| Study Conducted | Description of Samples Run with PowerPlex 16 Validation | # Run | If redone now |
| Single Source (Concordance) | 8 samples (Promega concordance) + 200 samples (part of population concordance study) | 208 | 100 |
| Mixtures | 45 | 45 | 10 |
| Mixture Ratio | 1 sample x 11 ratios (1:0, 19:1, 9:1, 4:1, 2:1, 1:1, 1:2, 1:4, 1:9, 1:19, 0:1) x 2 injections (5/10 seconds) | 22 | 33 |
| Sensitivity | 5 samples x 8 amounts (5/2/1/0.5/0.25/0.125/0.06/0.03 ng) + [5 samples x 3 points (at/above/below dropout)] | 55 | 33 |
| Non-Human | 11 animals | 11 | 0 |
| NIST SRM 2391b | 12 components | 12 | 12 |
| Precision (ABI 310) | (5 samples x 10 injections each) + 10 injections of allelic ladders | 60 | 60 |
| Non-Probative Cases | 5 cases x 4 samples each (evidence EF&SF/victim/suspect) | 20 | 20 |
| Stutter | 200 samples (data used from population samples) | - | - |
| Peak Height Ratio | 200 samples (data used from population samples) | - | - |
| Cycling Parameters | 14 samples x 2 different cycle numbers (30/32) x 2 injection times (3/5 seconds) | 56 | - |
| Annealing Temperature | 3 samples x 4 concentrations (2.0/1.0/0.5/0.25 ng) x 5 temperatures (56/58/60/62/64) | 60 | 0 |
| Proficiency | 9 sets x 4 samples per set | 36 | 12 |
| Substrate | 9 common substrates x 1 sample each | 9 | 0 |
| Environment | 5 conditions (outside/80oC/50oC/4oC/RT) x 6 time points (3/6/12/25/48/85 days) | 30 | 0 |
| Various tissues | Bone, hair, teeth, semen, perspiration, urine, blood, semen, vaginal swab (minimum of one sample each) | 9 | 0 |
| TOTAL SAMPLES RUN | 633 | 280 |