Study Completed

Description of Samples Tested (performed in 7 labs and Promega)

# Run

Single Source (Concordance)

5 samples x 8 labs

40

Mixture Ratio (male:female)

6 labs x 2 M/F mixture series x 11 ratios (1:0,1:1,1:10,1:100,1:300,1:1000,0.5:300, 0.25:300,0.125:300, 0.0625:300, 0.03:300 ng M:F)

132

Mixture Ratio (male:male)

6 labs x 2 M/M mixtures series x 11 ratios (1:0,19:1,9:1,5:1,2:1,1:1,1:2,1:5,1:9,1:19,0:1)

132

Sensitivity

7 labs x 2 series x 6 amounts (1/0.5/0.25/0.125/0.06/0.03)

84

Non-Human

24 animals

24

NIST SRM

6 components of SRM 2395

6

Precision (ABI 3100 and ABI 377)

10 ladder replicates + 10 sample replicated + [8 ladders + 8 samples for 377]

36

Non-Probative Cases

65 cases with 102 samples

102

Stutter

412 males used

412

Peak Height Ratio

N/A (except for DYS385 but no studies were noted)

Cycling Parameters

5 cycles (28/27/26/25/24) x 8 punch sizes x 2 samples

80

Annealing Temperature

5 labs x 5 temperatures (54/58/60/62/64) x 1 sample

25

Reaction volume

5 volumes (50/25/15/12.5/6.25) x [5 amounts + 5 concentrations]

50

Thermal cycler test

4 models (480/2400/9600/9700) x 1 sample + [3 models x 3 sets x 12 samples]

76

Male-specificity

2 females x 1 titration series (0-500 ng female DNA) x 5 amounts

10

TaqGold polymerase titration

5 amounts (1.38/2.06/2.75/3.44/4.13 U) x 4 quantities (1/0.5/0.25/0.13 ng DNA)

20

Primer pair titration

5 amounts (0.5x/0.75x/1x/1.5x/2x) x 4 quantities (1/0.5/0.25/0.13 ng DNA)

20

Magnesium titration

5 amounts (1/1.25/1.5/1.75/2 mM Mg) x 4 quantities (1/0.5/0.25/0.13 ng DNA)

20

TOTAL SAMPLES EXAMINED

1269