Margaret C. Kline
Research Biologist

Human Identity Project Team
DNA Measurements Group
Biochemical Science Division

Chemical Science & Technology Laboratory

National Institute of Standards and Technology
100 Bureau Drive, Mail Stop 8311
Gaithersburg, M
D 20899-8311, USA



Phone: 301-975-3134        

Fax: 301-975-8505




B.S.     Magna Cum Laude, Animal Science/Veterinary Science, University of Maryland (College Park), 1976                   

M.S.    Animal/Dairy/Veterinary Science, Food Science, University of Maryland (College Park), 1979





2000  American Statistical Association W.J. Youden Award in Interlaboratory Testing. 

For the paper entitled “Micronutrients Measurement Quality Assurance Program: Helping Participants Use Interlaboratory Comparison Exercise Results to Improve Their Long-Term Measurement Performance” Analytical Chemistry, Vol. 71, No. 9, May 1, 1999, pp. 1870-1878.  Authors: David L. Duewer, Margaret C. Kline, Katherine E. Sharpless, Jeanice Brown Thomas, Kenneth T. Gary, and Anne Sowell.

1995  Department of Commerce Bronze Medal Award. 

For the development of SRM 2391, PCR-based DNA Profiling Standard which provides quality assurance for forensics and other human identification efforts.




American Chemical Society




Many of these articles are available as pdf files at

Hill, C.R., Kline, M.C., Mulero, J.J., Lagace, R.E., Chang, C.-W., Hennessy, L.K., Butler, J.M. (2007) Concordance study between the AmpFlSTR MiniFiler PCR Amplification Kit and conventional STR typing kits. J. Forensic Sci. 52(4): 870-873.


Barni, F., Salata, E., Rapone, C., Kline, M., Lago, G., Butler, J., Berti, A. (2007) Characterisation of novel alleles and duplication events in the STR DYS19, DYS439, DYS389II and DYS385 loci. Identity (Promega Newsletter for Europe, Middle East and Africa), Spring 2007, pp. 1-3.


Decker, A.E., Kline, M.C., Vallone, P.M., Butler, J.M. (2007) The impact of additional Y-STR loci on resolving common haplotypes and closely related individuals. FSI Genetics 1:215-217.


Decker, A.E., Kline, M.C., Redman, J.W., Reid, T.M., Butler, J.M. (2007) Analysis of mutations in father-son pairs with 17 Y-STR loci. submitted.


Coble, M.D., Kline, M.C., Redman, J.W., Decker, A.E., Vallone, P.M., Butler, J.M. (2006) An investigation of discrimination capacity and the cause of null alleles in Linear Array mitostrips using control region sequence data. submitted.


Butler, J.M., Coble, M.D., Decker, A.E., Duewer, D.L., Hill, C.R., Kline, M.C., Redman, J.W., Vallone, P.M. (2006) Setting standards and developing technology to aid the human identity testing community. Progress in Forensic Genetics 11, Elsevier Science: Amsterdam, The Netherlands, International Congress Series 1288, 628-635.


Butler, J.M., Decker, A.E., Vallone, P.M., Kline, M.C. (2006) Allele frequencies for 27 Y-STR loci with U.S. Caucasian, African American, and Hispanic samples, Forensic Sci. Int.156:250-260.


Kline, M.C., Vallone, P.M., Decker, A.E., Redman, J.W., Duewer, D.L., Butler, J.M. (2005) Testing candidate DNA quantitation standards with several real-time quantitative PCR methods. Proceedings of the 16th International Symposium on Human Identification.


John M. Butler, Amy E. Decker, Kline, Margaret C., and  Peter  M. Vallone (2005) Chromosomal Duplications Along the Y-Chromosome and Their Potential Impact on Y-STR Interpretation, J. Forensic Sci: 50(5): 1061-1070


Kline, Margaret C. M.S.; David L. Duewer, Ph.D.; Janette W. Redman; and John M. Butler, Ph.D

Results from the NIST 2005 DNA Quantitation Study J. Forensic Sci J. Forensic Sci., 50(3): 571-578.


Kline, Margaret C. , Peter  M. Vallone, Janette W. Redman; David L. Duewer,  Cassandra D. Calloway, and John M. Butler (2005) Mitochondrial DNA Typing Screens with Control Region and Coding Region SNPs, J. Forensic Sci. 50: 377-385.


Duewer , D.L., Kline M.C., Redmam, J.W., and Butler, J.M. (2004) NIST Mixed Stain Study#3: Signal Intensity Balance in Commercial Short Tandem Repeat Multiplexes Anal. Chem 76, 6928-6934.


Schoske, R., Vallone, P.M., Kline, M.C., Redman, J.W., Butler, J.M. (2004) High-throughput Y-STR typing of U.S. populations with 27 regions of the Y chromosome using two multiplex PCR assays, Forensic Sci. Int. 139: 107-121.


Kline, M.C., Duewer, D.L., Butler, J.M. (2003) Biotech SRMs designed for the rapidly evolving forensic DNA and human identity testing communities. NCSL International 2003 Workshop and Symposium Conference Proceeding: The Spectrum of Metrology: From the State-of-the-Art to the Everyday. See


Butler John M., Richard Schoske, Peter M. Vallone, Janette W. Redman, and Margaret C. Kline, Allele Frequencies for 15 Autosomal STR Loci on U.S. Caucasian, African American, and Hispanic Populations J. Forensic Sci. Vol. 48 (4) pp908-911.


Kline Margaret C, David L Duewer, JanetteW Redman, John M Butler.  NIST Mixed Stain Study #3: DNA Quantification Practice and it’s Influence on Short Tandem Repeat Multiplex Performance. Anal Chem, 2003 , Vol 75 (10), pp 2463-2469.


Butler John M., Richard Schoske, Peter M. Vallone, Margaret C. Kline, Alan J. Redd, and Michael F. Hammer A Novel Multiplex for Simultaneous Amplification of 20 Y Chromosome STR Markers Forensic Science International 2002 Vol. 129 (1) pp10-24.


Kline Margaret C., David L. Duewer, Janette W. Redman, John M. Butler, and David A. Boyer. Polymerse Chain Reaction Amplification of DNA from Aged Blood Stains: Quantitative Evaluation of the “Suitability for Purpose” of Four Filter Papers as Archival Media Analytical Chemistry,2002, 74, 1863-1869.


Bulbin S. Akbasak, Bruce Budowle, Dennis J Reeder, Janette W Redman, and Margaret C Kline. Turkish Population Data with the CODIS Multiplex Short Tandem Repeat Loci Forensic Science International 123 (2001) 227-229.


Barbara C. Levin, Haiyan Cheng, Margaret C Kline, Janette W Redman and Kristy L Richie. A review of DNA Standard Reference Materials Development by the National Institute of Standards and Technology Fresenius' J. Anal Chem 2001, 370: 213-219.


David L. Duewer, Margaret C Kline, Janette W. Redman, Pamela J. Newall. 2000 NIST Mixed Stain Studies #1 and #2:  Interlaboratory Comparison of DNA Quantification Practice and Short Tandem Repeat Multiplex Performance with Multiple-Source Samples J Forensic Sci 2001 ;46(5):191-202.


Jeanice Brown-Thomas, Margaret C. Kline, Lisa M. Gill, James H. Yen, David L. Duewer, Lorna T. Sniegoski, and Katherine E. Sharpless. Preparation and Value Assignment of Standard Reference Material 968c Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum Clinica Chimica Acta.2001 Vol 305 pp 141-155


David L. Duewer,  Margaret C. Kline,  Katherine E. Sharpless, and Jeanice Brown Thomas.NIST Micronutrients Measurement Quality Assurance Program: Characterizing the Measurement Community’s Performance over Time Anal. Chem. 2000, 72(17); 4163-4170.


David L. Duewer,  Margaret C. Kline,  Katherine E. Sharpless, Jeanice Brown Thomas, Maria Stacwicz-Sapuntzakis; Anne L. Sowell. NIST Micronutrients Measurement Quality Assurance Program: Characterizing Individual Participant Measurement Performance over Time Anal. Chem 2000 Vol 72 No. 15, pp 3611-3619.


David L. Duewer,  Margaret C. Kline,  Katherine E. Sharpless, Jeanice Brown Thomas, Kenneth T. Gary; Anne L. Sowell. 1999. Micronutrients Measurement Quality Assurance Program: Helping Participants Use Interlaboratory Comparison Exercise Results to Improve Their Long-term Measurement Performance. Anal. Chem. (1999), 71(9), 1870-1878.


Jeanice Brown Thomas, David L. Duewer, Margaret C. Kline, and Katherine E. Sharpless. 1998. The Stability of retinol, a-tocopherol, trans-lycopene, and trans-b-carotene in liquid-frozen and lyophilized Serum. Clinica Chimica Acta.1998; Vol.276(1) pp. 75-87.


David L. Duewer, Jeanice Brown Thomas, Margaret C. Kline, William A. MacCrehan, Robert Schaffer, Katherine E. Sharpless, and Willie E. May. 1997. NIST/NCI Micronutrients Measurement Quality Assurance Program: Measurement Reproducibility, Repeatability, Stability, and Relative Accuracy for Fat-Soluble Vitamin-Related Compounds in Human Sera.  Analytical Chemistry; Vol. 69, no. 7, pp 1406-1413.


Margaret C. Kline, David L. Duewer, Pamela Newall, Janette W. Redman, Dennis J. Reeder, and Melanie Richard. 1997. Interlaboratory Evaluation of Short Tandem Repeat Triplex CTT. J. Forensic Sciences. Vol. 42, no. 5, pp 897-906.


Thomas J.B., M.C. Kline, S.B. Schiller, P.M. Ellerbe, L.T. Sniegoski, D.L. Duewer, and K.E.  Sharpless. 1996.  Certification of Fat - Soluble Vitamins and Cholesterol in Human Serum:    Standard Reference Material 968b. Fres. J. Anal. 356(1): 1-9


Margaret C. Kline, Janette W. Redman, Dennis J. Reeder & David L. Duewer. 1996. Intercomparison of DNA sizing ladders in electrophoretic separation matrices and their potential for accurate typing of the D1S80 locus. Applied and Theoretical Electrophoresis 6(1), 33-41.


Reeder D. J., M.C. Kline, and K.L. Richie. 1995.  An overview of reference materials prepared for standardization of DNA typing procedures. Fres. J. Anal. Chem. 352:246-249.


Srinivasan K., J.E. Girard, P. Williams, R.K. Roby, V. W. Weedn, S.C. Morris, M.C. Kline and D.J. Reeder. 1993. Electrophoretic separations of polymerase chain reaction (PCR)-amplified DNA fragment in DNA typing using a capillary electrophoresis-laser induced fluorescence system (CE-LIF).  J. Chromatogr. A. 652:83-91.


Srinivasan K., S.C. Morris, J.E. Girard, M.C. Kline, and D.J. Reeder. 1993.  Enhanced detection of PCR products through use of TOTO and YOYO intercalating dyes with laser induced fluorescence - capillary electrophoresis.  Applied and Theoretical Electrophoresis 3:235-239.


Reeder D.J., and M.C. Kline. 1991. Questionable use of DNA standards for sizing PCR products in small gels.  Crime Laboratory Digest 18:201.


R.C. Pandey, C. Kalita, M.E. Gustafson, M.C. Kline, M.E. Leidhecker, and J.T. Ross. 1985  "Process Developments in the Isolation of Largomycin F-II, a Chromoprotein Anti-tumor Antibiotic. " In: ACS Symposium Series; No. 271  Purification of Fermentation Products: Applications to Large Scale Processes.


M.C. Kline, A.W. Kotula and E.P.Young; 1985. Effect of Aqueous Chlorine (600 ppm) on the Protein  and the Lipid Fractions of Ground Beef.  J. of Food Quality. 7 (1985) 229-235.


Letter to the Editor: Non-Amplification of a vWA Allele.  Margaret C. Kline, and Brad Jenkins.  J. Forensic Science 1998, Vol 43, No. 1 p250.





For list of recent presentations (numerous  invited talks given since 2000), see