Mitochondrial DNA LINEAR ARRAY Evaluation

Participants:   Margaret C. Kline, Janette W. Redman, Peter M. Vallone, David L. Duewer, and John M. Butler

Project Timeframe: July 2002-Oct 2003

Purpose: Beta-testing of HVI/HVII mtDNA LINEAR ARRAYs (Roche Applied Sciences, Indianapolis, IN), and automation of the process. The LINEAR ARRAYS offer forensic laboratories doing human identification a rapid test for screening samples that may or may not require labor-intensive full sequencing.

Progress: Automation of LINEAR ARRAY processing was evaluated using a Tecan/Profiblot hybridization robot. Mitochondrial DNA mitotypes/haplotypes were established for 128 Hispanic, 252 African American, and 286 Caucasian population samples by automating the LINEAR ARRAY processing. These samples resulted in 282 different types of which 185 were unique (seen once in the NIST U.S. population samples). These unique samples represent 65.6% of the types seen and 27.8% of the people. Several groupings of samples seem to represent specific ethnic groups. The most common type, which consisted of 51 samples, was mitotype 1111111111 by LINEAR ARRAY analysis.  After an additional SNP typing assay, the 51 samples could be sub-divided into 12 different groups with four samples being unique.

Publications or Presentations Resulting from this Project:

Margaret Kline poster at 14th International Symposium on Human Identification (Phoenix, AZ), September 30-October 2, 2003, "Semi-Automation of mtDNA Arrays: Results from 666 Population Samples and Comparisons" [.pdf]

Kline, M.C., Vallone, P.M., Redman, J.W., Duewer, D.L., Calloway, C.D., and Butler, J.M. (2005) Mitochondrial DNA typing screens with control region and coding region SNPs. J. Forensic Sci. 50: 377-385.

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Last updated: 06/19/2007

Disclaimer: This project was supported by National Institute of Justice Grant Number 2003-IJ-R-029, which is an interagency agreement between NIJ and the NIST Office of Law Enforcement Standards, awarded by the National Institute of Justice, Office of Justice Programs, US Department of Justice. Points of view in this document are those of the authors and do not necessarily represent the official position or policies of the US Department of Justice. Certain commercial equipment, instruments and materials are identified in order to specify experimental procedures as completely as possible.  In no case does such identification imply a recommendation or endorsement by the National Institute of Standards and Technology nor does it imply that any of the materials, instruments or equipment identified are necessarily the best available for the purpose.