PowerPlex Y Validation
Reference: Krenke et al. (2005) Forensic Sci. Int. 148(1):1-14
Study Completed |
Description of Samples Tested (performed in 7 labs and Promega) |
# Run |
Single Source (Concordance) |
5 samples x 8 labs |
40 |
Mixture Ratio (male:female) |
6 labs x 2 M/F mixture series x 11 ratios (1:0,1:1,1:10,1:100,1:300,1:1000,0.5:300, 0.25:300,0.125:300, 0.0625:300, 0.03:300 ng M:F) |
132 |
Mixture Ratio (male:male) |
6 labs x 2 M/M mixtures series x 11 ratios (1:0,19:1,9:1,5:1,2:1,1:1,1:2,1:5,1:9,1:19,0:1) |
132 |
Sensitivity |
7 labs x 2 series x 6 amounts (1/0.5/0.25/0.125/0.06/0.03) |
84 |
Non-Human |
24 animals |
24 |
NIST SRM |
6 components of SRM 2395 |
6 |
Precision (ABI 3100 and ABI 377) |
10 ladder replicates + 10 sample replicated + [8 ladders + 8 samples for 377] |
36 |
Non-Probative Cases |
65 cases with 102 samples |
102 |
Stutter |
412 males used |
412 |
Peak Height Ratio |
N/A (except for DYS385 but no studies were noted) |
|
Cycling Parameters |
5 cycles (28/27/26/25/24) x 8 punch sizes x 2 samples |
80 |
Annealing Temperature |
5 labs x 5 temperatures (54/58/60/62/64) x 1 sample |
25 |
Reaction volume |
5 volumes (50/25/15/12.5/6.25) x [5 amounts + 5 concentrations] |
50 |
Thermal cycler test |
4 models (480/2400/9600/9700) x 1 sample + [3 models x 3 sets x 12 samples] |
76 |
Male-specificity |
2 females x 1 titration series (0-500 ng female DNA) x 5 amounts |
10 |
TaqGold polymerase titration |
5 amounts (1.38/2.06/2.75/3.44/4.13 U) x 4 quantities (1/0.5/0.25/0.13 ng DNA) |
20 |
Primer pair titration |
5 amounts (0.5x/0.75x/1x/1.5x/2x) x 4 quantities (1/0.5/0.25/0.13 ng DNA) |
20 |
Magnesium titration |
5 amounts (1/1.25/1.5/1.75/2 mM Mg) x 4 quantities (1/0.5/0.25/0.13 ng DNA) |
20 |
|
TOTAL SAMPLES EXAMINED |
1269 |
Comments: