowerPlex 21 | Allele Size: 8 [210.64-210.74], 9 [214.67-214.7], 10[218.72-218.83] | 1 in 3100 |
8, 9, 11 | ABI 3130 | ID | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-amplified and re-analyzed with ABI 3130 | 1 in 31,330 | |
8, 9, 11 [2] | ABI 3500 | GlobalFiler | Meagan Lesser; West Virginia State Police Forensics Laboratory; Biochemistry Section | re amplified | Convicted Offender - Allele Size: 210.8, 214.83, 223 | 1 |
8, 9, 12 | ABI 3130xl | ID | Sherri Phillips, Washington State Patrol CODIS Lab | Re-extracted | ||
8, 9, 13 | ABI 3130 | Sinofiler | Yunfei An & Jun Wang, Department of Laboratory Medicine, west china hospital, Sichuan University Chengdu, Sichuan, PR China | Re-extraction and reamplification | Type I tri-allelic pattern with peak height of 8 equaling peak heights for 9 +13 , paternity case: father 8,9 child:8,9,13 | 1 in 1874 cases |
8, 10, 11 | ABI 3500xl | ID | Robert Dorion, Washington State Patrol Crime Laboratory | Re-extraction | Convicted Offender | |
8, 11, 12 |
ABI 310 |
PS |
Jenifer Palmer Phoenix Police Department |
Re-extracted and re-amplified |
|
|
8, 11, 12 [2] | ABI 3100 | PP | Laura Kuyper M U F S C | Amplification x 2 | 1 | |
8, 11, 12 [3] | ABI 310 | ID | Oscar Garcia, Forensic Genetics Department, Autonomous Police of the Basque Country | Re-amplified and Re-analyzed | Convicted offender sample from the Forensic Genetics Department, Autonomous Police of Basque Country | |
8, 11, 12 [4] | ABI 3500XL | Promega PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Type 1, Convicted offender | |
8, 11, 13 | ABI 3130xl | ID | Franck Cima, Francis Hermitte, Sylvain Hubac and Frederic Brard, National Forensic Science Institute of the French Gendarmerie. IRCGN, Rosny-sous-Bois, France | male reference sample on FTA (buccal cells) extracted and amplified twice separately |
8 [216.39-216.69], 11 [228.33-228.61], 13[236.15-236.51] TH01 (6) and D16 (12) both homozygous |
|
8,13,14 | ABI 310 | PP16 & ID |
Dr.
Ulrike Schacker Director DNA Laboratory Galantos Genetics GmbH Campus Universität-Mainz Johann-Joachim-Becher-Weg 30a 55128 Mainz Germany |
Re-extraction and reamplification with Identifiler and PP16 |
Type I tri-allelic pattern with peak height of 8
equaling peak heights for 13 +14
paternity case: father 8,12 mother 11,13 child: 8,13,14 |
1 in 1500 paternity cases |
9, 10, 11 | ABI 3100 | PP | Sun Hongyu, Chen Wenjing, Xue Tianyu. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China | Re-extraction, re-amplification with PP16, confirmation with Sinofiler Kit (Applied Biosystems) and AGCU 17+1 STR Kit (AGCU ScienTech, China) | Paternity Samples, Type 2 | 1 in 28252 |
9, 11, 12 | ABI 3130xl | ID | Sherri Phillips, Washington State Patrol CODIS Crime Laboratory | Re-extracted | ||
9, 12, 13 | ABI 3500 | Identifiler | Juan R. Delgado. Fiscalia General de Justicia del Estado de Nuevo Leon, Mexico. | Re-extraction and re-amplification | Type I tri-allelic pattern with peak height of 9 equaling peak heights for 12+13. | 1 |
9, 13, 14 | ABI 3500 | GlobalFiler | Brenda Crosby, Iowa DCI Crime Laboratory | Re-amplified | Seen in known buccal sample - Allele Size: 9 (214.89), 13 (231.12), 14 (235.15) | |
10, 11, 12 |
ABI 377 |
PS |
Melinda Matte & Sarah Johnston Centre of Forensic Sciences, Toronto, Ontario, CANADA |
Re-amplification |
Occurred in two separate cases--were linked together via match on the Crime Scene Index of the National DNA Databank |
|
10, 11, 12 [2] |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
10, 11, 12 [3] | ABI 3500 | ID, PP16 HS | DNA Forensic Division, Supreme Prosecutor's Office, South Korea | Re-amplified and re-analyzed with ABI 3500 | 1 in 31,330 | |
10, 11, 12 [4] | ABI 310 | ID | Vicki Yarnell, Indiana Blood Center, Paternity Lab | Re-amplified and re-analyzed | Seen in child of paternity trio-Mother 10,12/ Father 9,11 | |
10, 11, 12 [5] | ABI 3500 | Globalfiler | Ian Perry; West Virginia State Police Forensic Lab, Biology Unit: Databasing Section | re-extraction and reamplification | Offender Sample - Allele Size: 219.01, 223.02, 227.02 | 1 |
10, 11, 15 |
ABI 310 |
PS |
Alison Reener NE State Patrol Crime Lab |
Re-extracted and Re-amplified |
Reference sample not matching evidence |
1 |
10, 12, 13 |
ABI 377 ABI 310 |
PS |
William Stubbs Washington SP |
Re-extracted and amplified |
Convicted offender |
1 in 30,000 |
10, 12, 13 [2] | ABI 3500xl | ID | Monica Price, WA State Patrol Crime Lab | Re-extraction | Convicted offender | |
10, 12, 17 |
ABI 3100 |
ID |
R. Vincent Miller,Chromosomal Laboratories, Inc., Phoenix AZ |
Re-extracted and re-amplified |
|
1 |
11, 12, 13 |
ABI 3100 |
ID & PS |
Angela Withrow M U F S C |
Re-amp x 1 with ID and once with PS |
AL Depart of Forensic Sciences Convicted Offender sample |
1 in 16046 |
11, 12, 13 [2] |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
11, 12, 13 [3] | ABI 3500XL | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-injection | Convicted offender, Type 1 | |
11, 12, 14 |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
11, 12, 14 [2] | ABI 310 | ID | King_C USACIL | Re-extracted and re-amplified | Reference Blood | |
11, 13, 14 | ABI 3500xl | PowerPlex Fusion 6C | Monica Price, WA State Patrol Crime Lab | Re-direct amp | Type 1, Convicted offender | |
11, 14, 15 | ABI 310 | Brenda Markham, Fresno County Sheriff's Department | Blood (tested in duplicate) Saliva (tested in duplicate) | Type I Tri Allelic (peak heights of "14" + "15" are roughly equal to the height of the "11" | l | |
13, 14, 15 |
ABI 310 |
PP 16 |
Malena B. Jimenez, MO State Highway Patrol |
Re-extract & Re-amplify |
Convicted Offender |
1 in 69600 |
16 reported tri-allelic patterns as of 01/31/2017
* Use the following abbreviations for amplification kit names:
- CO: Applied Biosystems COfiler
- PR: Applied Biosystems Profiler
- PS: Applied Biosystems Profiler Plus
- ID: Applied Biosystems Identifiler
- PP: Promega PowerPlex 16
- CV: Promega CTTV
- CA: Promega CTTA
- MP: Promega MonoPlex
- Other: Please specify